تغییرات در بیان mRNA، TLRs و CD14 سلول‌های تک هسته‌ای خون محیطی در متریت بعد از زایمان در گاوهای شیری

نوع مقاله : مقاله کامل

نویسندگان

چکیده

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پیشینه: سلول‌های تک هسته‌ای خون محیطی (PBMCs)، که به طور معمول به مونوسیت‌ها و لنفوسیت‌ها اطلاق می‌شوند، سلول‌های سیستم ایمنی ذاتی و اکتسابی را تشکیل می‌دهند. هدف: پیدا کردن ارتباط بین تغییرات در بیان mRNA گیرنده‌های تشخیص الگو (PRRs) در سلول‌های تک هسته‌ای خون محیطی با متریت بعد از زایمان در گاو هولشتاین. روش کار: سلول‌های تک هسته‌ای خون محیطی از 20 راس گاو دارای متریت بعد از زایمان و 20 راس گاو فاقد متریت در طی 10 روز بعد از زایمان گرفته شدند. بیان ژن‌های گیرنده‌های شبه تول، 2 و 4 TLR2) و (TLR4، و مجموعه تمایزی 14 (CD14) با استفاده از آزمون کمی واکنش زنجیره‌ای پلیمراز زمان حقیقی (RT-PCR) در PBMCs مورد بررسی و ارزیابی قرار گرفت. داده‌ها با ژن گلیسرآلدئید-3-فسفات دی هیدروژناز (GAPDH) به عنوان ژن مرجع نرمال سازی شده و بر اساس روش Ct∆∆-2 مورد ارزیابی قرار گرفت. نتایج: نتایج مطالعه حاضر نشان داد که بیان TLR4 (P=0.04) و CD14 (P=0.008) در گاوهای مبتلا به متریت بعد از زایمان در مقایسه با گروه کنترل به طور معنی‌داری بالاتر بود. با این وجود، بیان TLR2 (P=0.06) در گاوهای مبتلا به متریت بعد از زایمان در مقایسه با گاوهای سالم تفاوت آماری معنی‌داری نداشت. نتیجه‌گیری: این مطالعه پیشنهاد می‌کند که متریت بعد از زایمان به طور معنی‌داری بیان ژن‌های TLR4 و CD14 را در سلول‌های تک هسته‌ای خون محیطی افزایش می‌دهد که به فعالیت مناسب التهاب و پاک سازی باکتریایی رحم بلافاصله بعد از زایمان کمک می‌کند.

