Detection of Brucella infection in raw milk of livestock in Famenin, West part of Iran

Document Type : Short paper

Authors

1 Infectious Diseases Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; Infectious Ophthalmologic Research Center, Imam Khomeini Hospital Clinical Research Development Unit, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Department of Laboratory Sciences, Central Veterinary Laboratory, Iranian Veterinary Organization (IVO), Hamedan Veterinary Office, Hamedan, Iran

3 Department of Brucellosis, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Infectious Diseases Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

5 MSc in Health Education, Deputy of Health, Hamadan University of Medical Sciences, Hamadan, Iran

6 MSc in Statistics, Faculty of Health, Hamadan University of Medical Sciences, Hamadan, Iran

7 MSc in Microbiology, Department of Microbiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran

8 Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran

9 Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

Abstract

Background: Timely diagnosis of brucellosis is the starting point for effective programs to control brucellosis in humans and animals. Aims: This study aimed to detect Brucella infection in milk samples from livestock in Famenin, an endemic region of western Iran, using a milk ring test and molecular techniques. Methods: In this cross-sectional study, 738 raw milk samples were randomly collected from cattle, sheep, and goats. Milk samples were screened using the milk ring test (MRT). In addition, polymerase chain reaction (PCR) method was applied to detect Brucella spp. in all MRT-positive samples. DNA from the milk samples was extracted and used for PCR using the BCSP31 and IS711 loci. Results: Of the samples tested using MRT, 46 (6.23%, 95% CI: 2.83-9.63%) yielded positive results. Of the 46 seropositive samples, 42 (91.30%) were from sheep and 4 (8.70%) were from goats, while no bovine samples had positive MRT results. PCR analysis confirmed that 78.26% (36/46) of MRT-positive samples belonged to the genus Brucella. Furthermore, 83.33% (30/36) of the confirmed samples were identified as B. melitensis, while 16.66% (6/36) were identified as B. abortus. Conclusion: The results obtained from MRT evaluation of milk samples did not align entirely with the findings of the molecular examinations. The PCR method has minimal biological contamination and high sensitivity and accuracy, especially for determining Brucella species. Raw milk should be routinely assessed for Brucella contamination. This work is necessary to identify hidden infections and break the chain of transmission of brucellosis.

