Molecular detection, genetic diversity, and phylogenetic analysis of foot-and-mouth disease virus (FMDV) type O in Iran during 2015-2016

Document Type : Full paper (Original article)

Authors

1 Ph.D. Student in Microbiology, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Foot and Mouth Disease Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

4 Department of Basic Sciences, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

Abstract

Background: The major challenge of foot-and-mouth disease (FMD) control is attributed to the rapid mutations in the FMDV RNA genome, resulting in continuous antigenic changes of circulating strains. Despite widespread vaccination of livestock populations, the incidence of the FMDV serotype O outbreaks in Iran during 2015-2016 raised concerns about the emergence of new strains. Aims: The aim of this study is the genetic and antigenic evaluation of FMDV type O isolates from different outbreak areas including Alborz, Tehran, Isfahan, Markazi, Zahedan, and Qom provinces. Methods: For this purpose, 71 FMD-infected samples were collected from six provinces of Iran, of which 12 serotype O positive were selected for genetic analysis. Results: All samples were in ME-SA topotypes/OPanAsia2 lineage, and the overall mean of genetic diversities at the 1D gene level was about 5% between the sequences. Blasting 1D gene sequences of isolated viruses showed more than 90% genetic identity with sequences registered from neighboring countries; therefore, it could be concluded that they had a common origin. Six isolates showed the highest genetic diversity (6% to 11%) with the OPanAsia2 vaccine strain (JN676146), which three of them (Qom, Alborz, and Zahedan isolates), had less than 30% antigenic homology with the OPanAsia2 virus (JN676146). Conclusion: Results of this study suggested OPanAsia2 vaccine had no enough coverage with some circulating strains in outbreak areas in Qom, Alborz, and Zahedan provinces, and the necessity of OPanAsia2 replacement with a new vaccine strain in Iran.

