تاثیر استفاده موضعی انسولین NPH بر بهبود زخم و استرس اکسیداتیو در سوختگی قلیایی قرنیه: یک مطالعه تجربی در مدل خرگوش

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: در سوختگی حاد قلیایی قرنیه، پروتکل درمانی معمول شامل سرکوب التهاب با استفاده از کورتیکواستروئیدها است. در سال‌های اخیر، علاقه فزاینده‌ای به نقش انسولین در تحقیقات ترمیم زخم به دلیل اثرات مثبت قابل توجه آن وجود داشته است. هدف: مطالعه حاضر با هدف مقایسه اثرات درمانی دگزامتازون به عنوان رویکرد مرسوم و انسولین NPH بر سوختگی قلیایی قرنیه انجام شد. روش کار: سوختگی قلیایی قرنیه با استفاده از Ca (OH)2 در همه حیوانات ایجاد شد. یک گروه (6 عضو) با پماد چشمی حاوی دگزامتازون (گروه I) و گروه دیگر (6 عضو) با پماد انسولین (گروه II) تحت درمان قرار گرفتند. گروه کنترل (6 عضو) هیچ درمانی دریافت نکردند (گروه III). تغییرات بالینی در نواحی سوختگی قرنیه در روزهای 3، 7، 14 و 21 بررسی شد. حیوانات در روز 21 با مرگ با شفقت از بین برده شدند. قرنیه‌های برداشته شده، از نظر تغییرات هیستوپاتولوژیک، ایمونوهیستوشیمیایی و بیوشیمیایی مورد بررسی قرار گرفتند. نتایج: نمودار بهبود بالینی موفق‌تری برای گروه دوم ترسیم شد. بهبود قابل توجهی (P<0.05) به خصوص در گروه II در روز 21 مشاهده شد. بالاترین میزان مثبت در رنگ آمیزی قرنیه MMP-9 در گروه اول مشاهده شد. گروه دوم افزایش قابل توجهی (P<0.05) در ظرفیت آنتی اکسیدانی کل (TAC) داشت. گروه‌های تیمار کاهش معنی‌داری (P<0.05) در مقادیر کل اکسیدان (TOC) و افزایش معنی‌داری (P<0.05) در سطوح کاهش یافته گلوتاتیون (GSH) را نسبت به گروه کنترل نشان دادند. نتیجه‌گیری: انسولین NPH موضعی منجر به بهبود بالینی سریع و بدون عارضه سوختگی قلیایی قرنیه شد. انسولین و دگزامتازون اثرات مشابهی داشتند نظیر افزایش مولکول‌های آنتی اکسیدان و کاهش مواد اکسیدان که نشان دادند انسولین ممکن است از تشکیل رادیکال‌های آزاد در قرنیه جلوگیری کند.

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موضوعات


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