مقایسه تست‌های تشخیصی برای شناسایی بروسلوز گاوی در موارد طبیعی سقط

نوع مقاله : مقاله کامل

نویسندگان

چکیده

تشخیص سریع و دقیق موارد طبیعی سقط گاوی ناشی از بروسلا آبورتوس مستلزم استفاده از حساس‌ترین و قابل اعتمادترین روش‌های تشخیصی است. در مطالعه حاضر، جداسازی باکتری، تکنیک‌های سرولوژی، ظاهری، هیستوپاتولوژی، ایمونوهیستوشیمی و واکنش زنجیره‌ای پلیمراز استفاده شدند. مطالعات شیوع سرمی میزان 86/28% موارد مثبت را با استفاده از ELISA رقابتی نشان دادند. تغییرات هیستوپاتولوژی اغلب در جفت، ریه‌ها، کلیه، کبد و طحال جنین دیده شدند. رنگ‌آمیزی ایمونوهیستوشیمی گونه‌های بروسلا به صورت رنگ قهوه‌ای گرانولر ریز داخل سیتوپلاسمی در تروفوبلاست‌های مقاطع جفت و در مقاطع کبد، ریه، کلیه و طحال مشهود بود. 28 نمونه از 103 نمونه (17 نمونه از محتویات معده، 3 نمونه از کوتیلدون‌های جفت، 2 نمونه از ترشحات واژن و 6 نمونه از بافت‌های جنینی) قطعه 193 جفت بازی اختصاصی جنس بروسلا را تولید کردند. علاوه بر این، پرایمرهای اختصاصی گونه یک قطعه 498 جفت باز را تکثیر کردند که با بروسلا آبورتوس مطابقت داشت. مقایسه تست‌های تشخیصی تایید کرد که واکنش زنجیره‌ای پلیمراز و IHC تست قابل اعتمادی برای تشخیص بروسلوز گاوی در بافت جنین سقط شده و کوتیلدون‌های جفت فراهم کردند، در حالی که سرولوژی مهمترین روش برای ردیابی حیوانات بروسلا مثبت در یک گله است.

کلیدواژه‌ها


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