Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants

Document Type : Short paper


1 Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India

2 National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra-282 001, India


Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimation
of prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock with
clinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopy
and blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was substantial.
1262 faecal samples from animals suspected of clinical JD were screened by microscopy in 2009-2010 and
41.3% were positive. Prevalence of MAP was highest in cattle (61.8%), followed by goats (41.3%), sheep
(33.4%) and buffaloes (15.7%). Faecal microscopy was cost effective, easy to adopt and repeatable for the
screening of domestic ruminant population against MAP infection.