آنتی اکسیدان Mito-TEMPO سبب بهبود نگهداری اسپرم سرد شده سگ در یک دوره ذخیره سازی 72 ساعته می‌شود

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: تأثیر مثبت Mito-TEMPO، به عنوان یک آنتی اکسیدان هدفمند میتوکندریایی، بر نگهداری اسپرم در گونه‌های مختلف گزارش شده است، اما در این ارتباط اطلاعات در دسترس در گونه سگ محدود است. هدف: مطالعه حاضر به منظور ارزیابی تأثیر Mito-TEMPO بر نگهداری اسپرم سرد شده سگ در طول یک دوره 72 ساعته به انجام رسید. روش‌کار: در مطالعه اول تأثیر دوزهای صفر (به عنوان گروه شاهد)، 25، 50 و 100 میکرومولار از Mito-TEMPO بر تحرک، مورفولوژی، زنده‌مانی و یکپارچگی غشا پلاسمایی اسپرم در مقاطع زمانی ساعات صفر، 24، 48 و 72 بررسی شد. در مطالعه دوم تأثیر دوزهای صفر (به عنوان گروه شاهد) و 100 میکرومولار (به عنوان دوز موثر شناسایی شده در مطالعه اول) از Mito-TEMPO بر رادیکال‌های آزاد اکسیژن (ROS) و پتانسیل غشای میتوکندری در مقاطع زمانی ساعات صفر و 72 بررسی شد. نتایج: در مطالعه اول شاخص‌های تحرک، سرعت حرکتی، زنده‌مانی و نسبت اسپرم‌های با مورفولوژی طبیعی و غشای پلاسمایی سالم در طول زمان در تمامی گروه‌ها کاهش پیدا کرد (P<0.05)، اما این کاهش در اسپرم‌های تیمار شده با دوزهای مختلف Mito-TEMPO کندتر از اسپرم‌های تیمار نشده بود (P<0.05). در این ارتباط، کاهش حرکت تام و پیشرونده در گروه تیمار نشده از ساعت 24 به بعد مشاهده شد، اما این کاهش در گروه‌های تیمار شده با دوزهای مختلف Mito-TEMPO از ساعت 48 به بعد دیده شد (P<0.05). علی‌رغم اینکه تمامی دوزهای Mito-TEMPO تأثیر مثبت داشتند، تیمار با دوز 100 میکرومولار Mito-TEMPO بهترین کیفیت نگهداری را در مقایسه با گروه شاهد تأمین کرد (P<0.05). علاوه بر این در طول زمان نگهداری، در هر دو گروه شاهد و تیمار تولید ROS افزایش و پتانسیل غشای میتوکندری کاهش یافت (P<0.05)، اما تیمار با دوز 100 میکرومولار Mito-TEMPO این افزایش ROS و کاهش پتانسیل غشای میتوکندری در طول زمان را تخفیف داد (P<0.05). نتیجه‌گیری: Mito-TEMPO سبب بهبود شاخصه‌های ساختاری و عملکردی اسپرم سرد شده سگ در یک دوره 72 ساعته شد و می‌تواند یک آنتی اکسیدان موثر برای رقیق کننده اسپرم سگ باشد.

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موضوعات


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