آنالیز فیلوژنتیک ژن بخشی توالی یابی شده cytb هموپروتئوس کلمبه در کبوتران و ضایعات پاتولوژیک ناشی از آن‌ در مصر

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: هموپروتئوس کلمبه به طور گسترده‌ای در مناطق گرم سیری و نیمه گرم سیری پراکنده شده است و سبب ایجاد بیماری شبه مالاریا در کبوتران می‌شود. هدف: مطالعه حاضر با هدف توصیف موقعیت فیلوژنیک هموپروتئوس کلمبه در کبوتران در استان شارکیا مصر بر اساس توالی یابی بخشی ژن cytb به عنوان مناطق حفاظت شده، انجام شده است. بارکد DNA از ژن cytb در طراحی ‎آغازگرهایی که می‌توانند برای تکثیر ژن مشابه در سایر هوموسپوریدین‌های مرتبط استفاده شوند، کمک می‌کند. روش کار: یکصد نمونه خون از کبوتران اهلی برای شناسایی هموپروتئوس کلمبه به وسیله واکنش زنجیره‌ای پلیمراز (PCR) و بررسی رابطه آن با سایر هوموسپوریدین‌ها گرفته شد. نتایج: در پرندگان مورد مطالعه، کاهش وزن 60%، آنمی 40%، نرخ رشد پایین 67/26%، اسهال 67/76%، تنگی نفس 67/66%، برخی علائم عصبی 33/33% و مرگ و میر 67/16% مشاهده شد. در معاینه پس از مرگ نمای قهوه‌ای-شکلاتی در کبد پرندگان و احتقان پارانشیم ارگان‌ها مشاهده شد. در بررسی ریزسنجی اسمیرهای خون (n=100) رنگ آمیزی شده با گیمسا، عفونت به میزان 30% مشاهده شد. درصد عفونت مشاهده شده در نرها (71/35%) نسبت به ماده‌ها (66/16%) بارزتر و در بالغین (14/57%) نسبت به کبوترهای جوان (38/15%) بیشتر بود. توالی مورد نظر مربوط به هموپروتئوس کلمبه در بانک ژن با شماره MH345964 ثبت شد و با سایر سویه‌های هموپروتئوس در باغ وحش سائو پائولو برزیل KU131585) و (KU131583 و انگلستان KX832586) و (KX832586 100% تطابق داشت. نتیجه‌گیری: بر اساس نتایج این مطالعه تشخیص دقیق عفونت هموپروتئوس کلمبه در کبوترها با استفاده از آغازگرهای اختصاصی به درمان زود هنگام موارد مبتلا، به خصوص در حضور اشکال نابالغ کمک خواهد کرد و در نتیجه می‌تواند از نشانه‌های بالینی و ضایعات پاتولوژیک ناشی از آن که در این مطالعه ذکر شد، جلوگیری کند.

کلیدواژه‌ها

موضوعات


Abdul Momin, M; Begum, N; Dey, AR; Paran, MS and Alam, MZ (2014). Prevalence of blood protozoa in poultry in Tangail, Bangladesh. J. Agri. Vet. Sci., 7: 55-60.
Abed, AA; Naji, HA and Rhyaf, AG (2014). Investigation study of some parasites infected domestic pigeon (Columba livia domestica) in Al-Dewaniya city. J. Pharm. Bio. Sci., 9: 13-20.
Ahmed, FE and Mohammed, AH (1978). Haemoproteus columbae: course of infection relapse and immunity to reinfection in the pigeon. Z. Parasitenkd., 57: 229-236.
Al-Barwari, S and Saeed, I (2012). The parasitic communities of the rock pigeon Columbae livia from Iraq: component and importance. Turkiye. Parazitol. Derg., 36: 232-239.
Altschul, SF; Gish, W; Miller, W; Myers, EW and Lipman, DJ (1990). Basic local alignment search tool. J. Mol. Bio., 215: 403-410.
