Effect of time of eCG administration on the fate of ovarian follicle in Holstein heifers

Document Type : Full paper (Original article)


1 Resident of Theriogenology, Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


The objective of this study was to investigate the effect of equine chorionic gonadotropin (eCG) on ovarian follicles at three stages of development (emergence, dominance and early static phases) during the first follicular wave (FFW) in Holstein heifers. Heifers (n=20) were randomly assigned into four experimental groups (n=5 in each group). Heifers received eCG (500 IU; Folligon®; Intervet, Holland; i.m) a) on the day of follicle emergence (day of ovulation; group 1), b) on the dominant phase (dominant follicle (DF): the first day in which follicle was observed at ≥10 mm; group 2, and c) on the early static phase (group 3) of the FFW. Control group heifers did not receive any treatment. Daily ultrasonography was conducted to monitor ovarian structure throughout estrous cycle. All treatment group heifers, regardless of the stage of follicle development, displayed follicle growth after eCG injection. Administration of eCG, in group 1, hastened DF detection and induced co-dominant follicles; whereas, in groups 2 and 3, it delayed DF regression, and increased cycle length compared to control. In all treatment group heifers, DF was present 84 h after eCG injection. Maximum diameter of corpus luteum was larger in eCG treated groups compared to control (P<0.05). In conclusion, depending on the time of eCG administration throughout the FFW (emergence, dominant and early static phases), co-dominancy, maintenance of DF, enhancement of follicle and corpus luteum growth and increase in estrous cycle length could be observed in Holstein heifers.


Adams, GP (1999). Comparative patterns of follicular development and selection in ruminants. J. Reprod. Fertil., 54: 17-32.
Adams, GP; Matteri, RL; Kastelic, JP; Ko, JC and Ginther, OJ (1992). Association between surges of follicle-stimulating hormone and the emergence of follicular waves in heifers. J. Reprod. Fertil., 94: 177-188.
Ahmad, N; Townsend, EC; Dailey, RA and Inskeep, EK (1997). Relationship of hormonal patterns and fertility to ocurrence of two or three waves of ovarian follicles, before and after breeding, in beef cows and heifers. Anim. Reprod. Sci., 49: 13-28.
Bartolome, JA; Perez Wallace, S; de la Sota, RL and Thatcher, WW (2012). The effect of administering equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) post artificial insemination on fertility of lactating dairy cows. Theriogenology. 78: 1110-1116.
Bevers, MM; Dieleman, SJ; van Tol, HTM; Blankenstein, DM and van den Broek, J (1989). Changes in pulsatile secretion patterns of LH, FSH, progesterone, androstenedione and oestradiol in cows after superovulation with PMSG. J. Reprod. Fertil.,87: 745-754.
Bo, GA; Adams, GP; Caccia, M; Martinez, M; Pierson, RA and Mapletoft, RJ (1995). Ovarian follicular wave emergence after treatment with progesterone and estradiol in cattle. Anim. Reprod. Sci., 39: 193-204.
Burke, CR; Day, ML; Bunt, CR and Macmillan, KL (2000). Use of a small dose of estradiol benzoate during diestrus to synchronize development of the ovulatory follicle in cattle. J. Anim. Sci., 78: 145-151.
Dieleman, SJ; Bevers, MM; Wurth, YA; Gielen, JT and Willemse, AH (1989). Improved embryo yield and condition of donor ovaries in cows after PMSG superovulation with monoclonal anti-PMSG administered shortly after the preovulatory LH peak. Theriogenology. 31: 473-487.
Fátima, LA; Baruselli, PS; Gimenes, LU; Binelli, M; Rennó, FP; Murphy, BD and Papa, PC (2012). Global gene expression in the bovine corpusluteum is altered after stimulatory and superovulatory treatments. Reprod. Fertil. Dev., 25: 998-1011.
Garcia-Ispierto, I; López-Helguera, I; Martino, A and López-Gatius, F (2012). Reproductive performance of anoestrous high-producing dairy cows improved by adding equine chorionic gonadotrophin to a progesterone-based oestrous synchronizing protocol. Reprod. Dom. Anim., 47: 752-758.
Ginther, OJ (1970). Effect of progesterone on length of oestrous cycle in cattle. Am. J. Vet. Res., 31: 493-496.
Ginther, OJ; Bergfelt, DR; Kulick, LJ and Kot, K (2000). Selection of the dominant follicle in cattle: role of estradiol. Biol. Reprod., 63: 383-389.
