Intrauterine proteolytic enzymes therapy hastens expulsion of fetal membranes in dystocia affected buffaloes

Document Type : Full paper (Original article)


Dept. of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India



Background: Difficult calving (dystocia) in buffalo cows is a major obstetrical problem which further leads to metritis complex which encompasses retention of fetal membranes (RFM), puerperal metritis, endometritis and pyometra with impaired future fertility. Aims: The current study was aimed to evaluate the effect of administration of intrauterine proteolytic enzymes on the expulsion of fetal membranes and postpartum fertility in dystociac buffaloes. Methods: Proteolytic enzymes consisting of Trypsin (16mg), Chymotrypsin (16mg) and Papain (8mg) dissolved in 500mL normal saline after 1 hour of assisted delivery in dystociac buffaloes along with the conventional therapy. Results: The treated animals (n=15) expelled fetal membranes with in lesser time (p=0.043) as compared to the control group (n=15) with none in treatment group retaining for more than 24h period. A lesser (26.67 vs 73.33%; p=0.027) postpartum uterine infections developed in treated animals than control group. The interval to first postpartum estrus (p=0.067), service period (p=0.554) and open days (p=0.557) were lesser in the treatment group than control group with postpartum anestrus developed lesser (26.67 vs 66.67%; p=0.066) in the animals treated with enzymatic therapy. Systemic illness (neutrophillia) was reduced in the treatment group animals than control on day 20 (64.55±1.14% vs 70.23±0.99%; p=0.001) and 45 (55.05±1.63% vs 64.92±1.45%; p<0.001) postpartum. Conclusion: It was concluded that proteolytic enzymes therapy after assisted delivery in the dystociac buffalo cows could help in early expulsion of fetal membranes, reduced uterine infections with decreased neutrophils count. 


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