Phylogenetic analysis of PB2 gene of H9N2 subtype of avian influenza viruses isolated from commercial chickens in Tehran province of Iran during 1998-2001

Document Type : Full paper (Original article)


Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


The PB2 protein of influenza A viruses has been shown as host range determinant. In this study PB2
gene of five H9N2 isolates from Tehran province during the period of 1998-2001 were partially amplified
and sequenced to do phylogenetic study. Iranian isolates could be classified in Eurasian lineage according to
their PB2 gene sequences. The PB2 genes of these isolates are different from those of Middle-East countries
such as the UAE, Pakistan and Saudi Arabia, as well as India, and it is probable that their ancestors have
undergone reassortment with other subtypes. The Nucleotide sequences of their PB2 are highly similar to
each other with 97.5-99.6% homology percentage showing the least mutations in their genes during the
period of 1998 to 2001. The PB2 of the Iranian isolates contain Glutamic acid-(E) in position 627, Aspartic
acid-(D) in 701 and Serine-(S) in 714 as other avian isolates.