Ginther, OJ; Kastelic, JP and Knopf, L (1989b). Composition and characteristics of follicular waves during the bovine estrous cycle. Anim. Reprod. Sci., 20: 187-200.
Ginther, OJ; Knopf, L and Kastelic, JP (1989a). Temporal associations among ovarian events in cattle during oestrous cycle with two and three follicular waves. J. Reprod. Fertil., 41: 154-247.
Gonzalez-Menico, F; Manns, J and Murphy, BD (1978). FSH and LH activity of PMSG from mares at different stages of gestation. Anim. Reprod. Sci., 1: 137-144.
Gonzalez-Padilla, E; Niswender, GD and Wiltbank, JN (1975). Puberty in beef heifers. II. Effect of injections of progesterone and estradiol-17β on serum LH, FSH and ovarian activity. J. Anim. Sci., 40: 1105-1109.
Goulding, D; Williams, DH; Rochei, JF and Boland, MP (1996). Factors affecting superovulations in heifers treated with PMSG. Theriogenology. 45: 765-773.
Inskeep, EK; Braden, TD; Lewis, PE; Garcia-Winder, M and Niswender, GD (1988). Receptors for luteinizing hormone and follicle-stimulating hormone in largest follicles of postpartum beef cows. Biol. Reprod., 38: 587-591.
Knopf, L; Kastelic, JP; Scallenberger, E and Ginther, OJ (1989). Ovarian follicular dynamics in heifers: test of two-wave hypothesis by ultrasonography monitoring individual follicles. Dom. Anim. Endocrinol., 6: 111-119.
Macmillan, KL and Thatcher, WW (1991). Effect of an agonist of gonadotropin-releasing hormone on ovarian follicle in cattle. Biol. Reprod., 45: 883-889.
Moghaddam, AA; Niasari-Naslaji, A and Bolorchi, M (2001). Effect of steroid and GnRH, given on the day of estrus, on ovarian follicle characteristics in Holstein heifers. J. Fac. Vet. Med., 56: 45-52.
Monniaux, D; Chupin, D and Saumande, J (1983). Superovulatory responses of cattle. Theriogenology. 19: 55-81.
Murphy, BD (2012). Equine chorionic gonadotropin: an enigmatic but essential tool. Anim. Reprod., 9: 223-230.
Murphy, BD and Martinuk, SD (1991). Equine chorionic gonadotropin. Endocrinol. Rev., 12: 27-44.
Newcomb, R; Christie, WB; Rowson, LEA; Walters, DE and Bousfield, WED (1979). Influence of dose, repeated treatment and batch of hormone on ovarian response in heifers treated with PMSG. J. Reprod. Fertil., 56: 113-118.
Niasari-Naslaji, A; Eslami, M and Nazem, Y (2012). Ovulatory response of different GnRH analogues and subsequent corpus luteum lifespan in the presence of norgestomet in Holstein heifers. Iran. J. Vet. Res., 13: 36-41.
Niasari-Naslaji, A; Hosseini, SM; Sarhaddi, F; Bolourchi, M and Birjandi, MR (2001). Steriod priming shortnes prostaglandin-based estrus synchronization program from 14 to 7 days in cattle. Theriogenology. 56: 735-743.
Núnez-Olivera, R; de Castroa, T; García-Pintos, C; Bó, G; Piaggio, J and Menchaca, A (2014). Ovulatory response and luteal function after eCG administration at the end of a progesterone and estradiol based treatment in postpartum anestrous beef cattle. Anim. Reprod. Sci., 146: 111-116.
Odde, KG; Wards, HS; Kiracofe, GH; McKee, RJ and Kittok, RJ (1980). Short estrous cycles and associated serum progesterone levels in beef cows. Theriogenology. 14: 105-112.
O’Hara, L; Forde, N; Duffy, P; Randi, F; Kelly, AK; Vaenza, A; Rodriguez, P and Lonergan, P (2016). Effect of combined exogenous progesterone with luteotrophic support via equine chorionic gonadotrophin (eCG) on corpus luteum development, circulating progesterone concentration and embryo development in cattle. Reprod, Fertil. Dev., 28: 269-277.
Pessoa, GA; Martini, AP; Carloto, GW; Rodrigues, MCC; Claro Júnior, I; Baruselli, PS; Brauner, CC; Rubin, MIB; Corrêa, MN; Leivas, FG and Sá Filho, MF (2016). Different doses of equine chorionic gonadotropin on ovarian follicular growth and pregnancy rate of suckled Bos taurus beef cows subjected to timed artificial insemination protocol. Theriogenology. 85: 792-799.
