ارزیابی بیوشیمیایی و بافت شناسی جوجه‌های دریافت کننده بتا-هیدروکسی-متیل بوتیرات کلسیم (β-HMB-Ca) در جیره غذایی

نوع مقاله : مقاله کامل

نویسندگان

چکیده

216 عدد جوجه یک روزه نژاد Ross-308 به منظور مطالعه اثر غلظت‌های مختلف بتا-هیدروکسی-متیل بوتیرات کلسیم (β-HMB-Ca) (0، 1/0، 15/0 و 2/0%) بر مقادیر تری-ایدوتیرونین (T3) و تترا-ایدوتیرونین (T4)، آنزیم‌های کبدی [آسپارتات آمینوترانسفراز (AST) و آلانین آمینوترانسفراز (ALT)]، اوریک اسید، پراکساید، مالون دی آلدهاید (MDA)، اسید‌های چرب و همچنین برخی از پارامتر‌های بافت شناسی روده باریک (ضخامت مخاط، ارتفاع ویلی‌ها، ضخامت ویلی‌ها، عمق کریپت‌های اپی تلیال و ارتفاع اپی تلیوم) به 4 گروه مساوی تقسیم شدند. نتایج حاصل از مطالعات بیوشیمی هیچ گونه تغییر معنی‌داری را در مقادیر T3 و T4 و ALT نشان نداد ولی AST در گروه‌های 2 و 3 (P<0.01) و همچنین اوریک اسید در گروه‌های 2 ،3 و 4 (P<0.05) در مقایسه با گروه شاهد کاهش یافته بود. مقادیر پراکساید (PV) و اسید‌های چرب آزاد (FFA) به طور معنی‌داری (به ترتیب P<0.01 و P<0.05) در گروه‌های 2 و 3 نسبت به گروه شاهد کمتر بود. بر اساس نتایج بافت شناسی همه پارامتر‌های مربوط به دوازده در گروه‌های 2 و 3 (P<0.05) و همچنین اکثر پارامتر‌های مربوط به ژژنوم در گروه‌های 2 و 4 به طور معنی‌دار (P<0.05) تغییر کردند. در پایان این مطالعه نشان داد که افزودن β-HMB-Ca به جیره جوجه‌های گوشتی در بازه زمانی 1 الی 35 روزگی موجب بهبود سطوح عملکرد آنزیم‌های کبدی و اسید اوریک سرم، کاهش پارامتر‌های مربوط به اکسیداسیون در کبد و همچنین بهبود عملکرد و بلوغ روده باریک در جوجه‌های گوشتی می‌شود.

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