Leishmania infantum in an ownership dog (Dogo Argentino) with lymphadenopathy and extensive cutaneous lesions: Hematological, biochemical, microscopical and molecular findings

Document Type : Scientific report

Authors

1 Ph.D. Student in Clinical Pathology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

10.22099/ijvr.2025.51376.7628

Abstract

Background: Leishmania spp. are the cause of a common zoonotic illness. Dogs are the main reservoirs of the parasites, which play a considerable role in infecting humans and other hosts. Case description: A 2-year-old male dog with evident acute skin lesions and ulcerative nodules on the face was referred to a small animal hospital in Mazandaran province, Iran. In order to detect Leishmania parasites, the popliteal lymph node (LN) was sampled for the microscopical examination and the PCR reaction. Also, the hematological and biochemical parameters were measured. Findings/treatment and outcome: Light microscopy on the LN sample stained with Giemsa revealed Leishman bodies inside and outside of macrophages. Laboratory findings showed mild leukocytosis, lymphocytosis, neutrophilia, low hematocrit, hyperglobulinemia, hyperproteinemia, hypoalbuminemia, declined albumin/globulin ratio, and hyperglycemia. The PCR and sequencing results confirmed that Leishmania infantum was the causative agent. Conclusion: Cutaneous leishmaniasis due to L. tropica and L. major is prevalent in humans and dogs in Iran. In this report, a generalized skin disease was evident caused by L. infantum, a viscerotropic parasite species. In addition to cutaneous signs, leukocytosis and anemia concordance with the change in biochemical parameters supported a visceral invasion. Therefore, this report is significant as the cutaneous form of the disease may also imply a complicated visceral illness. In Mazandaran province, the visceral type has been rarely reported. As a result, this form of leishmaniasis in dogs raises concerns about the possibility of zoonotic transmission and may threaten public health.

