تشخیص مولکولی و تعیین ژنوتیپ عفونت Theileria equi در جمعیت اسب‌ها در جیزه، مصر، با استفاده از real-time PCR در مقایسه با روش‌های تشخیص مرسوم

نوع مقاله : مقاله کامل

نویسندگان

10.22099/ijvr.2025.51028.7553

چکیده

پیشینه: پیروپلاسموز اسب یکی از مشکلات بهداشتی اصلی و جدی است که صنعت اسب را در سطح جهان تحت تاثیر قرار می‌دهد که توسط تک یاخته‌های Theileria equi و Babesia caballi ایجاد می‌شود. هدف: این مطالعه با هدف شناسایی و مشخص کردن ژنوتیپ T. equi در جمعیت اسب استان جیزه مصر با مقایسه نتایج به دست آمده با استفاده از روش‌های تشخیصی موجود انجام شد. روش کار: ما 116 اسب ظاهرا سالم را از منطقه مورد مطالعه در نیمه اول سال 2019 برای شناسایی T. equi با استفاده از PCR (qPCR)، با هدف قرار دادن ژن           18S rRNA جمع‌آوری کردیم. نتایج با نتایج حاصل از بررسی میکروسکوپی اسمیر خونی رنگ آمیزی شده با گیمسا و PCR معمولی مقایسه شد. ژنوتیپ توالی‌های به دست آمده نیز برای کشف تنوع ژنتیکی سویه‌های T. equi بررسی شدند. نتایج: نمونه‌های ما از طریق تعیین توالی و تجزیه فیلوژنتیک در خوشه‌های مربوط به ژنوتیپ A و ژنوتیپ E دسته‌بندی شدند. نتایج ما نشان داد که qPCR دارای بالاترین حساسیت (100%) و سپس PCR معمولی (68%) بود در حالی که بررسی میکروسکوپی کمترین حساسیت (38%) را داشت. علاوه بر این، ارزش اخباری منفی (NPV) qPCR بالاترین (100%) در مقایسه با PCR معمولی و معاینه میکروسکوپی (به ترتیب 49/80% و 04/68%) بود که نشان داد موارد منفی شناسایی شده توسط qPCR مطمئنا در مقایسه با دو روش تشخیصی دیگر درست است. نتیجه‌گیری: استفاده از روش‌های تشخیصی PCR در کنار بررسی میکروسکوپی برای ارزیابی وضعیت اپیدمیولوژیک پیروپلاسموز اسب بسیار توصیه می‌شود. همچنین، مطالعه ما نشان داد که ژنوتیپ A و ژنوتیپ E در میان اسب‌های مصری در گردش هستند.

کلیدواژه‌ها

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