Evaluation of the efficiency of estrus synchronization protocols combined with natural service and ultrasonography on ewe reproductive performance during non-breeding season

Document Type : Short paper

Author

Unit of Teaching Veterinary Clinical Complex, Faculty of Veterinary and Animal Sciences, RGSC-Banaras Hindu University, Mirzapur (UP)-231001, India

Abstract

Background: Estrus synchronization is an important assisted reproductive technology to improve the reproductive performance in ewes. Various protocols have been used with variable success rates, however; literature regarding field applicable estrus synchronization is meagre. Aims: The present study was designed with the aim to evaluate the estrus synchronization protocols on reproductive performance in ewes using different hormones. Methods: Experimental ewes were divided randomly into three groups (n=15). Ewes of all groups received intravaginal sponge for 12 days. Subsequently, NP4-GnRH and NP4-eCG groups received 8 µg of buserelin acetate or 200 IU of eCG intramuscularly, respectively on day 12 whereas NP4-Insulin group received insulin 0.2 IU/kg body weight subcutaneously for three consecutive days started on the day of sponge removal. Estrus detection commenced 24 h after sponge removal in NP4-GnRH and NP4-eCG groups and 24 h following last injection of insulin in NP4-Insulin group. The ewes in estrus were separated and pen mated. The conception rate was determined by ultrasonography. Results: The estrus response and conception rates were 71.43, 92.86 and 53.85%, and 70.00, 84.61, and 71.43%, respectively in NP4-GnRH, NP4-eCG, and NP4-Insulin groups. The lambing rates were the same as the conception rates. The single and multiple birth rates were 71.41, 36.36 and 60.0%, and 28.57, 63.64, and 40.0% whereas prolificacy was 128.57, 190.91, and 140.00%, respectively in NP4-GnRH, NP4-eCG, and NP4-Insulin groups. Conclusion: In conclusion, the estrus synchronization protocol including intravaginal progesterone sponge and eCG was found to be more effective under field conditions.

