Histological and biochemical evaluation of supplementing broiler diet with β-hydroxy-methyl butyrate calcium (β-HMB-Ca)

Document Type : Full paper (Original article)

Authors

1 Department of Animal Production, College of Agriculture, University of Baghdad, Baghdad, Iraq

2 Department of Anatomy, Histology and Embryology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq

Abstract

Two hundred and sixteen day-old Ross-308 broiler chicks were allocated into 4 groups to study the impacts of different concentrations (0.0, 0.1, 0.15 and 0.2%) of β-hydroxy-methyl butyrate calcium (β-HMB-Ca), on values of tri-iodothyronin (T3) and tetra-iodothyronin (T4) hormones, liver enzymes [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)], uric acid, peroxide, malondialdihyde (MDA), fatty acids and some histological parameters of small intestine (thickness of mucosa, height of villi, thickness of villi, depth of epithelial crypts and epithelial height). The biochemical results did not show any significant effect on T3 and T4 hormones and ALT while there was significant (P<0.01) decrease of AST in groups 2 and 3 and significant (P<0.05) decrease in uric acid in groups 2, 3 and 4 in comparison to control. In the liver, peroxide value (PV) and free fatty acids (FFA) were significantly (P<0.05 and P<0.01 respectively) decreased in groups 2 and 3 compared to control. The histological changes indicate significant values (P<0.05) in all parameters of duodenum in group 2 and 3, while those parameters of jejunum showed significant values (P<0.05) in most parameters of groups 2 and 4. In conclusion, the addition of β-HMB-Ca to the broiler diet from age 1 to 35 days has improved the levels of liver function enzymes and uric acid in the serum and lowered the parameters of oxidation in the liver with improved the maturity, performance and secretory activities of the small intestine in broiler chickens.

