آلودگی با انتروتوکسیژنیک Escherichia coli موجب بیان پروتئین‌های اتصالات محکم در موش می‌شود

نوع مقاله : مقاله کامل

نویسندگان

چکیده

انتروتوکسیژنیک Escherichia coli (ETEC)موجب بروز اسهال در مسافران، کودکان و طوله خوک‌ها می‌شود ولی مکانیسم اصلی که ETEC از طریق آن سبب بروز اسهال می‌شود به طور کامل شناخته نشده است. تحقیقات اخیر نشان داده‌اند که پروتئین‌های اتصالات محکم (TJ) و همچنین آکواپورین 3 (AQP 3) از فاکتور‌های دخیل در اسهال باکتریایی هستند. در این مطالعه با استفاده از روش‌های ایمونوبلاتینگ و ایمونوهیستوشیمی، مشاهده کردیم که ETEC سبب افزایش فراوانی پروتئین‌های TJ occludin)، claudin-1، (zonula occludens-1 در موش می‌شود. ETEC از طریق مسیر‌های کیناز زنجیره سبک میوزین (MLCK) و زنجیره سبک تنظیمی میوزین ІІ (MLC20) موجب افزایش بیان پروتئین‌های TJ می‌شود و در عین حال اثر کمی بر فعال کردن مسیر phosphatidylinositol 3-kinase (PI3K)-Akt دارد. عفونت با ETEC اثر کمی بر فراوانی پروتئین آکواپورین 3 دارد. در مجموع عفونت با ETEC فراوانی پروتئین TJ روده‌ای را تحت تاثیر قرار می‌دهد که این مساله بیان کننده اهمیت پروتئین‌های TJ در اسهال ناشی از ETEC است.

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