اثرات سیتوتوکسیک و آپوپتوتیک زولدرونیک اسید بر رده سلولی استئوسارکوم سگ D-17

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: حیوانات خانگی در دنیای مدرن در معرض انبوهی از مواد سرطان‌زا هستند. در نتیجه، نرخ بالایی از سرطان، به ویژه در گربه‌ها و سگ‌های میانسال مشاهده می‎شود. اگرچه بیس‌فسفونات‌ها در درمان سرطان‌های انسانی گنجانده شده‌اند، با این حال مطالعات کمی برای حیوانات وجود دارد. از آنجایی که تعداد موارد سرطان در حیوانات همچنان در حال افزایش است، ارزیابی داروهای ضد سرطان بر اساس گونه‌های خاص اهمیت فزاینده‌ای پیدا می‌کند. هدف: هدف از این مطالعه، بررسی تاثیر زولدرونیک اسید (ZA) بر مسیرهای آپوپتوز در رده‌های سلولی استئوسارکوم سگ (OSA) بود. روش کار: اثرات آپوپتوز تجویز ZA بر روی سلول‌های OSA سگ D-17 با تعیین شکست‌های DNA آپوپتوتیک، سطوح کاسپاز 3، 8 و 9 با روش ELISA و نسبت Bax/Bcl-2 با استفاده از qRT-PCR مورد بررسی قرار گرفت. اثر ZA بر ظرفیت تشکیل کلنی سلول‌ها با روش رنگ آمیزی کریستال ویولت بررسی شد. ظرفیت اتصال مواد معدنی کاربرد ZA در سلول‌ها با تکنیک رنگ آمیزی Alizarin Red S بررسی شد. تغییر در فعالیت آنزیم آلکالین فسفاتاز در سلول‌های OSA ناشی از تیمار ZA با روش رنگ سنجی تعیین شد. اثر ضد متاستاتیک با روش التیام زخم تعیین شد که پتانسیل مهاجرت سلول‌ها را ارزیابی کرد. نتایج: در حالی که کاربرد ZA در 24 ساعت اول اثر سیتوتوکسیک معنی‌داری را در سلول‌ها نشان نداد، بسته به افزایش دوز و زمان، میزان زنده‌مانی سلول‌ها کاهش یافت. غلظت‌های دوز پایین ZA (1، 5، 5/7، 10 میکرومولار) باعث افزایش محتوای معدنی و فعالیت آنزیم آلکالین فسفاتاز شد. کاهش قابل توجهی در سطح بیان سوروایوین یافت شد که بسته به دوز و زمان، بقای سلول را تعیین می‌کند. نتیجه‌گیری: انتظار می‌رود که داده‌های به‌ دست آمده با ایجاد گزینه‌های درمانی مختلف برای پزشکان به واسطه افزایش دانش در زیست شناسی سلول‌های سرطانی، به درمان مؤثرتر این بیماری کمک کند.

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موضوعات


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