Bensch, S; Hellgren, O and Pérez-Tris, J (2009). MalAvi: a public database of malaria parasites and related haemosporidians in avian hosts based on mitochondrial cytochrome b lineages. Mol. Ecol. Resour., 9: 1353-1358.
Borji, H; Moghaddas, E; Razmi, GR and Azad, M (2012). A survey of ecto- and endo-parasites of domestic pigeons (Columba livia) in Mashhad, Iran. Iran. J. Vet. Sci. Techn., 4: 37-42.
Borji, H; Moghaddas, E; Razmi, GR; Bami, MH; Mohri, M and Azad, M (2011). Prevalence of pigeon haemosporidians and effect of infection on biochemical factors in Iran. J. Parasit. Dis., 2: 199-201.
Borkataki, S; Katoch, R; Goswami, P; Godara, R; Khajuria, JK; Yadav, A; Kour, R and Mir, I (2015). Incidence of Haemoproteus columbae in pigeons of Jammu district. J. Parasit. Dis., 39: 426-428.
Cepeda, AS; Lotta-Arévalo, IA; Pinto-Osorio, DF; Macías-Zacipa, J; Valkiūnas, G; Barato, P and Matta, NE (2019). Experimental characterization of the complete life cycle of Haemoproteus columbae, with a description of a natural host-parasite system used to study this infection. Int. J. Parasitol., 49: 975-984.
Chagas, CRF; Guimaraes, LO; Monteiro, EF; Valkiunas, G; Katayama, MV; Santos, SV; Guida, FJ; Simoes, RF and Kirchgatter, K (2016). Hemosporidian parasites of free-living birds in the Sao Paulo Zoo, Brazil. Parasitol. Res., 115: 1443-1452.
Coral, AA; Valkiunas, G; González, AD and Matta, NE (2015). In vitro development of Haemoproteus columbae (Haemosporida: Haemoproteidae), with perspectives for genomic studies of avian haemosporidian parasites. Exp. Parasitol., 157: 163-169. https://doi.org/10.1016/j.exppara. 2015.08.003.
Cottin, V; Donsbeck, AV; Revel, D; Loire, R and Cordier, JF (1998). Nonspecific interstitial pneumonia. Individualization of a clinicopathologic entity in a series of 12 patients. Am. J. Respir. Crit. Care Med., 158: 1286-1293.
Dadi-Mamud, NJ; Kabir, MA; Dibal, DM and Rajab, MH (2012). Study on the prevalence of Haemoparasites of pigeon (Columba livia) in Lapai-Nigeria. Inter. J. Applied Bio. Res., 4: 121-127.
Dey, AR; Begum, N; Paul, SC; Noor, M and Islam, KM (2010). Prevalence and pathology of blood protozoa in pigeons reared at Mymensingh district, Bangladesh. Int. J. Bio. Res., 2: 25-29.
Doosti, A; Ahmadi, R; Mohammadalipour, Z and Zohoor, A (2014). Detection of Haemoproteus columbae in Iranian pigeons using PCR. Paper Presented at the International Conference on Biological, Civil and Environmental Engineering, Dubai (UAE).
Dranzoa, C; Ocaido, M and Katete, P (1999). The ectogastro-intestinal and haemo-parasitesof live pigeons (Columba livia) in Kampala, Uganda. Avian Pathol., 28: 119-124.
Earle, RA and Little, RM (1993). Haematozoa of feral rock doves and rock pigeons in mixed flocks. S. Afr. J. Wildl. Res., 23: 98-100.
Friend, M and Franson, J (1999). Parasitic diseases. In: Field manual of wildlife diseases: general field procedures and diseases of birds. (1st Edn.), Reston, US Geological Survey. P: 425.
Gicik, Y and Arslan, M (2001). Blood parasites of wild pigeons in Ankara District. Turk. J. Vet. Anim. Sci., 25: 169-172.
Gulander, A; Tuzer, E and Cetinkaya, H (2002). Haemoproteus columbae infections and Pseudolynchia canariensis infestations in pigeons in Istanbul, Turkey. Istanbul Univ. Vet. Fak. Derg. Istanb., 28: 227-229.