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References

Alvarez, M; Anel-Lopez, L; Boixo, JC; Chamorro, C; Neila-Montero, M and Montes-Garrido, R (2019). Current challenges in sheep artificial insemination: a particular insight. Reprod. Domest. Anim., 54: 32-40.
Ciornei, SG; Drugociu, D; Ciornei, L and Roşca, P (2022). Ovarian response to P4-PGF-FSH treatment in Suffolk sheep and P4-PGF-PMSG synchronization in cross-bred ewes, for IVD and ET protocol. Vet. Med. Sci., 8: 726-734.
Cosentino, IO; Balaro, MFA; Arashiro, EKN; Santos, JDR; Carvalho, AB; da, S; Clariget, RP; Ungerfeld, R and Brandao, FZ (2019). Hormonal protocols for early resynchronization of ovulation in ewes: The use of progestagens, eCG, and inclusion of early pregnancy diagnosis with color Doppler ultrasound. Theriogenology. 133: 113-118.
Das, GK; Naqvi, SMK; Gulyani, R; Pareek, SR and Mittal, JP (2000). Effect of two doses of progesterone on estrus response and fertility in acycling crossbred Bharat Merino ewes in a semi-arid tropical environment. Small Rumin. Res., 37: 159-163.
De, K; Kumar, D; Balaganur, K; Gulyani, R and Naqvi, SMK (2016). Effect of breeding season on fertility of sheep following estrus synchronization and fixed-time artificial insemination under field conditions in semi-arid tropical region. Biol. Rhythm Res., 47: 787-795. doi: 10.1080/ 09291016.2016.1197497.
De, K; Kumar, D; Sethi, D; Gulyani, R and Naqvi, SMK (2015). Estrus synchronization and fixed time artificial insemination in sheep under field conditions of a semi-arid tropical region. Trop. Anim. Health Prod., 47: 469-472.
Dias, J; Miranda, V; Oliveira, F; Junior, SV; Haas, C; Costa, V; Lucia Jr, T; Vieira, A; Corcini, C and Gasperin, B (2020). Treatment with eCG and hCG to induce onset of estrous cycles in ewes during the non-breeding season: Effects on follicular development and fertility. Anim. Reprod. Sci., 212: 106232. https://doi.org/ 10.1016/j.anireprosci.2019.106232.
Gong, JG; McBride, D; Bramley, TA and Webb, R (1994). Effects of recombinant bovine somatotrophin, insulin-like growth factor-I and insulin on bovine granulosa cell steroidogenesis in vitro. J. Endocrinol., 143: 157-164.
Hameed, N; Khan, MI and Andrabi, SMH (2021). Approaches of estrous synchronization in sheep: developments during the last two decades: a review. Trop. Anim. Health Prod., 53: 485. doi: 10.1007/s11250-021-02932-8.
Husein, MQ; Ababneh, MM and Abu-Ruman, DS (2007). The effects of short or long term FGA treatment with or without eCG on reproductive performance of ewes bred out-of-season. Am. J. Anim. Vet. Sci., 2: 23-28.
Karaca, F; Ataman, MB and Oyan, KC (2009). Synchronization of estrus with short- and long-term progestagen treatments and the use of GnRH prior to short-term progestagen treatment in ewes. Small Ruminant Res., 81: 185-188.
Majumdar, AC; Karche, SD; Tyagi, S and Taru Sharma, G (1997). Effect of pretreatment with hCG and estradiol-17b on superovulation and embryo recovery in goats. Theriogenology. 47: 176. doi: 10.1016/s0093-691x(97) 82303-5.
Martinez-Ros, P; Rios-Abellan, A and Gonzalez-Bulnes, A (2019). Influence of progesterone-treatment length and eCG administration on appearance of estrous behavior, ovulatory success and fertility in sheep. Animals. 9: 9. doi: 10.3390/ani9010009.
Mura, M; Luridiana, S; Pulinas, L; Bizzarri, D; Cosso, G and Carcangiu, V (2019). Melatonin treatment and male replacement every week on the reproductive performance in Sarda sheep breed. Theriogenology. 135: 80-84.
Nakafeero, A; Hassen, A and Lehloenya, K (2020). Investigation of ram effect and eCG usage in progesterone based oestrous synchronization protocols on fertility of ewes following fixed time artificial insemination. Small Rumin. Res., 183: 106034. https://doi.org/10.1016/j. smallrumres.2019.106034.
Oliveira, ME; Ayres, H; Oliveira, LG; Barros, FF; Oba, E; Bicudo, SD; Bartlewski, PM; Fonseca, JF and Vicente, WR (2016). Effects of season and ovarian status on the outcome of long-term progesterone-based estrus synchronization protocols and ovulatory follicle development in Santa Ines ewes under subtropical conditions. Theriogenology. 85: 452-460.
Peters, AR; Ward, SJ; Warren, MJ; Gordon, PJ; Mann, GE and Webb, R (1999). Ovarian and hormonal responses of cows to treatment with an analogue of gonadotrophin releasing hormone and prostaglandin F2. Vet. Rec., 144: 343-346.
Quintero-Elisea, JA; Macias-Cruz, U; Alvarez-Valenzuela, FD; Correa-Calderon, A; Gonzalez-Reyna, A; Lucero-Magana, FA; Soto-Navarro, SA and Avendano-Reyes, L (2011). The effects of time and dose of pregnant mare serum gonadotropin (PMSG) on reproductive efficiency in hair sheep ewes. Trop. Anim. Health Prod., 43: 1567-1573.
Ramoun, AA; Osman, KT; Darwish, SA; Karen, AM and Gamal, MH (2007). Effect of pretreatment with insulin on the response of buffaloes with inactive ovaries to gonadotrophin-releasing hormone agonist treatment in summer. Reprod. Fertil. Dev., 19: 351-355.
Reinoso-Pelaez, EL; Saura, M; Gonzalez-Recio, O; Gonzalez, C; Fernandez, A; Peiro-Pastor, R; Lopez-Garcia, A; Saborio-Montero, A; Calvo, JH; Ramon, M and Serrano, M (2023). Impact of oestrus synchronization devices on ewes vaginal microbiota and artificial insemination outcome. Front. Microbiol., 14: 1063807. doi: 10.3389/fmicb.2023.1063807.
Roy, F; Combes, B; Vaiman, D; Cribiu, EP; Pobel, T; Deletang, F; Combarnous, Y; Guillou, F and Maurel, MC (1999). Humoral immune response to equine chorionic gonadotropin in ewes: association with major histocompatibility complex and interference with subsequent fertility. Biol. Reprod., 61: 209-218.
Santos-Jimenez, Z; Martinez-Herrero, C; Encinas, T; Martinez-Ros, P and Gonzalez-Bulnes, A (2020). Comparative efficiency of oestrus synchronization in sheep with progesterone/eCG and progesterone/GnRH during breeding and non-breeding season. Reprod. Dom. Anim., 55: 882-884.
Sarath, T; Mehrotra, S; Agarwal, SK; Varshney, VP; Hoque, M; Shankar, U and Singh, SK (2008). Effect of insulin administration on ovarian function and estrus induction in acyclic goats. Anim. Reprod. Sci., 108: 216-225.
Sen, U and Onder, H (2016). The effect of estrus synchronization programmes on parturition time and some reproductive characteristics of Saanen goats. J. Appl. Anim. Res., 44: 376-379.
Suguna, K; Mehrotra, S; Agarwal, SK; Hoque, M; Shanker, U; Singh, SK and Varshney, VP (2009). Effect of exogenous insulin administration on ovarian function, embryo/fetal development during pregnancy in goats. Anim. Reprod. Sci., 111: 202-213.
Titi, HH; Kridli, RT and Alnimer, MA (2010). Estrus
synchronization in sheep and goats using combinations of GnRH, progestagen and prostaglandin F2α. Reprod. Dom. Anim., 45: 594-599.
Vilarino, M; Rubianes, E and Menchaca, A (2013). Ovarian responses and pregnancy rate with previously used intravaginal progesterone releasing devices for fixed-time artificial insemination in sheep. Theriogenology. 79: 206-210.
Vinoles, C; Forsberg, M; Banchero, G and Rubianes, E (2001). Effect of long term and short-term progestagen treatment on follicular development and pregnancy rate in cyclic ewes. Theriogenology. 55: 993-1004.
Webb, R; Gong, JG and Rusbridge, SM (1992). Control of ovarian function in cattle. J. Reprod. Fert., 45: 141-156.
Wildeus, S (2000). Current concepts in synchronization of estrus: Sheep and goats. J. Anim. Sci., 77: 1-14.
Yadav, V; Chandolia, RK; Ranga, LC; Bisla, A; Saini, G; Dutt, R; Singh, G; Patil, S and Kumar, A (2021). Estrus synchronization to combat reproductive seasonality in crossbred ewes. Haryana Vet., 60: 47-50.
Yu, X; Bai, Y; Yang, J; Zhao, X; Zhang, L and Wang, J (2022). Comparison of five protocols of estrous synchronization on reproductive performance of Hu sheep. Front. Vet. Sci., 9: 843514. doi: 10.3389/fvets.2022. 843514.
Yu, XJ; Wang, J and Bai, YY (2019). Estrous synchronization in ewes: The use of progestogens and prostaglandins. Acta Agric. Scand., Section A, Animal Science. doi: 10.1080/09064702.2019.1674373.

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