تغییرات در مورفولوژی سطح، رنگ آمیزی لکتین و بیان ژن اندومتریوم بزی مواجه شده با استرادیول، پروژسترون و میفپریستن در شرایط in vitro

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: در پستانداران ماده بافت رحم به شدت به هورمون‌های استروئیدی و آنتاگونیست‌های آن‌ها پاسخ می‌دهد. هدف: در مطالعه حاضر تغییرات توپوگرافی، بافتی ساختاری و بیان ژن در اندومتر بزی تیمار شده با استرادیول، پروژسترون و میفپریستن به مدت 24 ساعت در شرایط  in vitro مورد بررسی قرار گرفت. روش کار: میکروسکوپ الکترونی روبشی برای ارزیابی توپوگرافی سطح، WGA و DBA لکتین‌ها برای ارزیابی هیستوشیمیایی استفاده شد و qRT-PCR هم برای تایین سطوح mRNA مربوط به MKI67 (نشانگر تکثیر Ki67)، ESR1 (گیرنده استروژن)، PGR (گیرنده پروژسترون)، CASP3 (caspase 3) و PDGFR-β (فاکتور رشد گیرنده بتا مشتق از پلاکت) انجام شد. نتایج: تغییرات توپوگرافی محدودی در غدد آندومتر و همچنین وجود گرانول‌های مخاطی پراکنده مشاهده شد. کاهش معنی‌داری در رنگ آمیزی WGA فقط در گروه پروژسترون مشاهده شد. با این حال، DBA بیشترین اتصال را در گروه پروژسترون و کمترین اتصال را در گروه میفپریستن داشت. بیان ژن MKI67 در گروه میفپریستن 79% کاهش یافت، در حالی که در گروه استرادیول و پروژسترون به ترتیب 153% و 41% نسبت به گروه شاهد افزایش یافت. روند مشابه‌ای برای PDGFR-β مشاهده شد. فراوانی mRNA برای ESR1 در گروه پروژسترون به 59% و در گروه میفپریستن به 10% کاهش یافت. با این حال افزایش 100% در گروه استرادیول اتفاق افتاد. بیان PGR نیز روند مشابه‌ای با ESR1 داشت. بیان CASP3 در گروه استرادیول (50%) و گروه پروژسترون (37%) کاهش یافت، اما در گروه میفپریستن 67% افزایش یافت. نتیجه‌گیری: ما به این نتیجه رسیدیم که رحم بز در پاسخ به انواع مختلف استروئیدها، در ساختار و عملکرد خود دچار تغییرات چشمگیری می‌شود.

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