سنوروزیس تجربی مغزی و غیر مغزی ناشی از تنیا مولتی‌سپس در حیوانات آزمایشگاهی

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: سنوروزیس، به عنوان مرحله نوزادی تنیا مولتی‌سپس، یکی از بیماری‌های انگلی مهم در دامپزشکی است. هدف: این مطالعه با هدف بررسی این موضوع انجام شد که آیا خرگوش‌ها، موش‌های صحرایی و موش‌های آزمایشگاهی نسبت به آلودگی تجربی با تنیا مولتی‌سپس حساس هستند و می‌توانند مهاجرت و/یا تکامل مرحله لاروی این انگل را پس از خوراندن تخم‌های به‌دست آمده از کرم‌های بالغ منشأ گرفته از سنوروس‌های مغزی و عضلانی پشتیبانی کنند یا خیر. روش کار: تخم‌های جدا شده از کرم‌های بالغ حاصل از کیست‌های مغزی و عضلانی به صورت خوراکی به دو گروه خرگوش، موش صحرایی و موش آزمایشگاهی خورانده شد. بررسی‌های هیستوپاتولوژیک بر روی بافت‌های مغز و کبد حیوانات آلوده شده به صورت تجربی انجام شد. همچنین، به منظور ردیابی انگل در بافت‌های آلوده بر اساس نشانگرهای DNA میتوکندریایی، واکنش زنجیره‌ای پلیمراز (PCR) با استفاده از پرایمرهای ژن‌های CO1 و ND1 انجام و به دنبال آن تعیین توالی صورت گرفت. نتایج: نتایج در کالبدگشایی و بررسی هیستوپاتولوژی نشان داد که در هیچ‌یک از بافت‌ها کیستی تشکیل نشده است. با این حال، در کبد هشت خرگوش آلوده، مسیر مهاجرت انگل همراه با ضایعات هیستوپاتولوژیک شامل تورم سلولی و خونریزی‌های کانونی مشاهده شد. آنالیز مولکولی نمونه‌های کبد خرگوش‌ها نیز حضور DNA انگل تنیا مولتی‌سپس (نشانگرهای میتوکندریایی CO1 و ND1) را در بافت‌های آلوده تایید نمود. در بافت‌های موش‌های صحرایی و موش‌های آزمایشگاهی، هیچ‌ تغییر آسیب‌شناسی یا بقایای انگلی مشاهده نشد. نتیجه‌گیری: خرگوش (Oryctolagus cuniculus) ممکن است بتواند به عنوان یک مدل حیوانی بالقوه برای مطالعه جنبه‌های خاصی از پاتوژنز سنوروزیس مورد استفاده قرار گیرد. مطالعات بیشتری برای روشن شدن نقش فاکتورهای اختصاصی میزبان، وضعیت سیستم ایمنی، سویه‌های انگل و روش‌های ایجاد سنوروزیس تجربی ضروری است.

کلیدواژه‌ها

موضوعات


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