پاکسازی توکسین قارچ زیرالنون از شیرابه شکمبه گاو توسط ترکیبات فیتوشیمیایی Zataria multiflora

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: زیرالنون (ZEA) که از رایج‌ترین مایکوتوکسین‌های دانه گندم و ذرت است، باعث مسمومیت حاد و مزمن در دام، طیور و آبزیان می‌شود. در میان جاذب‌های تجاری توکسین، فقط تعداد کمی از کربن‌های فعال توانایی جذب زیرالنون دارند، با این وجود آن‌ها به عنوان یک افزودنی مناسب در صنایع خوراک دام در نظر گرفته نمی‌شوند. هدف: این مطالعه با هدف غربالگری و شناسایی ترکیبات گیاه                Zataria multiflora (آویشن شیرازی) تخریب کننده زیرالنون در شیرابه شکمبه گاو انجام شد. روش کار: اسانس و عصاره‌های مختلف      (ان-هگزانی، اتیل استاتی و متانولی) اندام هوایی آویشن شیرازی (در سه غلظت 5/0، 1، و 2 میلی گرم در میلی لیتر) جهت کاهش میزان زیرالنون (2 میکروگرم در میلی لیتر) در شیرابه شکمبه گاو پایش شدند. محتوای زیرالنون توسط کروماتوگرافی مایع با عملکرد بالا دارای ردیاب فلورسانس (HPLC-FLD) مورد بررسی قرار گرفت. ترکیبات فیتوشیمیایی مسئول پاکسازی زیرالنون، از طریق پروفایل فعالیت مبتنی بر HPLC مکان یابی شدند و سپس توسط طیف سنجی جرمی LC-MS شناسایی شدند. نتایج: عصاره‌های ان-هگزانی و متانولی Z. multiflora به میزان قابل توجهی (78%-63) زیرالنون شیرابه شکمبه را کاهش دادند. با توجه به پروفایل فعالیت Z. multiflora مبتنی بر HPLC و تجزیه و تحلیل طیف سنجی جرمی، دو ترکیب تری‌ترپن شامل اورسولیک اسید و اولئانولیک اسید به عنوان عوامل تخریب کننده زیرالنون شناسایی شدند. نتیجه‌گیری: گیاه Z. multiflora می‌تواند به عنوان منبع گیاهی جدید برای تجزیه زیرالنون توصیه شود و اورسولیک اسید و اولئانولیک اسید به عنوان ترکیبات فیتوشیمیایی جدید برای تخریب زیرالنون معرفی می‌شوند.

کلیدواژه‌ها


Ali, MS; Saleem, M; Ali, Z and Ahmad, VU (2000). Chemistry of Zataria multiflora (lamiaceae). Phytochemistry. 55: 933-936.
Alinezhad, S; Kamalzadeh, A; Shams-Ghahfarokhi, M; Rezaee, MB; Jaimand, K; Kawachi, M; Zamani, Z; Tolouei, R and Razzaghi-Abyaneh, M (2011). Search for novel antifungals from 49 indigenous medicinal plants: Foeniculum vulgare and Platycladus orientalis as strong inhibitors of aflatoxin production by Aspergillus parasiticus. Ann. Microbiol., 61: 673-681.
Bailey, RW (1967). Quantitative studies of ruminant digestion, New Zeal. J. Agr. Res., 10: 15-32.
Belhassen, H; Jiménez-Díaz, I; Arrebola, J; Ghali, R; Ghorbel, H; Olea, N and Hedili, A (2015). Zearalenone and its metabolites in urine and breast cancer risk: A case-control study in Tunisia. Chemosphere. 128: 1-6.
British Pharmacopoeia (1998). HMSO, London. 2: A137-A138.
CAST (2003). Mycotoxins: risks in plant, animal, and human systems. In: Richard, JL and Payne, GA (Eds.), Task Force Report No. 139, Ames, Iowa, USA, The Council for Agricultural Science and Technology. P: 199.
Castillejos, L; Calsamiglia, S and Ferret, A (2006). Effect of essential oil active compounds on rumen microbial fermentation and nutrient flow in in vitro systems. J. Anim. Sci., 89: 2649-2658.
Cho, K; Kang, J; Cho, W; Lee, C; Ha, J and Song, KB (2010). In vitro degradation of zearalenone by Bacillus subtilis. Biotechnol. Lett., 32: 1921-1924.
