Efficacy of enoxacin and levofloxacin against Theileria annulata schizont-infected cell line

Document Type : Full paper (Original article)

Authors

1 Division of Veterinary Medicine, Veterinary Clinical Complex (Veterinary Medicine), College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India

2 Livestock Research Station, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

3 Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

4 Department of Clinics (Veterinary Parasitology), College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

5 Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand-388001, Gujarat, India

6 Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

7 Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

8 Department of Livestock Production Management, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar-385506, Gujarat, India

Abstract

Background: Theileriosis is a tick-borne disease that significantly hampers livestock industries in developing nations. Buparvaquone is the only available option for the management of theileriosis. On the other hand, drug use or organism evolution has led to the evolution of resistance over past twenty years. Treatment for theileriosis requires alternative therapeutic approaches. Aims: This work examined the in-vitro effectiveness of enoxacin and levofloxacin compounds against Theileria annulata cultures, targeting the topoisomerase II pathway of organism. Methods: The schizont infected cell line was established from infected cattle blood. The inhibitory effects of both drugs were assessed on T. annulata along with their cytotoxicity and hemolytic activity. Results: Enoxacin and levofloxacin efficiently inhibited 50% of the infection at 1,71,723 µM and 10,084 µM, respectively. Levofloxacin showed encouraging results during the experiment. For enoxacin and levofloxacin, the 50% cytotoxic effects were also measured at 10,529 µM and 10,116 µM, respectively. Enoxacin was safe on bovine red blood cells with an HC50 value of 26,49,031 µM compared to levofloxacin with 19,828 µM value. Conclusion: Levofloxacin showed satisfactory results compared to enoxacin. Further study is required along with other parameters to evaluate and assess the inhibitory activity of enoxacin and levofloxacin.

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Main Subjects

References

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