Keywords

References

Bancroft, JD and Marilyn, G (2008). Theory and practice of histological techniques. 1st Edn., London, Elsevier Limited. PP: 168-173.
Blachier, F; Mariotti, F; Huneau, JF and Tome, D (2007). Effects of amino acid-derived luminal metabolites on the colonic epithelium and physiopathological consequences. Amino Acids. 33: 547-562.
Brioche, T; Pagano, AF; Py, G and Chopard, A (2016). Muscle wasting and aging: experimental models, fatty infiltrations, and prevention. Mol. Aspects Med., 50: 56-87.
Britton, KE; Quinn, EV; Brown, BL and Eknis, PP (1957). A strategy for thyroid function tests. Br. Med. J., 3: 350-360.
Buyse, J; Swennen, Q; Vandemaele, F; Klasing, KC; Niewold, TA; Baumgartner, M and Goddeeris, BM (2009). Dietary β-hydroxy-β-methyl butyrate supplemen-tation influences performance differently after immunization in broiler chickens. J. Anim. Physiol. Anim. Nutr., 93: 512-524.
Davey, MW; Stals, E; Panis, B; Keulemans, J and Swennen, RL (2005). High-throughput determination of malondial-dihyde in plant tissues. Ana. Biochem., 347: 201-207.
Egan, H; Kirk, RS and Sawyer, R (1981). Pearsons chemical analysis of foods. 8th Edn., Edinburgh, UK, Churchill Livingstone. PP: 325-329.
Eley, HL; Russell, ST; Baxter, JH; Mukerji, P and Tisdale, MJ (2007). Signaling pathways initiated by beta-hydroxy-beta-methyl butyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli. Am. J. Physiol. Endocrinol. Metab., 293: 923-931.
EL-Kafoury, MA; Kalleny, NK and Hamam, GG (2011). Effect of amino acid L-leucine on the musculo-skeletal changes during cast-immobilization I adult male Albino rats. Physiological and histological study. Life Sci., 8: 876-892.
Ferguson, LD and Walters, MR (2011). Xanthine oxidase inhibition for the treatment of stroke disease: a novel therapeutic approach. Exp. Rev. Cardiovasc. Ther., 9: 399-401.
Foya, OT and Ferket, PR (2006). Effect of in vivo feeding egg with protein, beta-hydroxy-beta-methyl butyrate and carbohydrate on glycogen status and neonatal growth of turkeys. Poul. Sci., 85: 1185-1192.
Fuller, JC; Arp, LH; Diehl, LM; Landin, KL; Baier, SM and Rathmacher, JA (2014). Subchronic toxicity study of b-hydroxy-b-methyl butyric free acid in Sprague Dawley rats. Food Chem. Toxicol., 67: 145-153.
Fuller, JC; Sharp, RL; Angus, HF; Khoo, PY and Rathmacher, JA (2015). Comparison of availability and plasma clearance rates of β-hydroxy-β-methyl butyrate delivery in the free acid and calcium salt forms. Br. J. Nutri., 114: 1403-1409.
Gallagher, PM; Carrithers, JA; Goodard, MP; Schulze, KE and Trappe, SW (2000). β-Hydroxy-β-methyl butyrate ingestion, Part II: Effects on hematology, hepatic and renal function. Med. Sci. Sports Exerc., 32: 2116-2119.
Gerlinger-Romero, F; Guimarães-Ferreira, L; Giannocco, G and Nunes, MT (2011). Chronic supplementation of beta-hydroxy-beta methyl butyrate (HMB) increases the activity of the GH/IGF-I axis and induces hyperinsulinemia in rats. Growth Horm. IGF Res., 21: 57-62.
Helen, J; Mukerji, P and Michael, J (2005). Tisdale attenuation of proteasome-induced proteolysis in skeletal muscle by beta-hydroxy-beta-methyl butyrate in cancer-induced muscle. Loss. Cancer Res., 65: 277-283.
Holecek, M; Muthny, L; Kovarik, M and Sispera, L (2009). Effect of beta-hydroxy-beta-methyl butyrate (HMB) on protein metabolism in whole body and in selected tissues. Food and Chem. Toxicol., 47: 255-259.
Janero, DR (1990). Malondialdihyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic. Biol. Med., 9: 515-540.
Khudair, IM and Al-Hussary, NA (2010). Effect of vaccination on some biochemical parameters in broiler chickens. Iraqi J. Vet. Sci., 24: 59-64.
Kohlmeier, M (2015). Leucine nutrient metabolism: structures, functions, and genes. 2nd Edn., USA, Academic Press. PP: 385-388.
Kondo, H; Yumoto, K; Alwood, JS; Mojarrab, R; Wang, A; Almeida, EA; Searby, ND; Limoli, CL and Globus, RK (2010). Oxidative stress and gamma radiation-induced cancellous bone loss with musculoskeletal disuse. J. Appl. Physiol., 108: 152-161.
Kornasio, R; Riederer, I; Butler-Browne, G; Mouly, V; Uni, Z and Halevy, O (2009). β-hydroxy-β-methyl butyrate (HMB) stimulates myogenic cell proliferation, differentiation and survival via the MAPK/ERK and PI3K/Akt pathways. Biochim. Biophys. Acta BBA-Mol. Cell Res., 1793: 755-763.
Mahfouz, MK; Zyan, KAM and Abdel-Hamid, HH (2016). Biochemical effect of β-hydroxy-β-methyl butyrate calcium and creatine supplementations on some blood parameters, pro-inflammatory cytokines and growth performance of broiler chicks. Benha Vet. Med. J., 30: 39-50.
Mandal, AK and Mount, DB (2015). The molecular physiology of uric acid homeostasis. Annu. Rev. Physiol., 77: 323-345.
Mauch, DH; Nagler, K; Schumacher, S; Goritz, C; Muller, EC; Otto, A and Pfrieger, FW (2001). CNS synapto-genesis promoted by glia-derived cholesterol. Science. 294: 1354-1357.
Moraes, R; Bavel, DV; Moraes, BS and Tibiriçá, E (2014). Effects of dietary creatine supplementation on systemic microvascular density and reactivity in healthy young adults. Nutrition J., 13: 115-124.