Gupta, DK; Jahan, N and Gupta, N (2011). New records of Haemoproteus and Plasmodium (Sporozoa: Haemosporida) of rock pigeon (Columba livia) in India. J. Parasit. Dis., 35: 155-168.
Hellgren, O; Waldenström, J and Bensch, S (2004). A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood. J. Parasitol., 90: 797-802.
Hussein, NM and Abdelrahim, EA (2016). Haemoproteus columbae infection and its histopathological effects on pigeons in Qena governorate, Egypt. J. Pharm. Bio. Sci., 11: 79-90.
Islam, MS; Abdul Alim, M; Das, S; Ghosh, KK; Pervin, S; Lipi, A; Siddiki, AZ; Masuduzzaman, M and Hossain, MA (2014). Prevalence of Haemoproteus sp in domestic pigeon at Chittagong and Khulna District in Bangladesh. J. Adv. Parasitol., 1: 24-26.
Jones, MP (2006). Selected infectious diseases of birds of prey. J. Exot. Pet. Med., 15: 5-17.
Joshi, V; Dimri, U; Alam, S and Gopalakrishnan, A (2017). Buparvaquone therapy in a rock pigeon infected with Haemoproteus columbae showing torticollis. J. Parasit. Di., 41: 514-516.
Lane, EW (2003). An account of the manners and of the customs modern Egyptian. 5th Edn., Egypt, American University in Cairo Press. P: 664.
Maharana, BR and Kumar, B (2017). Pseudomalaria in a domestic pigeon: acase report. J. Parasit. Dis., 41: 295-297.
Martinez-Moreno, FJ; Martinez-Mareno, A; Becerra-Martell, C and Martinez-Cruz, MS (1989). Parasitic fauna of pigeons (Columba livia) in Cordoba Province, Spain. Rev. Iber. Parasitol., 49: 279-281.
Msoffe, PLM; Muhairwa, AP and Chiwanga, GH (2010). A study of ecto- and endoparasites of domestic pigeon in Morogoro Municipality. Tanz. Afr. J. Agri. Res., 5: 264-267.
Mubarak, M and Abed, GH (2005). Pathological changes of lung tissues of pigeons (Columba livia domestica) infected with Haemoproteus columbae (Haemosporina: Haemoproteidae). J. Bio. Sci., 5: 536-541.
Mushi, EZ; Binta, MG; Chabo, RG; Mathaio, M and Ndebele, RT (1999). Haemoproteus columbae in domestic pigeons in Sebele, Gaborone, Botswana. Onderstepoort J. Vet. Res., 66: 29-32.
Mushi, EZ; Binta, MG; Chaba, NRG and Panzirah, RR (2000). Parasites of domestic pigeon (Columbia livia domestica) in Sebele, Gaborone, Botswana. J. S. Afr. Vet. Assoc., 7: 249-250.
Natala, AJ; Asemadahun, ND; Okubanjo, OO; Ulayi, BM; Owolabi, YH; Jato, ID and Yusuf, KH (2009) A survey of parasites of domesticated pigeon (Columba livia domestica) in Zaria, Nigeria. Int. J. Soft. Comput., 4: 148-150.
Nematollahi, A; Ebrahimi, M; Ahmadi, A and Himan, M (2012). Prevalence of Haemoproteus columbae and Trichomonas gallinae in pigeons (Columba domestica) in Isfahan, Iran. J. Parasit. Dis., 36: 141-142.
Olias, P; Wegelin, M; Zenker, W; Freter, S; Gruber, AD and Klopfleisch, R (2011). Avian malaria deaths in parrots, Europe. Emerg. Infect. Dis., 17: 950. https://doi.org/10.3201/eid1705.101618.