Keywords

References

Aiewsakun, P; Pamornchainavakul, N and Inchaisri, C (2020). Early origin and global colonisation of foot-and-mouth disease virus. Sci. Rep., 10: 1-9.
Bagheri Amiri, F; Bahonar, AR; Mostafavi, E; Mansournia, MA; Rasouli, N; Fallah Mehrabadi, MH; Sholepash, MR and Abdollahi, D (2016). Study of the determinants of foot-and-mouth disease in Iran: A unit level case control study. Iranian J. Epidemiol., 12: 62-70.
Brehm, KE; Kumar, N; Thulke, HH and Haas, B (2008). High potency vaccines induce protection against heterologous challenge with foot-and-mouth disease virus. Vaccine. 26: 1681-1687.
Brito, B; Pauszek, SJ; Hartwig, EJ; Smoliga, GR; Vu, LT; Dong, PV; Stenfeldt, C; Rodriguez, LL; King, DP; Knowles, NJ and Bachanek-Bankowska, K (2018). A traditional evolutionary history of foot-and-mouth disease viruses in Southeast Asia challenged by analyses of non-structural protein coding sequences. Sci. Rep., 8: 1-13.
Brito, BP; Rodriguez, LL; Hammond, JM; Pinto, J and Perez, AM (2017). Review of the global distribution of foot-and-mouth disease virus from 2007 to 2014. Transbound. Emerg. Dis., 64: 316-332.
Carrillo, C; Tulman, ER; Delhon, G; Lu, Z; Carreno, A; Vagnozzi, A; Kutish, GF and Rock, DL (2005). Comparative genomics of foot-and-mouth disease virus. J. Virol., 79: 6487-6504.
Di Nardo, A; Ferretti, L; Wadsworth, J; Mioulet, V; Gelman, B; Karniely, S; Scherbakov, A; Ziay, G; Özyörük, F; Parlak, Ü and Tuncer-Göktuna, P (2021). Evolutionary and ecological drivers shape the emergence and extinction of foot-and-mouth disease virus lineages. Mol. Biol. Evol., 38: 4346-4361.
Domingo, E; Escarmı́s, C; Baranowski, E; Ruiz-Jarabo, CM; Carrillo, E; Núñez, JI and Sobrino, F (2003). Evolution of foot-and-mouth disease virus. Virus Res., 91: 47-63.
Firouzi Bandpay, MR; Amighi, M; Lombard, M; Piroird, R and Salehizadeh, M (1984). The situation of foot and mouth disease virus types and subtypes in Iran in 1980-1983. Arch. Razi. Inst., 34: 55-60.
Ilbeigi, K; Bokaie, S; Aghasharif, S; Soares Magalhães, RJ and Rashtibaf, M (2018). Risk factors for recurrence of FMD outbreaks in Iran: a case-control study in a highly endemic area. BMC Vet. Res., 14: 1-7.
Jamal, SM and Belsham, GJ (2013). Foot-and-Mouth disease: Past, present and future. Vet. Res., 44: 1-14.
Kamalidoost, Y; Bokaie, S; Nadalian, M and Mirzaie, K (2017) Determinants of foot-and-mouth disease in industrial dairy farms of Qazvin province: a case-control study. Iran. J. Rumin. Health Res., 2: 31-40.
Kanwal, S; Saeed, A; Munir, M and Arshad, M (2014). Phylogenetics of foot and mouth disease virus in Punjab, Pakistan. Br. J. Virol., 1: 21-28.
Kimura, M (1980). A simple method for estimating evolutionary rates of base substitutions through com-parative studies of nucleotide sequences. J. Mol. Evol., 16: 111-120.
Knowles, NJ; Samuel, AR; Davies, PR; Midgley, RJ and
Valarcher, JF (2005). Pandemic strain of foot-and-mouth disease virus serotype O. Emerg. Infect. Dis., 11: 1887-1893.
Knowles, NJ; Wadsworth, J; Bachanek-Bankowska, K and King, DP (2016). VP1 sequencing protocol for foot and mouth disease virus molecular epidemiology. Rev. Sci. Tech., 35: 741-755.
Mateu, MG; Hernández, J; Martinez, MA; Feigelstock, D; Lea, S; Pérez, JJ; Giralt, E; Stuart, D; Palma, EL and Domingo, E (1994). Antigenic heterogeneity of a foot-and-mouth disease virus serotype in the field is mediated by very limited sequence variation at several antigenic sites. J. Virol., 68: 1407-1417.
Mattion, N; Goris, N; Willems, T; Robiolo, B; Maradei, E; Beascoechea, CP; Perez, A; Smitsaart, E; Fondevila, N; Palma, E and De Clercq, K (2009). Some guidelines for determining foot-and-mouth disease vaccine strain matching by serology. Vaccine. 27: 741-747.
Mohapatra, JK; Subramaniam, S; Pandey, LK; Pawar, SS; De, A; Das, B; Sanyal, A and Pattnaik, B (2011). Phylogenetic structure of serotype A foot-and-mouth disease virus: global diversity and the Indian perspective. J. Gen. Virol., 92: 873-879.
Naqvi, SS; Bostan, N; Fukai, K; Ali, Q; Morioka, K; Nishi, T; Abubakar, M; Ahmed, Z; Sattar, S; Javed, S and Tariq, A (2022). Evolutionary dynamics of foot and mouth disease virus serotype A and its endemic sub-lineage A/ASIA/Iran-05/SIS-13 in Pakistan. Viruses. 14: 1634-1651.
OIE (2008). Manual of diagnostic tests and vaccines for terrestrial animals. Office International des Epizooties, Paris, France. PP: 1092-1106.
OIE (2015). Manual of diagnostic tests and vaccines for terrestrial animals. PP: 1-24.
OIE (2018). Manual of diagnostic tests and vaccines for terrestrial animals. PP: 433-464.
Samuel, AR and Knowles, NJ (2001). Foot-and-Mouth disease type O viruses exhibit genetically and geo-graphically distinct evolutionary lineages (topotypes). J. Gen. Virol., 82: 609-621.
Sheikh, MB; Rashid, PA; Raheem, Z; Marouf, AS and Amin, KM (2021). Molecular characterization and phylogenetic analysis of foot and mouth disease virus isolates in Sulaimani province, Iraq. Vet. Res. Forum. 12: 247-251.
Tamura, K; Stecher, G and Kumar, S (2021). MEGA11: Molecular evolutionary genetics analysis Version 11. Mol. Biol. Evol., 38: 3022-3027.
Zell, R; Delwart, E; Gorbalenya, AE; Hovi, T; King, AMQ; Knowles, NJ; Lindberg, AM; Pallansch, MA; Palmenberg, AC; Reuter, G and Simmonds, P (2017). ICTV virus taxonomy profile: Picornaviridae. J. Gen. Virol., 98: 2421-2422.

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