Mullur, R; Liu, YY and Brent, GA (2014). Thyroid hormone regulation of metabolism. Physiol. Rev., 94: 355-382.
Nissen, S and Abumrad, NN (1997). Role of the leucine metabolite β-hydroxy β-methyl butyrate (HMB). J. Nutrt. Biochem., 8: 300-311.
Nissen, S; Fuller, JC; Sell, J; Ferket, PR and Rives, DV (1994). The effect of β-hydroxy-β-methylbutyrate on growth, mortality, and carcass qualities of broiler chickens. Poult. Sci., 73: 137-155.
Nissen, S; Sharp, L and Panton, M (2000). B-hydroxy-beta-methyl butyrate (HMB) supplementation humans is safe and may decrease cardiovascular risk factors. Am. Soc. Nutri. Sci., 30: 1937-1945.
NRC (1994). National research council nutrent requirement for poultry. 9th Edn., USA, National Academy Press. PP: 19-26.
Ostaszewski, P; Kostiuk, S; Balasińska, B; Jank, M; Papet, I and Glomot, F (2000). The leucine metabolite 3-hydroxy-3-methylbutyrate (HMB) modifies protein turnover in muscles of laboratory rats and domestic chickens in vitro. J. Anim. Physiol. Anim. Nutr., 84: 1-8.
Pacher, P; Nivorozhkin, A and Szabo, C (2006). Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol. Rev., 58: 87-114.
Portal, S; Zadik, Z; Rabinowitz, J; Pilz-Burstein, R; Adler-Portal, D and Meckel, Y (2011). The effect of HMB supplementation on body composition, fitness, hormonal and inflammatory mediators in elite adolescent volleyball players: a prospective randomized, double-blind, placebo-controlled study. Eur. J. Appl. Physiol., 111: 2261-2269.
Qiao, X; Zhang, HJ; Wu, SG; Yue, HY; Zuo, JJ; Feng, DY and Qi, GH (2013). Effect of β-hydroxy-β-methyl butyrate calcium on growth, blood parameters, and carcass qualities of broiler chickens. Poul. Sci., 92: 753-759.
Retiman, S and Frankel, S (1957). A colorimeteric method for determination of serum glutamic oxaloacetic pyruvic transaminase. Am. J. Clin. Path., 28: 56-63.
Robertson, SP; Twigg, SR; Sutherland-Smith, AJ; Biancalana, V; Gorlin, RJ; Horn, D; Kenwrick, SJ; Kim, CA; Morava, E; Newbury-Ecob, R; Orstavik, KH; Quarrell, OW; Schwartz, CE; Shears, DJ; Suri, M; Kendrick-Jones, J and Wilkie, AO (2003). Localized mutations in the gene encoding the cytoskeletal protein filamin A cause diverse malformations in humans. Nat. Genet., 33: 487-491.
Rodwell, VW (2009). Metabolism of purine and pyrimidine nucleotides. Harper’s illustrated biochemistry.-28e. New York, McGraw Hill. PP: 30-292.
Routhier, DD and Stacy, JJ (2007). HMB use and its relationship to exercise-induced muscle damage and performance during exercise. Int. Sport Med. J., 2: 68-77.
Schindelin, J; Rueden, CT and Hiner, MC (2015). The Image J ecosystem: an open platform for biomedical image analysis, Molecular Reproduction and Development, PMID 26153368 (on Google Scholar).
Siu, PM; Pistilli, EE and Always, SE (2008). Age-dependent increase in oxidative stress in gastrocnemius muscle with unloading. J. Appl. Physiol., 105: 1695-1705.
Szcześniak, KA; Ostaszewski, P; Fuller, JC; Ciecierska, A and Sadkowski, T (2015). Dietary supplementation of β-hydroxy-β-methylbutyrate in animals. J. Anim. Physiol. Anim. Nutr., 99: 405-417.
Tako, E; Ferkt, PR and Uni, Z (2004). Effect of in vivo feeding on carbohydrate and beta-hydroxy-beta-methyl butyrate on the development of chick intestine. Poult. Sci., 83: 2023-2028.
Teitz, NW (1987). Fundamental of clinical chemistry. 2nd Edn., Philadelphia, PA, Saunders Co., PP: 47-53.
Van-Koevering, MT; Dolezal, HG; Gill, DR; Owens, FN; Strasia, CA; Buchanan, DS; Lake, R and Nissen, S (1994). Effects of beta-hydroxy-beta-methyl butyrate on performance and carcass quality of feedlot steers. J. Anim. Sci., 72: 1927-1935.
Wan, H; Zhu, J; Su, G; Liu, Y; HUa, L; HU, L; Wu, C; Zhang, R; Zhou, P; Shen, Y; Lin, Y; Xu, S; Fang, Z; Che, L; Feng, B and Wu, D (2016). Dietary supplementation with β-hydroxy-β-methyl butyrate calcium during the early postnatal period accelerates skeletal muscle fiber growth and maturity in intra-uterine growth-retarded and normal-birth-weight piglets. Br. J. Nutri., 115: 1360-1369.
Wang, CS; Chang, TT; Yao, WJ; Wang, ST and Chou, P
(2012). Impact of increasing alanine aminotransferase levels within normal range on incident diabetes. J. Formosan Med. Asso., 111: 201-208.
Wilkinson, DJ; Hossain, T; Hill, DS; Phillips, BE; Crossland, H; Williams, J; Loughna, P; Churchward-Venne, TA; Breen, L; Phillips, SM; Etheridge, T; Rathmacher, JA; Smith, K; Szewczyk, NJ and Atherton, PJ (2013). Effects of leucine and its metabolite β-hydroxy-β-methyl butyrate on human skeletal muscle protein metabolism. J. Physiol., 591: 2911-2923.
Witta, VC; Krause, GF and Bailey, ME (1970). A new extraction method for determination thiobarbituric acid values of pork and beef during storage. J. Food Sci., 35: 582-585.
Wu, H; Xia, Y; Jiang, J; Du, H; Guo, X; Liu, X; Li, C; Huang, G and Niu, K (2015). Effect of beta-hydroxy-beta-methyl butyrate supplementation on muscle loss in older adults: a systematic review and meta-analysis. Arch. Gerontol. Geriatrics. 61: 168-175.
 
 

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