TY - JOUR ID - 1833 TI - Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Singh, S. V. AU - Singh, P. K. AU - Gupta, S. AU - Chaubey, K. K. AU - Singh, B. AU - Kumar, A. AU - Singh, A. V. AU - Kumar, N. AD - Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, PO-Farah, Pin-281122, District Mathura, Uttar Pradesh, India AD - National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Tajganj, Agra-282 001, India Y1 - 2013 PY - 2013 VL - 14 IS - 4 SP - 345 EP - 349 KW - paratuberculosis KW - Ziehl Neelsen staining KW - IS900 PCR KW - India DO - 10.22099/ijvr.2013.1833 N2 - Microscopy as “field based test” was compared with IS900 blood PCR for the diagnosis and estimationof prevalence of Mycobacterium avium subspecies paratuberculosis (MAP) in domestic livestock withclinical Johne’s disease (JD). Of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood PCR, respectively. Proportional agreement between “microscopy” and blood PCR was substantial.1262 faecal samples from animals suspected of clinical JD were screened by microscopy in 2009-2010 and41.3% were positive. Prevalence of MAP was highest in cattle (61.8%), followed by goats (41.3%), sheep(33.4%) and buffaloes (15.7%). Faecal microscopy was cost effective, easy to adopt and repeatable for thescreening of domestic ruminant population against MAP infection. UR - https://ijvr.shirazu.ac.ir/article_1833.html L1 - https://ijvr.shirazu.ac.ir/article_1833_d43044a2bd892c904f91ef1040a56ace.pdf ER -