Molecular characterization of Mycobacterium bovis strains isolated from cattle and humans by spoligotyping and 24-locus MIRU-VNTR, and prevalence of positive IGRA in slaughterhouse workers in Southern Turkey

Document Type : Full paper (Original article)

Authors

1 Department of Microbiology, Ceyhan Veterinary Faculty, Cukurova University, 01330, Adana, Turkey

2 Tuberculosis Region Laboratory, Tropical Disease and Research Center, Cukurova University, 01330, Adana, Turkey

3 Microbiology Laboratory, Adana City Hospital, 01230, Adana, Turkey

4 MSc, Microbiology Laboratory, Adana Veterinary Control Institute, 01250, Adana, Turkey

5 Department of Microbiology, Medicine Faculty, Cukurova University, 01330, Adana, Turkey

6 Department of Pathology, Ceyhan Veterinary Faculty, Cukurova University, 01330, Adana, Turkey

7 Department of Microbiology, Veterinary Faculty, Kastamonu University, 37150, Kastamonu, Turkey

Abstract

Background: Mycobacterium bovis is a zoonotic member of the Mycobacterium tuberculosis complex with a wide range of hosts, mainly cattle. Molecular epidemiological studies should be conducted to determine the transmission route, zoonotic risk factors, and phylogenetic relationships of M. bovis strains. Aims: This study aimed to characterize bovine and human M. bovis isolates by molecular methods. Methods: Molecular characterization and clonal relationship of strains isolated from tissue and organ samples of 76 cattle with positive tuberculin tests were collected from a slaughterhouse, and four M. bovis strains isolated from clinical materials of patients with suspected pulmonary TB isolates were analyzed using 24-locus MIRU-VNTR and spoligotyping methods. QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen) was used to determine the prevalence of latent TB infection among 21 slaughterhouse personnel including 7 veterinarians, 12 butchers, 1 caretaker, and 1 veterinary technician. Results: SB0288/SIT685 type was detected in both cattle and humans by the spoligotyping method. When evaluating MIRU-VNTR, the presence of a 100% compatible pattern between human and bovine isolates was not detected, but some human samples were found to be 91.6% similar to a bovine sample. In addition, 21 slaughterhouse workers were screened with the interferon gamma-released assay (IGRA) and a 23.8% positivity was detected. Conclusion: Clonal similarity was determined between the bovine and human isolates using the MIRU-VNTR and spoligotyping methods and IGRA positivity in the occupational group suggested that M. bovis might be associated with pulmonary tuberculosis in humans.

Keywords

References

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