Ortiz-Catedral, L; Brunton, D; Stidworthy, MF; Elsheikha, HM; Pennycott, T; Schulze, C; Braun, M; Wink, M; Gerlach, H and Pendl, H (2019). Haemoproteus minutus is highly virulent for Australasian and South American parrots. Parasit. Vect., 12: 40. https://doi.org/10.1186/ s13071-018-3255-0.
Pacheco, MA; Battistuzzi, FU; Junge, RE; Cornejo, OE; Williams, CV; Landau, I; Rabetafika, L; Snounou, G; Jones-Engel, L and Escalante, AA (2011). Timing the origin of humanmalarias: the lemurpuzzle. BMC Evolution. Biol., 11: 299. doi: 10.1186/1471-2148-11-299.
Razmi, G and Andalibian, E (2006). Survey on infestation to Haemoproteus columbae in pigeons of Mashhad. Pagh. Saza., 71: 95-96.
Rupiper, DJ (1998). Diseases that affect race performance of homing pigeons. Part II: Bacterial, fungal, and parasitic diseases. J. Avian Med. Surg., 12: 138-148.
Saikia, M; Bhattacharjee, K; Sarmah, PC; Deka, DK; Tamuly, S; Kakati1, P and Konch, P (2019). Prevalence and molecular detection of blood protozoa in domestic pigeon. Int. J. Curr. Microbiol. App. Sci., 8: 1426-1436.
Samani, AA; Kheirabadi, KP and Samani, AD (2013). Prevalence and rate of parasitemia of Haemoproteus columbae in Columba Livia domestica in Southwest of Iran. Iran. J. Parasitol., 8: 641-644.
Scaglione, FE; Pregel, P; Cannizzo, FT; Pérez-Rodríguez, AD; Ferroglio, E and Bollo, E (2015). Prevalence of new and known species of haemoparasites in feral pigeons in northwest Italy. Malar. J., 14: 1-5.
Senlik, B; Gulegen, E and Akyol, V (2005). Prevalence and intensity of Haemoproteus columbae in domestic pigeons. Indian Vet. J., 82: 998-999.
Soulsby, EJL (1982). Helminths, arthropods and protozoa of domesticated animals. 7th Edn., London, Baillière Tindall. P: 215.
Springer, WT (1972). Blood and tissue protozoa. In: Hofstad, MS (Ed.), Diseases of poultry. (6th Edn.), Ames, Iowa State University Press. P: 102.
Suvarna, SK; Layton, C and Bancroft, JD (2013). Bancroft’s theory and practice of histological techniques. 7th Edn., England, Churchill Livingstone, Elsevier. P: 85.
Tamura, K; Stecher, G; Peterson, D; Filipski, A and Kumar, S (2013). Mega 6: molecular evolutionary genetics analysis version 6.0. Mol. Bio. Evol., 30: 2725-2729.
Taylor, MA; Coop, RL and Wall, RL (2007). Veterinary parasitology. 3rd Edn., UK, Blackwell Publishing Ltd., P: 189.
Thompson, JD; Higgins, DG and Gibson, TJ (1994). Clustal
W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22: 4673-4680.
Varshney, JP; Deshmukh, VV and Chaudhary, PS (2014). Pseudomalaria (Haemoproteus columbae) in pigeon shelter. Intas Polivet., 15: 176-177.
Youssefi, MR and Rahimi, MT (2011). Haemoproteus columbae in Columba livia domestica of Three Areas in Iran in 2010. Glob.Vet., 7: 593-595.
Youssefi, MR; Sadeghian, AG and Esfandiari, B (2010). Prevalence of H. columbae infection in Columbia livia in North of Iran. World J. Zoo., 5: 275-277.
Yunus, G and Arsalan, M (2001). Blood parasites of wild pigeons in Ankara. J. Vet. Anim. Sci., 25: 169-172.
Zahan, T; Hossain, S; Dey, AD; Shahiduzzaman,M and Alam, MZ (2018). Prevalence and seasonal distribution of haemosporidian parasites in pigeons of My-mensingh and Rangpur districts, Bangladesh. Eurasian J. Vet. Sci., 34: 117-122.