پیوند استخوان زنوژنیک (بیگانه زا) بدون سلول برای ترمیم نقص استخوانی سگمنتال در خرگوش

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: پیوند استخوان یک گزینه درمانی ارجح برای بهبود نقایص بزرگ استخوان دیافیز است و در مدیریت جوش نخوردن، جوش خوردن تاخیری و برداشتن تومور مفید است. هدف: بررسی یک روش سلول‌زدایی از استخوان اسفنجی گاو، برای پیوند زنوژنیک در نقایص استخوان رادیال در خرگوش. روش کار: داربست‌های استخوان گاوی با پروتکل‌های سلول‌زدایی مختلف مانند بافر فسفات سالین (PBS)، سدیم دودسیل سولفات 1% (SDS) و روش انجماد و ذوب سریع ساخته شدند. این داربست‌های ساخته شده با آزمایش‌های بیومکانیکی، رنگ آمیزی بافت‌شناسی و میکروسکوپ الکترونی روبشی مورد شناسایی قرار گرفتند. یک نقص 10 میلی متری در استخوان زند زبرین خرگوش به وسیله اتوگرافت و همچنین داربست‌های استخوانی سلول‌زدا شده با SDS، انجماد و ذوب سریع به ترتیب در گروه‌های A، B، و C ترمیم شد. بهبودی با استفاده از رادیوگرافی و هیستوپاتولوژی در روزهای 0، 30، 60، و 90 مورد ارزیابی قرار گرفت. پیوندها همچنین از نظر واکنش بافتی میزبان و ایجاد نقص بررسی شدند. نتایج: گروه انجماد و ذوب حذف کامل تمام هسته‌های سلولی، آرایش منظم فیبر کلاژن و همچنین عدم تفاوت معنی‌داری در استحکام کششی در مقایسه با گروه تیمار شده با SDS 1% و گروه کنترل را نشان دادند. بررسی رادیوگرافیک درون‌تنی و مطالعات هیستوپاتولوژیک نشان داد که گروه انجماد و ذوب سریع، شکاف استخوانی را با تشکیل استخوان جدید پل زدند و از نظر ایمونولوژیک نسبت به گروه B واکنش کمتری داشتند. نتیجه‌گیری: ارزیابی برون‌تنی و درون‌تنی پیوند‌ها نشان داد که روش انجماد و ذوب، از همه تکنیک‌های دیگر برای سلول‌زدایی مؤثر و تقویت ترمیم استخوان با ادغام بهتر پیوند در میزبان، برتر است.

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