شناسایی فنوتیپی و ژنوتیپی گونه‌های استافیلوکوکوس کواگولاز مثبت مقاوم به چند دارو جدا شده از پیودرمای سگ

نوع مقاله : مقاله کامل

نویسندگان

چکیده

پیشینه: درمان عفونت‌های پوستی در سگ‌ها به دلیل مقاومت آنتی‌بیوتیکی چالش برانگیز است چرا که همراه با زمان بهبودی طولانی‌تر و نیاز به آنتی‌بیوتیک‌های قوی‌تر است. هدف: این مطالعه به منظور تعیین شیوع مقاومت ضد میکروبی در ایزوله‌های استافیلوکوکوس کواگولاز مثبت از عفونت پیودرما در سگ انجام شد. این مطالعه همچنین به شناسایی ایزوله‌های دارای مقاومت به متی‌سیلین و مقاومت چند دارویی نیز پرداخت. روش کار: در مجموع 73 استافیلوکوک کواگولاز مثبت از درجات مختلف پیودرمای سگ جداسازی شد. نمونه‎ها از نظر وجود گونه‌های استافیلوکوک مورد بررسی قرار گرفتند. با شیوع آلودگی و مقاومت در برابر آنتی‌بیوتیک‌ها با استفاده از پروفایل ضد میکروبی، حداقل غلظت مهاری (MIC) و PCR بر روی باکتری‌های جدا شده مورد بررسی قرار گرفت. نتایج: از 75 ایزوله باکتری شناسایی شده، 73 ایزوله به عنوان گونه استافیلوکوکوس توسط PCR تایید شد. درصد بالاتری از مقاومت آنتی بیوتیکی در برابر پنی سیلین-G (27/46%) و پس از آن آموکسی کلاو (81/38%)، انروفلوکساسین (84/32%)، سفپودوکسیم، اکسی تتراسایکلین (هر کدام 36/28%)، لووفلوکساسین (86/26%)، و کوتریموکسازول (39/22%) مشاهده شد. 29 جدایه (15/49%) S. pseudintermedius، سه جدایه (50%) S. schleiferi subsp coagulans، و دو جدایه (100%) استافیلوکوکوس اورئوس مقاومت چند دارویی را نشان دادند. با این حال، یک جدایه (49/1%) S. pseudintermedius مقاومت موپیروسین در سطح پایین را در آزمون E نشان داد. همچنین، 12 نمونه (34/20%) مقاوم به متی‌سیلین استافیلوکوک کاذب (MRSP)، یک نمونه (67/16%) مقاوم به متی‌سیلین S. schleiferi subsp coagulans، (MRSS) و یک نمونه (50%) استافیلوکوکوس اورئوس مقاوم به متی‌سیلین (MRSA) با استفاده از PCR گزارش شدند. نتیجه‌گیری: این مطالعه به درک افزایش سطح و الگوی مقاومت در استافیلوکوکوس‌های کواگولاز مثبت جدا شده از انواع مختلف موارد پیودرمای سگ کمک می‌کند.

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موضوعات


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