ویژگی‌های مولکولی آستروویروس، وسیویروس و سیرکوویروس سگ، جدا شده از سگ‌های دچار اسهال در ترکیه

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: آستروویروس سگ (CAstV) به عنوان علت اولیه گاستروانتریت در حیوانات جوان در سراسر جهان در نظر گرفته شده است، در حالی که وسیویروس سگ (CVeV)، و سیرکوویروس سگ (CCiV) گهگاه گزارش می‌شود. هدف: هدف این مطالعه بررسی وجود و خصوصیات مولکولی این 3 ویروس در ترکیه بود. روش کار: نمونه‌های مدفوع از 150 قلاده سگ پناه داده شده دارای مشکلات گوارشی (127 قلاده سگ بالغ و 23 توله) جمع آوری شد و به وسیله آنالیز نسخه‌برداری معکوس-واکنش زنجیره‌ای پلیمراز (RT-PCR) روی توالی بخشی ژن RdRp (ORF1b) برای CAstV، ژن ORF2 از CVeV و پروتئین کپسید (Cap) و ژن پروتئین مرتبط با همانندسازی (Rep) از CCiV مورد بررسی قرار گرفتند. نمونه‌های مثبت به صورت تصادفی انتخاب شده و جهت توالی یابی ارسال شدند و آنالیز مولکولی بر اساس توالی یابی بخشی انجام شد. نتایج: مشخص شد که 66% (99 از 150) از سگ‌های مبتلا به اسهال برای CAstV، 33/3% (5 از 150) برای CVeV و 6% (9 از 150) برای CCiV مثبت بودند. چهار تحت ژنوتیپ برای CAstV و 2 تحت ژنوتیپ برای CVeV بر اساس آنالیز مولکولی پیشنهاد می‌شود. رابطه فیلوژنیک CCiV با سویه‌های دیگر به دست آمده از مناطق مختلف بیشتر نشان داده شد. نتیجه‌گیری: این مطالعه بر اهمیت ویروس‌های نوظهور در سگ سانان، طبقه بندی آن‌ها و سهم متناسب این ویروس‌ها در موارد گاستروانتریت تاکید دارد. نتیجه گرفتیم که عفونت آستروویروسی به عنوان مهمترین عامل اسهال در سگ‌ها باید مورد توجه قرار گیرد. با این وجود شیوع وسیوویروس و سیرکوویروس در موارد نسبتا کم، باعث می‌شود که از اهمیت کمتری در ترکیه برخوردار باشند.

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