Prevalence of avian influenza H5, H7, and H9 viruses in commercial layers in Karachi, Pakistan

Document Type : Short paper

Authors

1 Department of Veterinary Pathology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam, Pakistan

2 Research and Development Section, Sindh Institute of Animal Health, Karachi, Pakistan

Abstract

Background: Avian influenza viruses (AIVs) cause significant harm to the poultry industry due to mortality as well as high morbidity along with the risk of potential zoonotic transmission to humans. Aims: The study aimed to investigate the prevalence of influenza H5, H7, and H9 viruses and their co-infections in layers having respiratory distress such as sneezing, coughing, and tracheal rales. Methods: Totally, 960 tracheal swabs (240 swabs in each season) were collected from 120 poultry flocks, including 10 farms per month and 8 samples per flock, located in Karachi where the outbreaks were reported. The samples were confirmed through antigen ELISA and subtyped by RT-PCR. Results: Antigen ELISA revealed that the prevalence of avian influenza viruses was 26.45%; however, seasonal differences were not significant (P<0.05). RT-PCR subtyping of hemagglutinin (HA) gene revealed the higher prevalence of H9 virus (40.16%) as compared to H7 virus (5.51%) and H5 virus (4.73%). The co-infections comprised H5/H7/H9 (37.0%) and H5/H9 (12.6%). Conclusion: This study shows that AI is endemic in layer farms in Karachi where the H9 subtype is predominant along with co-infections of H5/H7/H9 subtypes.

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References

Ahmed, A; Khan, TA; Kanwal, B; Raza, Y; Akram, M; Rehmani, SF; Nazir, AL and Shahana, UK (2009). Molecular identification of agents causing respiratory infections in chickens from southern region of Pakistan from October 2007 to February 2008. Int. J. Agri. Biol., 11: 325-328.
Capua, I and Alexander, DJ (2007). Avian influenza infections in birds-a moving target. Infl. and Oth. Resp. Vir., 1: 11-18.
Channa, AA; Kalhoro, NH; Nizamani, ZA; Mangi, AH and Soomro, J (2020). Prevalence of Newcastle disease virus and avian influenza virus (H7N3) in poultry at Karachi. RADS J. Biol. Res. Appl. Sci., 11: 9-14.
Kageyama, T; Fujisaki, S; Takashita, E; Xu, H; Yamada, S; Uchida, Y; Neumann, G; Saito, T; Kawaoka, Y and Tashiro, M (2013). Genetic analysis of novel avian A (H7N9) influenza viruses isolated from patients in China, February to April 2013. Euro Surveill., 18: 20453.
Karlsson, EA; Horm, SV; Tok, S; Tum, S; Kalpravidh, W; Claes, F; Osbjer, K and Dussart, P (2019). Avian influenza virus detection, temporality and co-infection in poultry in Cambodian border provinces, 2017-2018. Emerg. Micro. Infec., 8: 637-639.
Muneer, MA; Bahram, AM; Munir, Z; Hussain, I; Muhammad, K; Rabbani, M; Akhtar, S; Aleem, M; Sultan, B; Tariq, MA and Naeem, K (2001). Isolation and characterization of avian influenza (H9N2) virus from
an outbreak at poultry farms in Karachi [Pakistan]. Pak. Vet. J., 21: 87-91.
Naeem, K (1995). An outbreak of avian influenza in poultry in Pakistan. Vet. Rec., 137: 439.
Naeem, K; Siddique, N; Ayaz, M and Jalalee, M (2007). Avian influenza in Pakistan: outbreaks of low- and high-pathogenicity avian influenza in Pakistan during 2003-2006. Avian Dis., 51: 189-193.
Rahman, M; Rabbani, M; Uddin, M; Chakrabartty, A and Her, M (2012). Prevalence of Avian Influenza and Newcastle Disease Viruses in poultry in selected areas of Bangladesh using rapid antigen detection kit. Arch. Clin. Microbiol., 3: 1-8.
Senne, DA; Panigrahy, B; Kawaoka, Y; Pearson, JE; Suss, J; Lipkind, M and Webster, RG (1996). Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis., 40: 425-437.
Shehata, AA; Sedeik, ME; Elbestawy, AR; El-Abideen, MAZ; Ibrahim, HH; Kilany, WH and Ali, A (2019). Co-infections, genetic, and antigenic relatedness of avian influenza H5N8 and H5N1 viruses in domestic and wild birds in Egypt. Poult. Sci., 98: 2371-2379.
Soomro, SA; Soomro, NM; Nizamani, ZA; Kalhoro, NH and Bughio, S (2016). Comparative pathology of experimentally induced low pathogenic avian influenza (H7N3) infection in chicken, ducks and quails. Pak. J. Agr. Agr. Eng. and Vet. Sci., 32: 284-294.
Wing, EAS (2018). Finance Division, Government of Pakistan. Pakistan Economic Survey, 2019.
Wood, GW; McCauley, JW; Bashiruddin, JB and Alexander, DJ (1993). Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of H5 and H7 subtypes. Arch. Virol., 130: 209-217.
Zaman, A; Haleem, A; Rahman, SU; Amin, A; Saeed, A; Ullah, S and Ullah, N (2018). Seroprevalence of avian influenza (H5) in broilers from five districts of Khyber Pakhtunkhwa, Pakistan. Pak. J. Zool., 50: 1687-1691.

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