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References

Almeida, MAO; Jesus, EEV; Sousa-Atta, MLB; Alves, LC; Berne, MEA and Atta, AM (2005). Clinical and serological aspects of visceral leishmaniasis in northeast Brazili dogs naturally infected with Leishmania chagasi. Vet. Parasitol., 127: 227-232.
Amusategui, I; Sainz, A; Rodriguez, F and Tesouro, MA (2003). Distribution and relationships betweenclinical and biopathological parameters in canine leishmaniasis. Eur. J. Epidemiol., 18: 147-156.
Asgari, Q; Fakhar, M; Motazedian, MH; Cheraqali, F and Banimostafavi, E (2007). Visceral leishmaniasis, an alarming rate of misdiagnosis. Iran. Red Crescent Med. J., 95: 45-47.
Bamorovat, M; Sharifi, I; Dabiri, S; Mohammadi, MA; Harandi, MF; Mohebali, M; Aflatoonian, MR and Keyhani, A (2015). Leishmania tropica in stray dogs in southeast Iran. Iran. J. Public Health. 44: 1359-1366.
Baneth, G; Nachum-Biala, Y; Adamsky, O and Gunther, I (2022). Leishmania tropica and Leishmania infantum infection in dogs and cats in central Israel. Parasit. Vectors. 15: 147.
Baneth, G; Nachum-Biala, Y; Shabat Simon, M; Brenner, O; Gaier, S; Rojas, A and Yasur-Landau, D (2016). Leishmania major infection in a dog with cutaneous manifestations. Parasit. Vectors. 9: 246.
Baneth, G; Yasur-Landau, D; Gilad, M and Nachum-Biala, Y (2017). Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology. Parasit. Vectors. 10: 113.
Bi, K; Chen, Y; Zhao, S; Kuang, Y and John Wu, CH (2018). Current visceral leishmaniasis research: a research review to inspire future study. Biomed. Res. Int., 2018: 9872095.
Bogdan, C; Schönian, G; Banuls, AL; Hide, M; Pratlong, F; Lorenz, E; Rollinghoff, M and Mertens, R (2001). Visceral leishmaniasis in a German child who had never entered a known endemic area: case report and review of the literature. Clin. Infect. Dis., 32: 302-306.
Ciaramella, P and Corona, M (2003). Canine leishmaniasis: clinical and diagnostic aspects. Compend. Contin. Educ. Vet., 25: 358-369.
da Costa-Val, AP; Cavalcanti, RR; de Figueiredo Gontijo, N; Michalick, MSM; Alexander, B; Williams, P and Melo, MN (2007). Canine visceral leishmaniasis: relationships between clinical status, humoral immune response, haematology and Lutzomyia (Lutzomyia) longipalpis infectivity. Vet. J., 174: 636-643. https://doi. org/10.1016/j.tvjl.2006.11.006.
Dantas-Torres, F (2007). The role of dogs as reservoirs of Leishmania parasites, with emphasis on Leishmania (Leishmania) infantum and Leishmania (Viannia) braziliensis. Vet. Parasitol., 149: 139-146.
de Almeida, ME; Steurer, FJ; Koru, O; Herwaldt, BL; Pieniazek, NJ and da Silva, AJ (2011). Identification of Leishmania spp. by molecular amplification and DNA sequencing analysis of a fragment of rRNA internal transcribed spacer 2. J. Clin. Microbiol., 49: 3143-3149.
de Andrade, HM; Reis, AB; dos Santos, SL; Volpini, ÂC; Marques, MJ and Romanha, AJ (2006). Use of PCR-RFLP to identify Leishmania species in naturally-infected dogs. Vet. Parasitol., 140: 231-238.
Dias, EL; Batista, ZDS; Guerra, RMSNC; Calabrese, KDS; Lima, TB and Abreu-Silva, AL (2008). Canine visceral leishmaniasis (CVL): seroprevalence, clinical, hemato-logical and biochemical findings of dogs naturally infected in an endemic area of São José de Ribamar municipality, Maranhão state, Brazil. Ciência. Anim. Bras., 9: 740-745.
Fakhar, M; Derakhshani-nia, M; Gohardehi, S; Karamian, M; Hezarjaribi, HZ; Mohebali, M; Akhoundi, B and Sharbatkhori, M (2022). Domestic dogs carriers of Leishmania infantum, Leishmania tropica and Crithidia fasciculata as potential reservoirs for human visceral leishmaniasis in northeastern Iran. Vet. Med. Sci., 8: 2329-2336.
Fakhar, M and Rahmati, B (2011). Visceral leishmaniasis in Mazandaran province and review on its current situation in Iran. J. Babol Univ. Med. Sci., 13: 68-75.
Fakhar, M; Rahmati, B; Gohardehi, S; Mohebali, M; Akhoundi, B; Sharif, M and Ali Mahdavi, S (2011). Molecular and seroepidemiological survey of visceral leishmaniasis among humans and domestic dogs in Mazandaran Province, North of Iran. Iran. J. Parasitol., 6: 51-59.
Foglia Manzillo, V; Di Muccio, T; Cappiello, S; Scalone, A; Paparcone, R; Fiorentino, E; Gizzarelli, M; Gramiccia, M; Gradoni, L and Oliva, G (2013). Prospective study on the incidence and progression of clinical signs in naïve dogs naturally infected by Leishmania infantum. PLoS Negl. Trop. Dis., 7: e2225.
Geisweid, K; Mueller, R; Sauter-Louis, C and Hartmann, K (2012). Prognostic analytes in dogs with Leishmania infantum infection living in a non-endemic area. Vet. Rec., 171: 399-399.
Hajjaran, H; Mohebali, M; Zarei, Z and Edrissian, GH (2007). Leishmania tropica: another agent of canine visceral leishmaniasis in Iran. Iran. J. Public Health. 36: 85-88.
Ikeda-Garcia, FA; Ciarlini, PC; Lopes, RS; Marques, FJ; Bomfim, SRM; Lima, VMFD; Perri, SHV and Marcondes, M (2008). Hematological evaluation of dogs naturally infected by Leishmania (Leishmania) chagasi submitted to treatment with meglumine antimoniate. Braz. J. Vet. Res. Anim. Sci., 45: 68-74.
