Detection of the Mycobacterium avium complex in dogs with lymphadenitis

Document Type : Full paper (Original article)

Authors

1 MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

2 Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

3 Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

4 Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India

Abstract

Background: Mycobacterium avium complex (MAC) is connected to human immunosuppressive diseases, including HIV-AIDS, and may pose a zoonotic threat. MAC causes lymphadenopathy in children, respiratory infection in adults, and generalized infection in immunocompromised individuals. Infection with nontuberculous Mycobacteria (NTM) in humans is now primarily brought on by MAC. Recently, MAC members have emerged as pathogenic organisms for animals and humans. While dogs are generally resistant to mycobacterial infections, there have been some cases of infection that result in systemic or disseminated diseases. The organisms can be transmitted to dogs through oral contact, and their faeces can be a possible source of infection for dog owners. It is important to note that this ailment is zoonotic, especially if infected pet dogs are in prolonged contact with their humans. Aims: The study was planned to demonstrate the occurrence of MAC organisms and other Mycobacteria in dogs associated with lymphadenopathy cases with special emphasis on lymphadenitis. Methods: A total of 123 samples (100 lymph node aspirates, 15 lymph node tissues, and 8 blood samples) from 83 dogs suspected of lymphadenitis accompanied by gastroenteritis, chronic skin infections, immunosuppression, chronic pulmonary diseases, and other chronic undiagnosed diseases were studied. The samples were processed for cytological and microscopic examination by Ziehl-Neelsen staining. Following the decontamination procedure, the aspiration and lymph node tissue samples were inoculated into Middlebrook 7H11 media for up to 8 weeks. The aspirated material was also directly used for molecular detection by triplex-nested polymerase chain reaction (nPCR) assay. Results: A cytological study revealed pyogranulomatous inflammation of the lymph node tissue. Impression smears from lymph node tissues displayed the presence of acid-fast organisms. Out of 83 cases of dogs, 8 were found to be positive for Mycobacterium spp. Among those 8 positive cases, 3 were confirmed to belong to MAC, and 5 belonged to the Mycobacterium tuberculosis complex (MTB complex). Conclusion: MAC and MTB are the underestimated bacteria that could be the causative agents of lymphadenitis in animals.

