Impact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos

Document Type : Full paper (Original article)

Authors

1 Veterinary Department, Egyptian Armed Forces, Cairo, Egypt, and Ph.D. Student, Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt

2 Department of Animal Reproduction and A.I., National Research Center, Dokki, Giza, Egypt

3 Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt

Abstract

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes were matured and fertilized in vitro by fresh and frozen semen. After embryos evaluation, good quality morula and blastocysts were vitrified using 0.25 ml straws and the post-warmed viability was assessed by further culture for 24 h. There was no significant difference in cleavage rate between embryos derived from fresh and frozen semen, whereas the rate of embryo development to the morula (P<0.05) and blastocysts (P<0.01) stages was significantly decreased in embryos derived from frozen compared to fresh semen. After warming the vitrified embryos, there was no significant difference between embryos derived from fresh and frozen semen in the percentages of morphologically viable embryos. However, 24 h after culture, the rate of morphologically normal and survived embryos was increased (P<0.05) in embryos derived from fresh compared to the frozen semen. In conclusion, in buffalo, the use of fresh semen could improve the rate of embryo development and their crytolerance compared to the frozen semen.

Keywords


Abdel Dayem, AMH; Mahmoud, KGhM; Nawito, MF; Ayoub, MM and Scholkamy, TH (2009). Fertility evaluation in Egyptian buffalo bulls using zonapellucida binding and in vitro fertilization assays. Livest. Sci., 122: 193-198.
Abd El-Fatah, EM; El-Behairy, AM; Amin, AS and Eltablawy, NA (2008). Effect of cryopreservation on DNA integrity, lipid peroxidation and antioxidation enzyme activity in buffalo spermatozoa. Egypt. J. Basic Appl. Physiol., 7: 467-481.
Brackett, BG and Oliphant, G (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod., 12: 260-274.
Edgar, DH; Bourne, H; Speirs, AL and McBain, JC (2000). A quantitative analysis of the impact of cryopreservation on the implantation potential of human early cleavage stage embryos. Hum. Reprod., 15: 175-179.
El-Naby, AlAlHH; Mahmoud, KGhM; Scholkamy, TH; Sosa, GAM; Ahmed, YF and Abouel-Roos, MEA (2016). Influence of epidermal growth factor with cysteamine on in-vitro buffalo embryo development. Egypt. J. Vet. Sci., 47: 27-39.
El-Naby, AlAlHH; Mahmoud, KGhM; Sosa, GAM; Abouel-Roos, MEA and Ahmed, YF (2017). Effect of using ascorbic acid and cysteamine supplementation on in-vitro development of buffalo embryos. Asian Pacific J. Reprod., 6: 85-88.
El-Sisy, GA; ElNatat, WS and El-Sheshtawy, RI (2007). Buffalo semen quality, antioxidants and peroxidation during chilling and cryopreservation. Online J. Vet. Res., 11: 55-61.
Englert, Y; Delvigne, A; Vekemans, M; Lejeune, B; Henlisz, A; De Maertelaer, G and Leroy, F (1989). Should one use fresh or frozen sperm in in-vitro fertilization with donors? Acta Urol Belg., 57: 83-91.
Gasparrini, B (2002). In vitro embryo production in buffalo species: state of the art. Theriogenology. 57: 237-256.
Gordon, I (1996). Controlled reproduction in farm animals series: controlled reproduction in cattle and buffaloes. CAB International, UK.
Janny, L and Ménézo, YJ (1994). Evidence for a strong paternal effect on human preimplantation embryo development and blastocyst formation. Mol. Reprod. Dev., 38: 36-42.
Januskauskas, A and Žilinskas, H (2002). Bull semen evaluation post-thaw and relation of semen characteristics to bull’s fertility. Vet. Zootec., 17: 29-36.