Jafari Shourijeh, S; Tamadon, A; Fakhar, M; Motazedian, MH; Bigham, A and Asghari, Q (2006). First report of visceral leishmaniasis in a German shepherd dog in Fars Province, Southern Iran. Comp. Clin. Path., 15: 267-269.
Koutinas, AF; Polizopoulou, ZS; Saridomichelakis, MN; Argyriadis, D; Fytianou, A and Plevraki, KGZ (1999). Clinical considerations on canine visceral leishmaniasis in Greece: a retrospective study of 158 cases (1989-1996). J. Am. Anim. Hosp. Assoc., 35: 376-383.
Kumar, S; Stecher, G; Li, M; Knyaz, C and Tamura, K (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35: 1547-1549. https://doi.org/10.1093/molbev/msy096.
Marcelino, AP; de Souza Filho, JA; e Bastos, CDV; Ribeiro, SR; Medeiros, FAC; Reis, IA; da Rocha Lima, ACVM; Barbosa, JR; Paz, GF and Gontijo, CMF (2020). Comparative PCR-based diagnosis for the detection of Leishmania infantum in naturally infected dogs. Acta Trop., 207: 105495.
Mohammadiha, A; Haghighi, A; Mohebali, M; Mahdian, R; Abadi, AR; Zarei, Z; Yeganeh, F; Kazemi, B; Taghipour, N; Akhoundi, B; Barati, M and Mahmoudi, MR (2013). Canine visceral leishmaniasis: a comparative study of real-time PCR, conventional PCR, and direct agglutination on sera for the detection of Leishmania infantum infection. Vet. Parasitol., 192: 83-90.
Mohebali, M; Edrissian, G; Akhoundi, B; Shirzadi, M; Hassanpour, G; Behkar, A; Rassi, Y; Hajjaran, H; Keshavarz, H; Gouya, MM; Arshi, S; Zeinali, M; Zarei, Z; Sharifi, I and Kakooei, Z (2023). Visceral leishmaniasis in Iran: An update on epidemiological features from 2013 to 2022. Iran. J. Parasitol., 18: 279-293.
Mohebali, M; Edrissian, GH; Nadim, A; Hajjaran, H;
Akhoundi, B; Hooshmand, B; Zarei, Z; Arshi, Sh; Mirsamadi, N; Manouchehri Naeini, K; Mamishi, S; Sanati, AA; Moshfe, AA; Charehdar, S and Fakhar, M (2006). Application of direct agglutination test (DAT) for the diagnosis and seroepidemiological studies of visceral leishmaniasis in Iran. Iran. J. Parasitol., 1: 15-25.
Mohebali, M; Hajjaran, H; Hamzavi, Y; Mobedi, I; Arshi, S; Zarei, Z; Akhoundi, B; Naeini, KM; Avizeh, R and Fakhar, M (2005). Epidemiological aspects of canine visceral leishmaniosis in the Islamic Republic of Iran. Vet. Parasitol., 129: 243-251.
Nicolato, RDC; Abreu, RTD; Roatt, BM; Aguiar-Soares, RDDO; Reis, LES; Carvalho, MDG; Carneiro, CM; Giunchetti, RC; Bouillet, LEM; Lemos, DS and Coura-Vital, W (2013). Clinical forms of canine visceral leishmaniasis in naturally Leishmania infantum-infected dogs and related myelogram and hemogram changes. PloS One. 8: e82947.
Oliva, G; Scalone, A; Foglia Manzillo, V; Gramiccia, M; Pagano, A; Di Muccio, T and Gradoni, L (2006). Incidence and time course of Leishmania infantum infections examined by parasitological, serologic, and nested-PCR techniques in a cohort of naive dogs exposed to three consecutive transmission seasons. J. Clin. Microbiol., 44: 1318-1322.
Paiva-Cavalcanti, M; Regis-da-Silva, CG and Gomes, YM (2010). Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. J. Venom. Anim. Toxins Incl. Trop. Dis., 16: 537-542.
Paltrinieri, S; Gradoni, L; Roura, X; Zatelli, A and Zini, E (2016). Laboratory tests for diagnosing and monitoring canine leishmaniasis. Vet. Clin. Pathol., 45: 552-578.
Paltrinieri, S; Solano-Gallego, L; Fondati, AL; ubas, G; Gradoni, L; Castagnaro, M; Crotti, A; Maroli, M; Oliva, G; Roura, X and Zini, E (2010). Guidelines for diagnosis and clinical classification of leishmaniasis in dogs. J. Am. Vet. Med. Assoc., 236: 1184-1191.
Pouya, Y (1949). Study of visceral leishmaniasis in Gilan and Mazadndran provinces of Iran. J. Med. Fac. Tehran. 7: 359-361.
Proverbio, D; Spada, E; Bagnagatti de Giorgi, G; Perego, R and Valena, E (2014). Relationship between Leishmania IFAT titer and clinicopathological manifestations (clinical score) in dogs. Biomed. Res. Int., 2014: 412808.
Reithinger, R and Davies, CR (1999). Is the domestic dog (Canis familiaris) a reservoir host of American cutaneous leishmaniasis? A critical review of the current evidence. Am. J. Med. Hyg., 61: 530-541.
Ribeiro, RR; Silva, SMD; Fulgêncio, GDO; Michalick, MSM and Frézard, FJG (2013). Relationship between clinical and pathological signs and severity of canine leishmaniasis. Rev. Bras. Parasitol., 22: 373-378.
Saridomichelakis, MN and Koutinas, AF (2014). Cutaneous involvement in canine leishmaniosis due to Leishmania infantum (syn. L. chagasi). Vet. Dermatol., 25: 61-e22.
Shokri, A; Fakhar, M and Teshnizi, SH (2017). Canine visceral leishmaniasis in Iran: A systematic review and meta-analysis. Acta Trop., 165: 76-89.
Solano-Gallego, L; Koutinas, A; Miró, G; Cardoso, L; Pennisi, MG; Ferrer, L; Bourdeau, P; Oliva, G and Baneth, G (2009). Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis. Vet. Parasitol., 165: 1-18.

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