Serogroups, and drug resistance of nontyphoidal Salmonella in symptomatic patients with community-acquired diarrhea and chicken meat samples in Tehran

Document Type : Full paper (Original article)


1 MSc in Microbiology, Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran

2 MSc Student in Microbiology, Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 MSc in Microbiology, Division of Microbiology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran

5 Division of Microbiology, Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

6 Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

7 Division of Microbiology, Anti-Microbial Resistance Research Center, Institute of Immunology and Infectious Diseases (IIID), Iran University of Medical Sciences, Tehran, Iran

8 MSc in Microbiology, Division of Microbiology, Anti-Microbial Resistance Research Center, Institute of Immunology and Infectious Diseases (IIID), Iran University of Medical Sciences, Tehran, Iran

9 Health Reference Laboratory, Health Reference Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran

10 MSc in Microbiology, Department of Microbiology, Central Laboratory, Milad Hospital, Tehran, Iran

11 Pathology Laboratory, Mehr Hospital, Tehran, Iran

12 Center for Communicable Disease Control, Ministry of Health and Medical Education, Tehran, Iran

13 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

14 Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran


Background: Salmonella is considered as a main cause of community-acquired diarrhea in humans, however, sources of the multi-drug resistant (MDR) strains and their link with the disease are not well known. Aims: This study aimed to investigate the frequency, serogroup diversity, and antimicrobial susceptibility patterns of Salmonella strains in poultry meat and stool samples of patients with community acquired diarrhea in Tehran. Methods: We compared the frequency of non-typhoidal Salmonella serogroups, the similarities of their resistance patterns to 10 antimicrobial compounds, the prevalence of extended spectrum β-lactamase (ESBL) and ampicillinase C (AmpC) genetic determinants, and class 1 and 2 integrons in 100 chicken meat and 400 stool samples of symptomatic patients in Tehran during June 2018 to March 2019. Results: Salmonella was isolated from 75% and 5.5% of the chicken meats and human stool samples, respectively. The chicken meat isolates mainly belonged to serogroup C (88%, 66/75), while the human stool isolates were mainly related to serogroup D (59.1%, 13/22). The MDR phenotype and the most common rates of resistance to antibiotics, including tetracycline, trimethoprim/sulfamethoxazole (TS) and azithromycin, were detected in 4.5% and 45.3%, 59% and 13.6%, 43% and 9.1%, 42% and 9.1% of the human stool and chicken meat samples, respectively. Carriage of blaCTX, blaSHV, and blaPER genes in the meat isolate with ESBL resistance phenotype and blaACC, blaFOX, and blaCMY-2 among the 7 meat strains with AmpC resistance phenotype was not confirmed using polymerase chain reaction (PCR). High prevalence of class 1 and 2 integrons was characterized and showed a correlation with resistance to TS and chloramphenicol. Conclusion: These findings showed a lack of association between chicken meats and human isolates due to discrepancy between the characterized serogroups and resistance phenotypes.


Main Subjects

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