Lehloenya, KC; Mahoete, N; Greyling, JPC and Nedambale, TL (2010). Effect of breed and frozen-thawed ram semen on in vitro fertilization and ovine embryonic development. Reprod. Fertil. Dev., 23: 170.
Mahmoud, KGhM (2010). Vitrification of oocytes and embryos for conservation of animal genetic resources. Twenty-Two Annual Congress Egyptian Society. Animal Reproduction Fert. Cairo and RasSedr, 13-17 February. PP: 75-114.
Mahmoud, KGhM; El-Sokary, AAE; Abdel-Ghaffar, AE; Abou El-Roos, MEA and Ahmed, YF (2015a). Analysis of chromatin integrity and DNA damage in cryopreserved buffalo semen. Iran. J. Vet. Res., 16: 161-166.
Mahmoud, KGhM; El-Sokary, AAE; Abou El-Roos, MEA; Abdel-Ghaffar, AE and Nawito, M (2013). Sperm characteristics in cryopreserved buffalo bull semen in relation to field fertility. Iran. J. Appl. Anim. Sci., 3: 777-783.
Mahmoud, KGhM; El-Sokary, MMM; Kandiel, MMM; Abouel-Roos, MEA and Sosa, GMS (2016). Effect of
cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes. Iran. J. Vet. Res., 17: 165-170.
Mahmoud, KGhM and Nawito, MF (2005). Influence of serum source on buffalo in vitro embryonic development with reference to heat inactivation. Livest. Prod. Sci., 96: 285-289.
Mahmoud, KGhM; Scholkamy, TH and Darwish, SF (2015b). Improvement of vitrification of in vitro produced buffalo embryos with a special reference to sex ratio following vitrification. Iran. J. Vet. Res., 16: 325-330.
Mahmoud, KGhM and Seidel, JrGE (2010). Viability and DNA fragmentation assessment of bovine embryos vitrified by different methods. Researcher. 2: 14-20.
Mahmoud, KGhM; Ziada Maha, S and Scholkamy, TH (2004). Freezability of buffalo semen in relation to its in-vitro fertilizing capacity and chromosomal picture. J. Egypt. Vet. Med. Assoc., 64: 239-251.
Mukhopadhyay, CS; Gupta, AK; Yadav, BR; Chauhan, IS; Gupta, A; Mohanty, TK and Raina, VS (2011). Effect of cryopreservation on sperm chromatin integrity and fertilizing potential in bovine semen. Livest. Sci., 136: 114-121.
Niwa, K and Ohgoda, O (1988). Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology. 30: 733-741.
Pugh, PA; Fukui, Y; Tervit, HR and Thompson, JG (1991). Developmental ability of in vitro matured sheep oocytes collected during the nonbreeding season and fertilized in vitro with frozen ram semen. Theriogenology. 36: 771-778.
Romao, R; Marques, CC; Baptista, MC; Vasques, MI; Barbas, JP; Horta, AEM; Carolino, N; Bettencourt, E; Plancha, C; Rodrigues, P and Pereira, RM (2013). Evaluation of two methods of in vitro production of ovine embryos using fresh or cryopreserved semen. Small Rum. Res., 110: 36-41.
Saacke, RG; Dalton, JC; Nadir, S; Nebel, RL and Bame, JH (2000). Relationship of seminal traits and insemination time to fertilization rate and embryo quality. Anim. Reprod. Sci., 60: 663-677.
Sanchez, R; Stale, T; Khanago, O; Turley, H; Gips, H and Schill, WB (1996). Sperm selection methods for intracytoplasmic sperm injection (ICSI) and andrological patients. J. Assist. Reprod. Gen., 13: 228-233.
Scholkamy, TH; Mahmoud, KGhM; El Zohery, FA and Ziada, MS (2009). Evaluation of sephadexfiltration for freezability and in vitro fertilizing ability of buffalo semen. Glob. Vet., 3: 144-150.
Shoukir, Y; Chardonnens, D; Campana, A and Sakkas, D (1998). Blastocyst development from supernumery embryos after in-tracytoplasmic sperm injection: a paternal influence? Hum. Reprod., 13: 1632-1637.
Watson, PF (1995). Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev., 7: 871-891.
Zhang, BR; Larson, B; Lundeheim, N and Roderiguez Martinez, H (1998). Sperm characteristics and zonapellucida binding in relation to field fertility of frozen-thawed semen from dairy AI bulls. Int. J. Androl., 21: 207-216.