بررسی کیفیت ایمونولوژیک و باکتریولوژیک آغوز تازه گاو و انتقال ایمنی غیر فعال در گاوداری‌های شیری منتخب استان فارس‏، ایران

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: کیفیت آغوز تحت تاثیر دو عامل مهم شامل سطح IgG و بار میکروبی قرار می‌گیرد. هدف: در این مطالعه کیفیت آغوز مورد استفاده در تغذیه گوساله‌های شیری و انتقال ایمنی غیر فعال در گاوداری‌های استان فارس، ایران مورد بررسی قرار گرفت. روش کار: در مجموع 75 نمونه آغوز و خون گوساله‌های نوزاد از 11 گله جمع آوری شد. کیفیت ایمونولوژیک آغوز با استفاده از رفرکتومتر دیجیتال بریکس مورد بررسی قرار گرفت. نمونه‌های آغوز از نظر شمارش باکتریایی تام (TPC)، شمارش کلی فرمی تام (TCC)، شمارش اسپورها و شمارش قارچ‌ها بررسی شد و از روش PCR برای تشخیص برخی گونه‌های باکتریایی استفاده شد. نتایج: میانگین امتیاز بریکس نمونه‌های آغوز 4/25% بود و 72% نمونه‌ها نمره بریکس ≥22% داشتند. میانگین بریکس سرم و شیوع FPT به ترتیب 10 و 4% بود. میانگین TPC، TCC، تعداد اسپورها و تعداد قارچ‌ها به ترتیب 105 × 6/3، 104 × 8/2، 104 × 2/3 و 104 × 1/1 CFU/ml بود. نتایج نشان داد که 50%، 9/5% و 4% از نمونه‌های آغوز به ترتیب از نظر گونه‌های استافیلوکوکوس، گونه‌های سالمونلا و مایکوباکتریوم پاراتوبرکلوزیس مثبت بودند. شواهدی مبنی بر آلودگی با گونه‌های بروسلا، کورینه باکتریوم بویس و مایکوپلاسما بویس وجود نداشت. نتیجه‌گیری: وقتی همه شاخص‌های کیفیت آغوز با هم در نظر گرفته شدند، تنها 3/37% نمونه‌ها مطابق استاندارد بودند. تعداد زیادی گوساله در خطر دریافت آغوز بی‌کیفیت، به ویژه از نظر آلودگی میکروبی هستند. تحقیقات بیشتری برای ارزیابی مدیریت آغوز و تاثیر آلودگی باکتریایی آغوز بر سلامت گوساله‌های نوزاد در این منطقه مورد نیاز است.

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