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Main Subjects

References

Abd El-Rahim, I and Asghar, A (2014). Brucellosis in ruminant animals and their close contact humans in western region of Saudi Arabia in 2012. Assiut Vet. Med. J., 60: 1-6.
Adabi, M; Karami, M; Keramat, F; Alikhani, MY and Bakhtiari, S (2021). Serological and molecular investigation of human brucellosis in participants of Famenin brucellosis cohort study, Hamadan, Iran. Iran. J. Microbiol., 13: 319-324.
Adabi, M; Khazaiee, S; Sadeghi-Nasab, A; Alamian, S; Arabestani, MR; Valiei, Z and Gharekhani, J (2022). Brucellosis in livestock: First study on seroepidemiology, risk factors, and preventive strategies to manage the disease in Famenin, Iran. Vet. World. 15: 2102-2110.
Aggad, H and Boukraa, L (2006). Prevalence of bovine and human brucellosis in western Algeria: comparison of screening tests. East. Mediterr. Health J., 12: 119-128.
Akinyemi, KO; Fakorede, CO; Amisu, KO and Wareth, G (2022). Human and animal brucellosis in Nigeria: A systemic review and meta-analysis in the last twenty-one years (2001-2021). Vet. Sci., 9: 384-407.
Al-Afifi, AH; Alewy Almashhadany, D; Al-Azazi, ASH; Khalaf, AM; Naji Ahmed Odhah, M and Al-Gabri, NA (2022). Prevalence of Brucella spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen. Ital. J. Food. Saf., 11: 10370-10376.
Alamian, S; Amiry, K; Bahreinipour, A; Etemadi, A; Tebianian, M; Mehrabadi, MHF and Dadar, M (2021). Brucella species circulating in rural and periurban dairy cattle farms: a comparative study in an endemic area. Trop. Anim. Health Prod., 53: 200-208.
Al Dahouk, S and Nöckler, K (2011). Implications of laboratory diagnosis on brucellosis therapy. Expert. Rev. Anti. Infect. Ther., 9: 833-845.
Aliyev, J; Alakbarova, M; Garayusifova, A; Omarov, A; Aliyeva, S; Fretin, D and Godfroid, J (2022). Identification and molecular characterization of Brucella abortus and Brucella melitensis isolated from milk in cattle in Azerbaijan. B.M.C. Vet. Res., 18: 71-79.
Babaoglu, UT; Ogutucu, H; Demir, G; Sanli, D; Babaoglu, AB and Oymak, S (2018). Prevalence of brucella in raw milk: An example from Turkey. Niger. J. Clin. Pract., 21: 907-911.
Bahmani, N and Bahmani, A (2022). A review of brucellosis in the Middle East and control of animal brucellosis in an Iranian experience. Rev. Med. Microbiol., 33: 63-69.
Bazrgari, N; Garoosi, GA and Dadar, M (2020). Genetic diversity and phylogenetic relationship of clinical isolates of Brucella melitensis based on gene polymorphism of β subunit of RNA polymerase (rpoB) gene in Iran. Iran. J. Med. Microbiol., 14: 425-440.
Béjaoui, A; Ben Abdallah, I and Maaroufi, A (2022). Brucella spp. contamination in artisanal unpasteurized dairy products: An emerging foodborne threat in Tunisia. Foods. 11: 2269-2277.
Bennett, NJB and Bronze, MS (2021). Brucellosis. Accessed on 22 April 2021, Available from: https://emedicine. medscape.com/article/213430-print.
Cadmus, SI; Adesokan, HK and Stack, J (2008). The use of the milk ring test and rose bengal test in brucellosis control and eradication in Nigeria. J. S. Afr. Vet. Assoc., 79: 113-115.
Dadar, M; Alamian, S; Behrozikhah, AM; Yazdani, F; Kalantari, A; Etemadi, A and Whatmore, AM (2019a). Molecular identification of Brucella species and biovars associated with animal and human infection in Iran. Vet. Res. Forum., 10: 315-321.
Dadar, M; Shahali, Y and Fakhri, Y (2021). Brucellosis in Iranian livestock: A meta-epidemiological study. Microb. Pathog., 155: 1-7.
Dadar, M; Shahali, Y and Whatmore, AM (2019b). Human brucellosis caused by raw dairy products: A review on the occurrence, major risk factors and prevention. Int. J. Food Microbiol., 292: 39-47.
Garshasbi, M; Ramazani, A; Sorouri, R; Javani, S and Moradi, S (2014). Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran. Braz. J. Microbiol., 45: 533-538.
Gharekhani, J; Yakhchali, M; Afshari, A and Adabi, M (2021). Herd-level contamination of Neospora caninum, Toxoplasma gondii and Brucella in milk of Iranian dairy
farms. Food Microbiol., 100: 1-8.
Keramat, F; Karami, M; Alikhani, MY; Bashirian, S; Moghimbeigi, A and Adabi, M (2019). Cohort profile: Famenin brucellosis cohort study. J. Res. Health Sci., 19: 453-457.
Keramat, F; Karami, M; Alikhani, MY; Bashirian, S; Moghimbeigi, A; Hashemi, SH; Mamani, M; Shivapoor, Z; Bosilkovski, M and Adabi, M (2020). Seroprevalence of brucellosis among general population in Famenin city, Western Iran in 2016: The Famenin Brucellosis Cohort Study. Arch. Iran. Med., 23: 749-756.
Khan, TI; Ehtisham ul Haque, S; Waheed, U; Khan, I; Younus, M and Ali, S (2018). Milk indirect-ELISA and milk ring test for screening of brucellosis in buffaloes, goats and bulk tank milk samples collected from two districts of Punjab. Pak. Vet. J., 38: 105-108.
Kolo, FB; Adesiyun, AA; Fasina, FO; Potts, A; Dogonyaro, BB; Katsande, CT and Van Heerden, H (2021). A retrospective study (2007-2015) on brucellosis seropositivity in livestock in South Africa. Vet. Med. Sci., 7: 348-356.
Mantur, B; Parande, A; Amarnath, S; Patil, G; Walvekar, R; Desai, A; Parande, M; Shinde, R; Chandrashekar, M and Patil, S (2010). ELISA versus conventional methods of diagnosing endemic brucellosis. Am. J. Trop. Med. Hyg., 83: 314-318.
Marianelli, C; Martucciello, A; Tarantino, M; Vecchio, R; Iovane, G and Galiero, G (2008). Evaluation of molecular methods for the detection of Brucella species in water buffalo milk. J. Dairy Sci., 91: 3779-3786.
Mohamand, N; Gunaseelan, L; Sukumar, B and Kannan, P (2014). Milk Ring Test for spot identification of Brucella abortus infection in single cow herds. J. Adv. Vet. Anim. Res., 1: 70-72.
Mohammed, M; Shigidy, MT and Juboori, AYA (2013). Sero-prevalence and epidemiology of brucellosis in camels, sheep and goats in Abu Dhabi Emirate. Int. J. Anim. Vet. Adv., 5: 82-86.
Mustafa, YS; Awan, FN and Khalid, H (2011). Prevalence of brucellosis in sheep and goat. Sci. Int. (Lahore), 23: 211-212.
Nofal, AS; El-Leboudy, AA; El-Makarem, HSA and El-Rahman, AHA (2017). Prevalence of Brucella organism in milk and serum samples of some lactating dairy animals. Alexandria. J. Vet. Sci., 55: 19-24.
Shakir, R (2021). Brucellosis. J. Neurol. Sci., 420: 1-5.
Sharifi, H; Tabatabaei, S; Rashidi, H; Kazeminia, S; Sabbagh, F; Khajooei, P; Karamouzian, M; Nekouei, O; Adeli Sardooei, M and Leontides, L (2014). A cross-sectional study of the seroprevalence and flock-level factors associated with ovine and caprine brucellosis in southeastern Iran. Iran. J. Vet. Res., 15: 370-374.
World Health Organization (2022). Brucellosis. Accessed: 2022, Available: https://www.who.int/news-room/fact-sheets/detail/brucellosis.
ZareBidaki, M; Allahyari, E; Zeinali, T and Asgharzadeh, M (2022). Occurrence and risk factors of brucellosis among domestic animals: an artificial neural network approach. Trop. Anim. Health Prod., 54: 62-71.
Zhou, K; Wu, B; Pan, H; Paudyal, N; Jiang, J; Zhang, L; Li, Y and Yue, M (2020). One health approach to address zoonotic brucellosis: A spatiotemporal associations study between animals and humans. Front. Vet. Sci., 7: 521-530.

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