Pulley, SL; Wallace, LD; Mellieon, HIJr and Stevenson, JS (2013). Ovarian characteristics, serum concentrations of progesterone and estradiol, and fertility in lactating dairy cows in response to equine chorionic gonadotropin. Theriogenology. 79: 127-134.
Rigoglio, NN; Fátima, LA; Hanassaka, JY; Pinto, GL; Machado, ASD; Gimenes, LU; Baruselli, PS; Rennó, FP; Moura, CEB; Watanabe, IS and Papa, PC (2013). Equine chorionic gonadotropin alters luteal cell mor-phologic features related to progesterone synthesis. Theriogenology. 79: 673-679.
Rostami, B; Niasari-Naslaji, A; Vojgani, M; Nikjou, D; Amanlou, HM and Gerami, A (2011). Effect of eCG on early resumption of ovarian activity in postpartum dairy cows. Anim. Reprod. Sci., 128: 100-106.
Sá Filho, MF; Ayres, H; Ferreira, RM; Marques, MO; Reis, EL; Silva, RC; Rodrigues, CA; Madureira, EH; Bó, GA and Baruselli, PS (2010). Equine chorionic gonadotropin and gonadotropin-releasing hormone enhance fertility in a norgestomet-based, timed artificial insemination protocol in suckled Nelore (Bos indicus) cows. Theriogenology. 73: 651-658.
Savio, JD; Keenan, L; Boland, MP and Roche, JF (1988). Pattern of growth of dominant follicles during the oestrous cycle of heifers. J. Reprod. Fertil., 83: 663-671.
Savio, JD; Thatcher, WW; Badinga, L; de la Sota, RL and Wolfeson, D (1993). Regulation of dominant follicle turnover during the estrous cycle in cows. J. Reprod. Fertil., 97: 197-203.
Schams, D; Mentzer, C; Schallenberger, E; Hahn, J and Hahn, R (1978). Some studies of pregnant mare serum gonadotropin and on endocrine responses after application for superovulation in cattle. In: Sreenan J (Ed.), Control of reproduction in the cow. (8th Edn.), The Hague: Martinus Nijhoff. PP: 122-143.
Sirois, J and Fortune, JE (1998). Ovarian follicular dynamics during the estrous cycle in heifers monitored by real-time ultrasonography. Biol. Reprod., 39: 308-317.
Sirois, J and Fortune, JE (1990). Lengthening the bovine estrous cycle with low levels of exogenous progesterone: a model for studying ovarian follicular dominance. Endocrinology. 127: 916-925.
Soumano, K; Lussier, JG and Price, CA (1998). Levels of messenger RNA encoding ovarian receptors for FSH and LH in cattle during superovulation with equine chorionic gonadotropin versus FSH. J. Endocrinol., 156: 373-378.
Souza, AH; Cunha, AP; Silva, EPB; Gümen, A; Ayres, H; Guenther, JN and Wiltbank, MC (2009). Comparison of gonadorelin products in lactating dairy cows: efficacy based on induction of ovulation of an accessory follicle and
circulating luteinizing hormone profiles. Theriogenology. 72: 271-279.
Souza, AH; Wosniacki, AM; Torres-Junior, JRS; Martins, CM; Ayres, H and Baruselli, PS (2006). Factors that affect the corpus luteum volume during estrous cycle in high producer Holstein cows. Acta Sci. Vet., 34: 368 (abst.).
Taponen, J; Kulcsár, M; Katila, T; Kátai, L; Huszenicza, G and Rodriguez-Martinez, H (2002). Short estrous cycles and estrous signs after premature ovulations induced with cloprostenol and gonadotropin-releasing hormone in cyclic dairy cows. Theriogenology. 58: 1291-1302.
Taylor, C and Rajamahendran, R (1991). Folicular dynamics, corpus luteum growth and regression in lactating dairy cattle. Can. J. Anim. Sci., 7l: 61-68.
Vojgani, M; Akbarinejad, V and Niasari-Naslaji, A (2013). Administration of eCG on day 6 postpartum could enhance reproductive performance of Holstein dairy cows. Anim. Reprod. Sci., 138: 159-162.
Wiltbank, MC; Gumen, A and Sartori, R (2002). Physiological classification of anovulatory conditions in cattle. Theriogenology. 57: 21-52.