تاثیر دستاکسل بر بقا، میزان لقاح و بیان mRNA ژن‌های مرتبط با آپوپتوز در تخمک‌های متافاز II موش پس از انجماد شیشه‌ای .

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: دستاکسل در انجماد تخمک مفید است. هدف: اثر دستاکسل بر بقا، میزان لقاح و بیان mRNA ژن‌های مربوط به آپوپتوز در تخمک‌های بالغ منجمد شده بررسی گردید. روش کار: تخمک‌های بالغ به هشت گروه آزمایشی تقسیم شدند شامل 1) کنترل، 2) دستاکسل، 3) دستاکسل + محلول کرایوپروتکتانت 1 (CPA1)، 4) دستاکسل + CPA2، 5) دستاکسل + ویتریفیکاسیون 1 (Vit1)، 6) دستاکسل + Vit2، 7) Vit1، و 8) Vit2. میزان بقا و لقاح و سطح بیان mRNA Bcl-xl، Bax و کاسپاز-3 به عنوان ژن‌های مرتبط با آپوپتوز مورد بررسی قرار گرفت. نتایج: میزان بقا در گروه‌های Vit1 و Vit2 به طور معنی‌داری پایین‌تر از گروه کنترل بود (P<0.05). میزان لقاح در دستاکسل + Vit1، دستاکسل + Vit2، Vit1 و Vit2 به طور معنی‌داری پایین‌تر از گروه کنترل، دستاکسل و گروه‌های مرتبط با استفاده از دستاکسل + CPAs بود. بیان Bax در گروه‌هایی که تخمک‌ها منجمد شده بودند به طور معنی‌داری افزایش یافت. همچنین بیان آن در گروه Vit2 در مقایسه با گروه دستاکسل + Vit2 به طور معنی‌داری افزایش یافت. بیان ژن Bcl-xl در گروه دستاکسل + CPA2، دستاکسل + Vit2 و Vit2 نسبت به گروه دستاکسل به طور معنی‌داری کمتر شد. نسبت Bax/Bcl-xl در گروه‌های دستاکسل + CPA2، دستاکسل + Vit1، دستاکسل + Vit2، گروه Vit1 و Vit2 در مقایسه با گروه کنترل، دستاکسل و دستاکسل + CPA1 به طور معنی‌داری افزایش یافت. بیان کاسپاز-3 در هر شش گروه در مقایسه با گروه‌های کنترل و دستاکسل به طور معنی‌داری افزایش یافت. بیان آن در گروه Vit1 و Vit2 به ترتیب نسبت به دستاکسل + Vit1 و دستاکسل + Vit2 به طور معنی‌داری افزایش یافت. نتیجه‌گیری: دستاکسل با تغییر در بیان ژن‌های مرتبط با آپوپتوز، آسیب‌ به تخمک‌ها را در طول انجماد بهبود می‌بخشد و اثرات آن به محلول انجماد مورد استفاده در انجماد تخمک‌ها وابسته است.

کلیدواژه‌ها

موضوعات


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