Cuppett, SL and Hall, C (1998). Antioxidant activity of the labiatae. Adv. Food Nutr. Res., 42: 245-272.
Eftekhar, E; Zamani, S; Yusefzadi, M; Hadian, J and Nejad Ebrahimi, S (2011). Antibacterial activity of Zataria multiflora Boiss. essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumonia. Jundishapur J. Microbiol., 4: 43-49.
El-Sharkaway, S; Selim, MI; Afifi, MS and Halaweish, FT (1991). Microbial transformation of zearalenone to a zearalenone sulfate. Appl. Environ. Microbiol., 57: 549-552.
Fakour, M; Alameh, A; Rasouli, I and Mazaheri, M (2007). Antifungal effects of Zataria multiflora Boiss. and Thymus eriocalyx (ronniger) jalas essential oils on aflatoxin producing Aspergillus parasiticus. Iran. J. Med. Aromat. Plants., 23: 269-277.
Farzaneh, M; Kiani, H; Sharifi, R; Reisi, M and Hadian, J (2015). Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biol. Technol., 109: 145-151.
Gorran, A; Farzaneh, M; Shivazad, M; Rezaeian, M and Ghassempour, A (2013). Aflatoxin b1-reduction of Aspergillus flavus by three medicinal plants (lamiaceae). Food Control. 31: 218-223.
Gupta, G and Gupta, NL (1972). Constituents of Zataria multiflora. Phytochemistry. 11: 455-456.
Hernández-Pérez, M; Rabanal, RM; De La Torre, MC and Rodríguez, B (1995). Analgesic, anti-inflammatory, antipyretic and haematological effects of aethiopinone, an o-naphthoquinone diterpenoid from Salvia aethiopis roots and two hemisynthetic derivatives. Planta. Med., 61: 505-509.
Hoseiniyeh, FS; Mirabolfathy, M; Rezaie, DH and Karami, OR (2012). Effect of five essential oils on zearalenon production and growth of Fusarium graminearum. Appl. Entomol. Phytopathol., 80: 81-94.
Hosseinzadeh, H; Hadad, KMH and Shokouhi, ZH (1998). Antihyperglycemic effect of Salvia lerrifolia benth. Leaf and seed extract in mice. Iran. J. Med. Sci., 23: 74-80.
Huwig, A; Freimund, S; Käppeli, O and Dutler, H (2001). Mycotoxin detoxication of animal feed by different adsorbents. Toxicol. Lett., 122: 179-188.
Kohiyama, CY; Ribeiro, MMY; Mossini, SAG; Bando, E; Da Silva Bomfim, N; Nerilo, SB; Rocha, GHO; Grespan, R; Mikcha, JMG and Machinski Jr, M (2015). Antifungal properties and inhibitory effects upon aflatoxin production of Thymus vulgaris l. by Aspergillus flavus link. Food Chem., 173: 1006-1010.
Krizsan, SJ and Huhtanen, P (2013). Effect of diet composition and incubation time on feed indigestible neutral detergent fiber concentration in dairy cows. J. Dairy Sci., 96: 1715-1726.
Liu, J (1995). Pharmacology of oleanolic acid and ursolic acid. J. Ethnopharmacol., 49: 57-68.
Luo, Y; Yoshizawa, T and Katayama, T (1990). Comparative study on the natural occurrence of Fusarium mycotoxins (trichothecenes and zearalenone) in corn and wheat from high- and low-risk areas for human esophageal cancer in China. Appl. Environ. Microbiol., 56: 3723-3726.
Mohagheghzadeh, A; Shams-Ardakani, M; Ghannadi, A and Minaeian, M (2004). Rosmarinic acid from Zataria multiflora tops and in vitro cultures. Fitoterapia. 75: 315-321.
Peana, A; Satta, M; Moretti, ML and Orecchioni, M (1994). A study on choleretic activity of Salvia desoleana essential oil. Planta. Med., 60: 478-479.
Pietsch, C; Kersten, S; Burkhardt-Holm, P; Valenta, H and Dänicke, S (2013). Occurrence of deoxynivalenol and zearalenone in commercial fish feed: An initial study. Toxins. 5: 184-192.
Pietsch, C; Noser, J; Wettstein, FE and Burkhardt-Holm, P (2014). Unraveling the mechanisms involved in zearalenone-mediated toxicity in permanent fish cell cultures. Toxicon. 88: 44-61.
