Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test

Document Type : Short paper


Department of Aerobic Veterinary Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Karadj, Iran


Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas of
Iran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactions
were the main complaint after use of this vaccine. In a previous study, we could improve the vaccine by
alum-precipitation Pasteurella multocida cells and removing the liquid phase. In this study, the amount of
free endotoxin in aluminum-hydroxide and alum-HS vaccines was determined. It was found that endotoxin
level was considerably decreased from 0.22 EU/ml to 0.03 EU/ml after alum-precipitation.