Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran

Document Type : Short paper


1 Department of Clinical Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, IranDepartment of Microbiology, Islamic Azad University, Karaj Branch

3 Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran


Background: Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries. Aims: The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz. Methods: CPV-2 was detected using 555 primer pairs in collected samples. Phylogenetic analysis of CPV-2 subtypes was done using sequencing of the partial length of VP2 gene. Results: Twenty-eight CPV-2 were detected using 555 primer pair. The sequences of isolates were deposited in the GenBank database. Phylogenetic analysis revealed that all CPV-2c subtype isolates had very high sequence identity to China and Zambia that form a distinct cluster. Conclusion: In conclusion, this study revealed the emergence of all CPV-2 variants in dogs in Iran. Thus, the continual monitoring of CPV-2 in domestic dogs should be further conducted on a large scale to determine the predominant variants and their distributions in the country and to follow the dynamics of CPV-2 in the Middle East region of Asia.


Main Subjects

Battilani, M; Modugno, F; Mira, F; Purpari, G; Bella, SD; Guercio, A and Balboni, A (2019). Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017: recurrence of the CPV-2b variant. BMC Vet. Res., 15: 393. doi: org/10.1186/s12917-019-2096-1.
Buonavoglia, C; Martella, V; Pratelli, A; Tempesta, M; Cavalli, A; Buonavoglia, D; Bozzo, G; Elia, G; Decaro, N and Carmichael, L (2001). Evidence for evolution of canine parvovirus type 2 in Italy. J. Gen. Virol., 82: 3021-3025.
Calderon, MG; Romanutti, C; Antuono, AD; Keller, L; Mattion, N and Torre, JL (2011). Evolution of canine parvovirus in Argentina between years 2003 and 2010: CPV-2c has become the predominant variant affecting the domestic dog population. Virus Res., 157: 106-110. doi: 10.1016/j.viruses.2011.02.015.
Charoenkul, K; Tangwangvivat, R; Janetanakit, T; Boonyapisitsopa, S; Bunpapong, N; Chaiyawong, S and Amonsin, A (2019). Emergence of canine parvovirus type 2c in domestic dogs and cats from Thailand. Transbound. Emerg. Dis., 66: 1518-1528. doi: org/10.1111/tbed.13177.
Chastant, S and Mila, H (2019). Passive immune transfer in puppies. Anim. Reprod. Sci., 207: 162-170.
Day, MJ; Horzinek, MC; Schultz, RD and Squires, RA (2016). WSAVA guidelines for the vaccination of dogs and cats. J. Small Anim. Pract., 57: E1-E45.
Decaro, N and Buonavoglia, C (2012). Canine parvovirus — A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet. Microbiol., 155: 1-12.
Decaro, D; Buonavoglia, CBVR and Barrs, VR (2020). Canine parvovirus vaccination and immunisation failures: Are we far from disease eradication? Vet. Microbiol., 247: 108760. doi: org/10.1016/j.vetmic.2020.108760.
Decaro, N; Desario, C; Addie, DD; Martella, V; Vieira, MJ; Elia, Zicola, A; Davis, C; Thompson, G; Thiry, E; Truyen, U and Buonavoglia, C (2007). Molecular epidemiology of canine parvovirus, Europe. Emerg. Infect. Dis., 13: 1222-1224.
Fagbohun, OA and Omobowale, TO (2018). Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria. Virusdisease. 29: 411-415. doi: org/10.1007/s13337-018-0475-z.
Figueiredo, J; Miranda, C; Souto, R; Silva, E; Fafetine, J and Thompson, G (2016). Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique. Arch. Microbiol., doi: 10.1007/s00203-016-1320-7.
Firoozjaii, HA; Shoorijeh, SJ; Mohammadi, A and Tamadon, A (2011). Characterization of Iranian isolates of canine parvovirus in fecal samples using polymerase chain reaction assay. Iran. J. Biotechnol., 9: 63-68.
Ford, RB; Larson, LJ; McClure, KD; Schultz, RD and Welborn, LV (2017). 2017 AAHA canine vaccination guidelines. J. Am. Anim. Hosp. Assoc., 53: 243-251.
Hong, C; Decaro, N; Desario, C; Tanner, P; Pardo, MC; Sanchez, S; Buonavoglia, C and Saliki, JT (2007). Occurrence of canine parvovirus type 2c in the United States. J. Vet. Diagn., 19: 535-539.
Kapiya, J; Nalubamba, KS; Kaimoyo, E; Changula, K; Chidumayo, N; Saasa, N; Simuunza, MC; Takada, A; Mweene, AS; Chitanga, S and Simulundu, E (2019). First genetic detection and characterization of canine parvovirus from diarrheic dogs in Zambia. Arch. Virol., 164: 303-307. doi: org/10.1007/s00705-018-4068-3.
Miranda, C and Thompson, G (2016). Canine parvovirus: the worldwide occurrence of antigenic variants. J. Gen. Virol., 97: 2043-2057.
Mosallanejad, B; Vakili, N; Avizeh, R; Seyfiabad Shapouri, MR and Pourmahdi, M (2014). A comparison between PCR and Immunochromatography assay (ICA) in diagnosis of hemorrhagic gastroenteritis caused by canine parvovirus. Arch. Razi Inst., 69: 27-33.
Nikbakht, Gh; Jamshidi, Sh and Mohyedini, Sh (2018). Detection of a new canine parvovirus mutant in Iran. Iran J. Vet. Med., 12: 1-7. doi: 10.22059/ijvm.2018.231479. 1004805.
Novosel, D; Tuboly, T; Balka, G; Szeredi, L; Lojkic, I; Jungic, A and Csagola, A (2019). Evidence of CPV2c introgression into Croatia and novel insights into phylogeny and cell tropism. Sci. Rep., 9: 1-12.
Saei, HD; Javadi, S; Akbari, S; Hadian, N and Zarza, E (2017). Molecular characterization of canine parvovirus (CPV) antigenic variants from healthy and diarrheic dogs in Urmia region, Iran. Iran J. Vet. Med., 11: 9-19.
Voorhees, IEH; Lee, H; Allison, AB; Lopez-Astacio, R; Goodman, LB; Oyesola, OO; Omobowale, O; Fagbohun, O; Dubovi, EJ; Hafenstein, SL; Holmes, EC and Parrish, CR (2020). Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread. J. Virol., 94: e01162-19. doi: org/10.1128/JVI.01162-19.
Yi, L; Tong, M; Cheng, Y; Song, W and Cheng, S (2016). Phylogenetic analysis of canine parvovirus VP2 gene in China. Transbound. Emerg. Dis., 63: e262-e269.
Zhao, Y; Lin, Y; Zeng, X; Lu, C and Hou, J (2013). Genotyping and pathobiologic characterization of canine parvovirus circulating in Nanjing, China. Virol. J., 10: 1-10. doi: 10.1186/1743-422X-10-272.
Zhou, L; Tang, Q; Shi, L; Kong, M; Liang, L; Mao, Q; Bu, B; Yao, L; Zhao, K; Cui, S and Leal, E (2016). Full-length genomic characterization and molecular evolution of canine parvovirus in China. Virus Genes., 52: 411-416. doi: 10.1007/s11262-016-1309-y.
Zhou, P; Zeng, W; Zhang, X and Li, S (2017). The genetic evolution of canine parvovirus - A new perspective. Plos One. 12: e0175035. doi: org/10.1371/journal.pone. 0175035.