Rajaei, H and Khajehali, M (2009). Histochemical screening of flavonoids in secretory trichomes of Zataria multiflora leaves. Planta. Med., 9 (57th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research: Geneva, Switzerland), 75: 154.
Ramos, AJ; Fink-Gremmels, J and Hernandez, E (1996). Prevention of toxic effects of mycotoxins by means of nonnutritive adsorbent compounds. J. Food Prot., 59: 631-641.
Reddy, K; Reddy, C and Muralidharan, K (2009). Potential of botanicals and biocontrol agents on growth and aflatoxin production by Aspergillus flavus infecting rice grains. Food Control. 20: 173-178.
Rodrigues, I (2014). A review on the effects of mycotoxins in dairy ruminants. Anim. Prod. Sci., 54: 1155-1165.
Sajed, H; Sahebkar, A and Iranshahi, M (2013). Zataria multiflora Boiss. (Shirazi thyme)—an ancient condiment with modern pharmaceutical uses. J. Ethnopharmacol., 145: 686-698.
Sandosskumar, R; Karthikeyan, M; Mathiyazhagan, S; Mohankumar, M; Chandrasekar, G and Velazhahan, R (2007). Inhibition of Aspergillus flavus growth and detoxification of aflatoxin B1 by the medicinal plant zimmu (Allium sativum L. × Allium cepa L.). World J. Microbiol. Biotechnol., 23: 1007-1014.
Shokri, H and Sharifzadeh, A (2017). Zataria multiflora Boiss.: A review study on chemical composition, anti-fungal and anti-mycotoxin activities, and ultrastructural changes. J. Herbmed. Pharmacol., 6: 1-9.
Shomali, T and Mosleh, N (2019). Zataria multiflora, broiler health and performance: a review. Iran. J. Vet. Res., 20: 81-88.
Soltani, M; Zarifmanesh, T and Zorriehzahra, S (2013). Effect of Zataria multiflora essential oils on rainbow trout (Oncorhynchus mykis) complement component activity and lysozyme. Iran. Sci. Fish. J., 21: 13-23.
Taghavi-Nezhad, M; Alipour, D; Flythe, MD; Zamani, P and Khodakaramian, G (2013). The effect of essential
oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acid-fermenting bacteria isolated from Mehraban sheep. Anim. Prod. Sci., 54: 299-307.
Takahashi-Ando, N; Ohsato, S; Shibata, T; Hamamoto, I; Yamaguchi, I and Kimura, M (2004). Metabolism of zearalenone by genetically modified organisms expressing the detoxification gene from Clonostachys rosea. Appl. Environ. Microb., 70: 3239-3245.
Tanaka, T; Hasegawa, A; Yamamoto, S; Lee, US; Sugiura, Y and Ueno, Y (1988). Worldwide contamination of cereals by the Fusarium mycotoxins nivalenol, deoxynivalenol, and zearalenone. 1. Survey of 19 countries. J. Agric. Food Chem., 36: 979-983.
Taylor, WJ and Draughon, FA (2001). Nannocystis exedens: A potential biocompetitive agent against Aspergillus flavus and Aspergillus parasiticus. J. Food Prot., 64: 1030-1034.
Velazhahan, R; Vijayanandraj, S; Vijayasamundeeswari, A; Paranidharan, V; Samiyappan, R; Iwamoto, T; Friebe, B and Muthukrishnan, S (2010). Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex turrill-structural analysis and biological toxicity of degradation product of aflatoxin G1. Food Control. 21: 719-725.
Ward, T; Watkins, K; Southern, L; Hoyt, P and French, DD (1991). Interactive effects of sodium zeolite-a and copper in growing swine: Growth, and bone and tissue mineral concentrations. J. Anim. Sci., 69: 726-733.
Wasser, S; Ho, JMS; Ang, HK and Tan, CEL (1998). Salvia miltiorrhiza reduces experimentally-induced hepatic fibrosis in rats. J. Hepatol., 29: 760-771.
Yi, PJ; Pai, CK and Liu, JR (2011). Isolation and characterization of a Bacillus licheniformis strain capable of degrading zearalenone. World J. Microbiol. Biotechnol., 27: 1035-1043.
Yu, Y; Qiu, L; Wu, H; Tang, Y; Lai, F and Yu, Y (2011). Oxidation of zearalenone by extracellular enzymes from Acinetobacter sp. SMo4 into smaller estrogenic products. World J. Microbiol. Biotechnol., 27: 2675-2681.