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References

Alvarez, J; García, IG; Aranaz, A; Bezos, J; Romero, B; De Juan, L and Domínguez, L (2008). Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes. J. Clin. Microbiol., 46: 1246-1251.
Campora, L; Corazza, M; Zullino, C; Ebani, VV and Abramo, F (2011). Mycobacterium avium subspecies hominissuis disseminated infection in a Basset Hound dog. J. Vet. Diagn. Invest., 23: 1083-1087.
Chatterjee, M; Bhattacharya, S; Karak, K and Dastidar, SG (2013). Effects of different methods of decontamination for successful cultivation of Mycobacterium tuberculosis. Indian J. Med. Res., 138: 541-548.
Chen, P; Shi, M; Feng, GD; Liu, JY; Wang, BJ; Shi, XD and Zhao, G (2012). A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid. J. Clin. Microbiol., 50: 1166-1170.
Friend, SCE; Russell, EG; Hartley, WJ and Everist, P (1979). Infection of a dog with Mycobacterium avium serotype II. Vet. Pathol., 16: 381-384.
Gajendra, S; Jha, B; Goel, S; Sahni, T; Sharma, R; Shariq, M and Sachdev, R (2015). Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int. J. Lab. Hematol., 37: 774-782.
Ghielmetti, G and Giger, U (2020). Mycobacterium avium: An emerging pathogen for dog breeds with hereditary immunodeficiencies. Curr. Clin. Microbiol., 7: 67-80. https://doi.org/10.1007/s40588-020-00145-5.
Gopinath, K and Singh, S (2009). Multiplex PCR assay for simultaneous detection and differentiation of Mycobacterium tuberculosis, Mycobacterium avium complexes and other Mycobacterial species directly from clinical specimens. J. Appl. Microbiol., 107: 425-435.
Hackendahl, NC; Mawby, DI; Bemis, DA and Beazley, SL (2004). Putative transmission of Mycobacterium tuberculosis infection from a human to a dog. J. Am. Vet. Med. Assoc., 225: 1573-1577.
Hsiao, PF; Tzen, CY; Chen, HC and Su, HY (2003). Polymerase chain reaction based detection of Mycobacterium tuberculosis in tissues showing granulomatous inflammation without demonstrable acid-fast bacilli. Int. J. Dermatol., 42: 281-286.
Inderlied, CB; Kemper, CA and Bermudez, LE (1993). The Mycobacterium avium complex. Clin. Microbiol. Rev., 6: 266-310.
Kyriakopoulos, AM; Tassios, PT; Matsiota-Bernard, P; Marinis, E; Tsaousidou, S and Legakis, NJ (1997). Characterization to species level of Mycobacterium avium complex strains from human immunodeficiency virus-positive and-negative patients. J. Clin. Microbiol., 35: 3001-3003.
Lam, A; Fostera, D; Martin, P; Spielman, D; Chee, H; Strong, M; Fyfe, J and Malik, R (2012). Treatment of Mycobacterium avium infection in a dog. Aust. Vet. Pract., 42: 234-239.
Laprie, C; Duboy, J; Malik, R and Fyfe, J (2013). Feline cutaneous mycobacteriosis: a review of clinical, pathological and molecular characterization of one case of Mycobacterium microti skin infection and nine cases of feline leprosy syndrome from France and New Caledonia. Vet. Dermatol., 24: 561-569.
Martinho, APV; Franco, MMJ; Ribeiro, MG; Perrotti, IBM; Mangia, SH; Megid, J and Paes, AC (2013). Case Report: Disseminated Mycobacterium tuberculosis infection in a dog. Am. J. Trop. Med. Hyg., 88: 596-600.
Mayskaya, MU; Otten, TF; Ariel, BM; Fedotova, EP; Hunter, RL and Nasyrov, RA (2014). Morphological manifestations of the atypical mycobacteriosis caused by nontuberculous mycobacteria in the HIV infected patients. Ann. Clin. Lab. Sci., 44: 131-133.
Nasr, EA; Marwah, M; Abdel Rahman, M and Shafeek, H (2016). Comparison of modified decontamination methods with culture systems for primary isolation of Mycobacterium bovis from bovine tissues. Benha Vet. Med. J., 30: 59-67.
OIE (2018). Avian tuberculosis. OIE Terrestrial Manual 2018: Office International des Epizooties (OIE), Chapter 3.3.6. Paris, France. PP: 860-868.
Park, H; Kim, C; Park, KH and Chang, CL (2006). Development and evaluation of triplex PCR for direct detection of mycobacteria in respiratory specimens. J. Appl. Microbiol., 100: 161-167.
Ramos, SJ; Woodward, MC; Wakamatsu, N; Bolin, SR and Friedman, ML (2019). Cutaneous manifestation of Mycobacterium avium complex infection in an Australian shepherd dog. Vet. Rec. Case Rep., 7: e000934.
Reddy, VK; Aparna, S; Prasad, CE; Srinivas, A; Triveni, B; Gokhale, S and Moorthy, KK (2008). Mycobacterial culture of fine needle aspirate-A useful tool in diagnosing tuberculous lymphadenitis. Indian J. Med. Microbiol., 26: 259-261.
Sharp, E; Taylor, S and O’Halloran, C (2019). Unusual presentation of canine Mycobacterium avium infection. Vet. Rec., 184: 800.
Shin, SJ; Lee, BS; Koh, WJ; Manning, EJ; Anklam, K; Sreevatsan, S and Collins, MT (2010). Efficient differentiation of Mycobacterium avium complex species and subspecies by use of five-target multiplex PCR. J. Clin. Microbiol., 48: 4057-4062.
Thorel, MF; Huchzermeyer, HF and Michel, AL (2001). Mycobacterium avium and Mycobacterium intracellulare infection in mammals. Rev.Off. Int. Epizoot. 20: 204-218.
Zanini, MS; Moreira, EC; Lopes, MTP; Oliveira, RS; Leao, SC; Fioravanti, RL and Salas, CE (2001). Mycobacterium bovis: polymerase chain reaction identification in bovine lymph node biopsies and genotyping in isolates from Southeast Brazil by spoligotyping and restriction fragment length polymorphism. Mem. Inst. Oswaldo Cruz. 96: 809-813.

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