اولین تایید مولکولی-بالینی لیستریا مونوسیتوژنز بیماری‌زا در گاومیش‌های نیلی-راوی

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: لیستریوز یک بیماری مشترک بین انسان، حیوانات، پرندگان، ماهی‌ها، و سخت پوستان در سراسر جهان است. حیوانات اهلی به ویژه نشخوارکنندگان بیشتر به لیستریوز مستعد هستند. این بیماری عفونی توسط لیستریا مونوسیتوژنز ایجاد می‌شود که یک باکتری داخل سلولی است و می‌تواند از سد خونی مغزی، جفت و سد روده‌ای عبور کند. در پاکستان، میزان بروز و همچنین ابزارهای تشخیصی قابل اعتماد برای لیستریا مونوسیتوژنز در گاومیش‌های نیلی-راوی مشخص و شناخته شده نیستند. هدف: این مطالعه به منظور شناسایی لیستریوز در گاومیش‌ها از طریق تکنیک‌های مولکولی و همچنین آنالیزهای هماتو-بیوشیمیایی طراحی شده است. روش کار: در مجموع 230 نمونه (115 نمونه شیر و 115 نمونه مدفوع) از موارد لیستریوز علامت‌دار در گاومیش‌های نیلی-راوی از 3 ناحیه جغرافیایی (راولپندی، فیصل آباد، مظفرگار) پنجاب، کشور پاکستان جمع آوری شد. این نمونه‌ها با استفاده از کیت‌های تجاری تحت استخراج DNA قرار گرفتند. و تایید آلودگی با لیستریا مونوسیتوژنز به وسیله PCR معمولی انجام شد. نتایج: نتایج نشان داد که به ترتیب 08/6%، و 34/4% از جدایه‌های حاصل از نمونه‌های شیر و مدفوع برای لیستریا مونوسیتوژنز مثبت بودند. آنالیز فیلوژنیک این ایزوله‌ها شباهت 97 تا 100% را با ایزوله‌های آمریکا، سوئیس، ژاپن، و هند نشان داد. شماره‌های الحاق در بانک ژنی NCBI، HF558398 (سوئیس)، KP965732 (هند)، EU372032 (آمریکا) و LC259850 (ژاپن) بود. آزمایش‌های هماتو-شیمیایی نشان داد که مقادیر WBC، فیبرینوژن پلاسما، ALT، و AST در گاومیش‌های بیمار در مقایسه با موارد سالم افزایش یافته است (P<0.05). نتیجه‌گیری: رخداد لیستریوز در گاومیش‌ها پایش و نظارت مداوم و پیوسته به منظور جلوگیری از این بیماری در حال تکوین در پاکستان را ضروری می‌سازد.

کلیدواژه‌ها

موضوعات


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