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موضوعات


Ahmadi, M; Makki, M; Mirzaei, A and Gheisari, H (2019). Effects of hypertonic dextrose and paraffin solution as non- antibiotic treatments of clinical endometritis on reproductive performance of high producing dairy cows. Reprod. Domest. Anim., 54: 762-771.
Bilal, MS; Abaker, JA; Aabdin, ZU; Xu, T; Dai, H; Zhang, K; Liu, X and Shen, X (2016). Lipopolysaccharide derived from the digestive tract triggers an inflammatory response in the uterus of mid-lactating dairy cows during SARA. BMC Vet. Res., 12: 284-293.
Brubaker, SW; Bonham, KS; Zanoni, I and Kagan, JC (2015). Innate immune pattern recognition: a cell biological perspective. Annu. Rev. Immunol., 33: 257-290.
Cai, TQ; Weston, P; Lund, L; Brodie, B; McKenna, D and Wagner, W (1994). Association between neutrophil functions and periparturient disorders in cows. Am. J. Vet. Res., 55: 934-943.
Cunha, F; Jeon, SJ; Daetz, R; Vieira-Neto, A; Laporta, J; Jeong, KC; Barbet, AF; Risco, CA and Galvao, KN (2018). Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows. Theriogenology. 114: 25-33.
da Mata Martins, T; da Paixão, TA; Costa, ÉA; de Carvalho Pires, A; Santos, RL and Borges, ÁM (2011). Postpartum toll-like receptors and β-defensin 5 mRNA levels in the endometrium of Holstein cows. Vet. Immunol. Immunopathol., 139: 277-281.
Davies, D; Meade, KG; Herath, S; Eckersall, PD; Gonzalez, D; White, JO; Conlan, RS; O’Farrelly, C and Sheldon, IM (2008). Toll-like receptor and antimicrobial peptide expression in the bovine endometrium. Reprod. Biol. Endocrinol., 6: 53.
Eckel, EF and Ametaj, BN (2016). Role of bacterial endotoxins in the etiopathogenesis of periparturient diseases of transition dairy cows. J. Dairy Sci., 99: 5967-5990.
Fischer, C; Drillich, M; Odau, S; Heuwieser, W; Einspanier, R and Gabler, C (2010). Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reprod. Fertil. Dev., 22: 818-829.
Fu, Y; Liu, B; Feng, X; Liu, Z; Liang, D; Li, F; Li, D; Cao, Y; Feng, S and Zhang, X (2013). Lipopolysaccharide increases toll-like receptor 4 and downstream toll-like receptor signaling molecules expression in bovine endometrial epithelial cells. Vet. Immunol. Immunopathol., 151: 20-27.
Herath, S; Dobson, H; Bryant, C and Sheldon, I (2006a). Use of the cow as a large animal model of uterine infection and immunity. J. Reprod. Immunol., 69: 13-22.
Herath, S; Fischer, DP; Werling, D; Williams, EJ; Lilly, ST; Dobson, H; Bryant, CE and Sheldon, IM (2006b). Expression and function of toll-like receptor 4 in the endometrial cells of the uterus. Endocrinology. 147: 562-570.
Herath, S; Lilly, ST; Santos, NR; Gilbert, RO; Goetze, L; Bryant, CE; White, JO; Cronin, J and Sheldon, IM (2009). Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility. Reprod. Biol. Endocrinol., 7: 55-88.
Kawai, T and Akira, S (2010). The rule of pattern recognition receptors in innate immunity: update on toll-like receptors. Nat. Immunol., 11: 327-384.
Kim, IH; Na, KJ and Yang, MP (2005). Immune responses during the peripartum period in dairy cows with postpartum endometritis. J. Reprod. Develop. 6: 757-764.
Koneman, EW; Minckler, TM; Shires, DB and De Jonch, DS (1971). Postmortem bacteriology: II. Selection of cases for culture. Am. J. Clin. Pathol., 55: 17-23.
Lange, J; McCarthy, A; Kay, J; Meier, S; Walker, C; Crookenden, MA; Mitchell, MD, Loor, JJ; Roche, JR and Heiser, A (2016). Prepartum feeding level and body condition score affect immunological performance in grazing dairy cows during the transition period. J. Dairy Sci., 99: 2329-2338.
Mahnani, A; Sadeghi-Sefidmazgi, A and Cabrera, VE (2015). Consequences and economics of metritis in Iranian Holstein dairy farms. J. Dairy Sci., 98: 6048-6057.
Miyake, K (2006). Invited review: roles for accessory molecules in microbial recognition by toll-like receptors. J. Endotoxin Res., 12: 195-204.
O’Neill, LA; Bryant, CE and Doyle, SL (2009). Therapeutic targeting of toll-like receptors for infectious and inflammatory diseases and cancer. Pharmacol. Rev., 61: 1073-1094.
Reppert, EJ (2015). Evidence for the use of ceftiofur for treatment of metritis in dairy cattle. Vet. Clin. Anim. Pract., 31: 139-149.
Schöniger, S; Gräfe, H and Schoon, HA (2017). Expression of toll-like receptors 2, 4 and 6 in different cell populations of the equine endometrium. Vet. Immunol. Immunopathol., 185: 7-13.
Sheldon, IM and Dobson, H (2004). Postpartum uterine health in cattle. Anim. Reprod. Sci., 82: 295-306.
Sheldon, IM; Lewis, GS; LeBlanc, S and Gilbert, RO (2006). Defining postpartum uterine disease in cattle. Theriogenology. 65: 1516-1530.
Sheldon, IM; Noakes, D; Rycroft, A; Pfeiffer, D and Dobson, H (2002). Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction. 123: 837-845.
Sheldon, IM; Rycroft, AN; Dogan, B; Craven, M; Bromfield, JJ; Chandler, A; Roberts, MH; Price, SB; Gilbert, RO and Simpson, KW (2010). Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. Plos One. 5: e9192.
Swangchan-Uthai, T; Lavender, CR; Cheng, Z; Fouladi-Nashta, AA and Wathes, DC (2012). Time course of defense mechanisms in bovine endometrium in response to lipopolysaccharide. Biol. Reprod., 87: 131-113, 135.
Takeuchi, O and Akira, S (2010). Pattern recognition receptors and inflammation. Cell. 140: 805-820.
Takeuchi, O; Hoshino, K and Akira, S (2000). Cutting edge: TLR2-deficient and MyD88-deficient mice are highly susceptible to Staphylococcus aureus infection. J. Immunol., 165: 5392-5396.
Triantafilou, M and Triantafilou, K (2002). Lipopoly-saccharide recognition: CD14, TLRs and the LPS-activation cluster. Trends Immunol., 23: 301-304.
van der Molen, RG; Schutten, JHF; van Cranenbroek, B; ter Meer, M; Donckers, J; Scholten, RR; van der Heijden, OWH; Spaanderman, MEA and Joosten, I (2014). Menstrual blood closely resembles the uterine immune micro-environment and is clearly distinct from peripheral blood. Hum. Reprod., 29: 303-314.
Williams, EJ; Fischer, DP; Pfeiffer, DU; England, GC; Noakes, DE; Dobson, H and Sheldon, IM (2005). Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology. 63: 102-117.
Yapan, S; Liping, S; Aizhen, G and Liguo, Y (2014). Effects of toll-like receptor 2 gene mutation on resistance to bovine brucellosis. Livest. Sci., 170: 30-34.
Yoshimura, A; Lien, E; Ingalls, R; Heine, H; Henneke, P; Teti, G; Espevik, T; Kato, I and Golenbock, D (2000). Recognition of gram-positive bacterial cell wall components by the innate immune system occurs via toll-like receptor 2. J. Endotoxin Res., 6: S13-S13.
Zähringer, U; Lindner, B; Inamura, S; Heine, H and Alexander, C (2008). TLR2-promiscuous or specific? A critical re-evaluation of a receptor expressing apparent broad specificity. Immunobiology. 213: 205-224.