Fatty acid profile of ewe’s milk infected with Staphylococcus spp.

Document Type : Short paper


1 Department of Public Health, Sumy State University, Rymskogo-Korsakova 2, Sumy 40007, Ukraine

2 Department of Biostructure and Animal Physiology, The Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Norwida 31, Wrocław 50-375, Poland

3 Department of Cattle Breeding and Milk Production, Institute of Animal Breeding, Wrocław University of Environmental and Life Sciences, Chełmońskiego 38c, 51-630 Wrocław, Poland

4 Department of Animal Breeding, Institute of Nutrition, Dietetics and Feed Production, University of Veterinary Medicine and Pharmacy, Komenskeho 73, Kosice 041 81, Slovak Republic


Background: Mastitis in sheep caused by Staphylococcus spp. is a serious concern for dairy farming. Aims: The objective of this study was to evaluate the impact of the intramammary infection (IMI) caused by Staphylococcus spp. on the long-chain fatty acid profile and composition of ewe’s milk. Methods: The experiment was conducted in a herd of Zoslachtena Valaska sheep. Half-udder milk samples were collected from 20 weight-matched sheep at the peak of their first or second lactation. The basic physicochemical composition of milk, somatic cell count (SCC), Staphylococcus spp. infection, and total bacterial contamination (TBC) were determined. The fatty acid profile of the milk fat was determined using gas chromatography. Results: The SCC in milk infected with Staphylococcus spp. was 3.25 times higher (P<0.01) than that in the uninfected milk samples. The content of lauric acid (С12:0) was higher (P<0.05) in the milk fat of infected ewes. A significant increase (P<0.05) in the share of linoleic acid (C18:2n6t), arachidonic acid (C20:4n6), and a decrease (P<0.01) in the vaccenic acid (C18:1n7t) were observed in the milk collected from ewes infected with Staphylococcus spp.. Staphylococcus spp. infection increased the ratio of n-6 to n-3 polyunsaturated fatty acids. Conclusion: Changes in the fatty acid profile of milk caused by Staphylococcus spp. infection decrease the value of ewes’ milk as a health-promoting product.


