Effect of egg yolk of four avian species on the cryopreserved ram spermatozoa

Document Type : Full paper (Original article)


1 Graduated from College of Agriculture, Shiraz University, Shiraz, Iran

2 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran


Egg yolk is the most commonly used cryoprotective agent for sperm cryopreservation of several species.
The objective of this experiment was to compare the effectiveness of egg yolk from four avian species
(domestic chicken, duck, turkey and pigeon) on sperm quality following cryopreservation of the ram semen.
Ejaculates were collected, using an electroejaculator, from six fertile rams. Samples were diluted in a triscitric
acid-fructose extender containing egg yolk (15%) and glycerol (5%). Diluted samples were cooled
slowly to 5°C over 2 h and equilibrated at that temperature for 2 h. Aliquots of samples were loaded into 0.5
ml straws and frozen in liquid nitrogen vapor for 15 min and stored in liquid nitrogen. Post-thaw progressive
motility, live/dead ratio and acrosomal integrity of 200 sperm per slide stained with eosin-nigrosin and
giemsa, were evaluated at 0, 2 and 4 h after thawing. Pigeon egg yolk had the most cryoprotective effect in
terms of progressive motility, livability and acrosomal integrity (P(Pindicated that pigeon egg yolk might be superior to chicken egg yolk for cryopreservation of ram spermatozoa in tris-citric acid-fructose yolk extender, however, further experiments are needed to evaluate its effects on fertility.