Main Subjects

Abbondio, M; Fois, I; Longheu, C; Azara, E and Tola, S (2019). Biofilm production, quorum sensing system and analysis of virulence factors of Staphylococcus epidermidis collected from sheep milk samples. Small Rumin Res., 174: 83-87.
Chang, L; Yang, Z; Wu, H; Chen, Y; Shi, X; Mao, Y; Cen, N; Liang, X and Yin, Z (2011). Comparative study on fatty acid composition between normal milk and subclinical mastitis milk of dairy cow. Acta Vet. Zoo. Sinica. 42: 44-47.
Cividini, A and Simčič, M (2015). Poljoprivreda fatty acid profile in milk of Bovec sheep fed in the stable or grazed in different pastures. Agriculture. 21: 109-112.
Dore, S; Liciardi, M; Amatiste, S; Bergagna, S; Bolzoni, G; Caligiuri, V; Cerrone, A; Farina, G; Montagna, CO; Saletti, MA; Scatassa, ML; Sotgiu, G and Cannas, EA (2016). Survey on small ruminant bacterial mastitis in Italy, 2013-2014. Small Rumin Res., 141: 91-93.
Endo, Y; Kamisada, S; Fujimoto, K and Saito, T (2006). Trans fatty acids promote the growth of some Lactobacillus strains. J. Gen. Appl. Microbiol., 52: 29-35.
Fragkou, IA; Boscos, CM and Fthenakis, GC (2014). Diagnosis of clinical or subclinical mastitis in ewes. Small Rumin. Res., 118: 86-92.
Fragkou, IA; Skoufos, J; Cripps, PJ; Kyriazakis, I; Papaioannou, N; Boscos, CM; Tzora, A and Fthenakis, GC (2007). Differences in susceptibility to Mannheimia haemolytica-associated mastitis between two breeds of dairy sheep. J. Dairy Res., 74: 349-355.
Fujita, Y; Matsuoka, H and Hirooka, K (2007). Regulation of fatty acid metabolism in bacteria. Mol. Microbiol., 66: 829-839.
Guetouache, M; Guessas, B and Medjekal, S (2014). Composition and nutritional value of raw milk (Review). Issues Biol. Sci. Pharm. Res., 2: 115-122.
Itoh, T; Fujimoto, Y; Kawai, Y; Toba, T andSaito, T (1995). Inhibition of food-borne pathogenic bacteria by bacteriocins from Lactobacillus gasseri. Lett. Appl. Microbiol., 21: 137-141.
Kelsey, JA; Bayles, KW; Shafii, B and McGuire, MA (2006). Fatty acids and monoacylglycerols inhibit growth of Staphylococcus aureus. Lipids. 41: 951-961.
LeMarechal, C; Thiéry, R; Vautor, E and Le Loir, Y (2011). Mastitis impact on technological properties of milk and quality of milk products - A review. Dairy Sci. Technol., 91: 247-282.
Lu, T; Park, JY; Parnell, K; Fox, LA and McGuire, MA (2012). Characterization of fatty acid modifying enzyme activity in staphylococcal mastitis isolates and other bacteria. BMC Res. Notes. 5: 323-334.
Markiewicz-Keszyńska, M; Czyżak-Runowska, G; Lipińska, P and Wójtowski, J (2013). Fatty acid profile of milk-Review. Bull. Vet. Inst. Pulawy. 57: 135-139.
Mensink, RP; Temme, EHM and Hornstra, G (2009). Dietary saturated and transfatty acids and lipoprotein metabolism. Ann. Med., 6: 461-464.
Merz, A; Stephan, R and Johler, S (2016). Staphylococcus aureus isolates from goat and sheep milk seem to be closely related and differ from isolates detected from bovine milk. Front. Microbiol., 7: 319-325.
Moossavi, S; Atakora, F; Miliku, K; Sepehri, S; Robertson, B; Duan, Q; Becker, AB; Mandhane, PJ; Turvey, SE; Moraes, TJ; Lefebvre, DL; Sears, MR; Subbarao, P; Field, CJ; Bode, L; Khafipour, E and Azad, MB (2019). Integrated analysis of human milk microbiota with oligosaccharides and fatty acids in the child cohort. Front. Nutr., 6: 58-73.
Mortensen, JE; Shryock, TR and Kapral, FA (1992). Modification of bacterial fatty acids by an enzyme Staphylococcus aureus. J. Med. Microbiol., 36: 293-298.
Nazari, R; Godarzi, H; Rahimi Baghi, F and Moeinrad, M (2014). Enterotoxin gene profiles among Staphylococcus aureus isolated from raw milk. Iran. J. Vet. Res., 15: 409-412.
Nudda, A; Battacone, G; Boaventura Neto, O; Cannas, A; Francesconi, AHD; Atzori, AS and Pulina, P (2014). Feeding strategies to design the fatty acid profile of sheep milk and cheese. R. Bras. Zootec., 43: 445-456.
Park, YW; Juárez, M; Ramos, M and Haenlein, GFW (2007). Physico-chemical characteristics of goat and sheep milk. Small Rumin Res., 68: 88-113.
Patterson, E; Wall, R;Fitzgerald, GF; Ross, RP and Stanton, C (2012). Health implications of high dietary omega-6 polyunsaturated fatty acids. J. Nutr. Metab., 2012: 539426-539441.
Pecka-Kiełb, E; Vasil, M; Zachwieja, A; Zawadzki, W;
Elečko, J; Zigo, F; Illek, JandFarkašova, Z (2016). An effect of mammary gland infection caused by Streptococcus uberis on compositionand physicochemical changes of cows’ milk. Polish J. Vet. Sci., 19: 49-55.
Ptáček, M;Milerski, M;Ducháček, J;Schmidová, J;Tančin, V;Uhrinčat, M;Stádník, L andMichlová, T (2019). Analysis of fatty acid profile in milk fat of Wallachian sheep during lactation. J. Dairy Res., 86: 233-237.
Raynal-Ljutovac, K; Pirisi, A; de Crémoux, R and Gonzalo, C (2007). Somatic cells of goat and sheep milk: Analytical, sanitary, productive and technological aspects. Small Rumin Res., 68: 126-144.
Takano, PV; Scapini, VADC; Valentini, T; Girardini, LK; de Souza, FN; Della Libera, AMMP; Heinemann, MB; Chande, CG; Cortez, A; Collet, SG; Diniz, SA and Blagitz, MG (2018). Milk cellularity and intramammary infections in primiparous and multiparous Lacaune ewes during early lactation. Small Rum. Res., 167: 117-122.
Vasil, M; Pecka-Kiełb of, E; Elečko, J; Zachwieja, A; Zawadzki, W; Zigo, F; Illek, J and Farkašova, Z (2016). Effects of udder infections with Staphylococcus xylosus and Staphylococcus warneri on the composition and physicochemical changes in cow’s milk. Polish J. Vet. Sci., 19: 841-848.
Vasileiou, NGC; Chatzopoulos, DC; Gougoulis, DA; Sarrou, S; Katsafadou, AI; Spyrou, V; Mavrogianni, VS; Petinaki, E and Fthenakis, GC (2018). Slime-producing staphylococci as causal agents of subclinical mastitis in sheep. Vet. Micr., 224: 93-99.