L-carnitine in a certain concentration increases expression of cell surface marker CD34 and apoptosis in the rat bone marrow CD34+ hematopoietic stem cells

Document Type : Full paper (Original article)

Author

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

10.22099/ijvr.2021.39045.5677

Abstract

Background: Stem cell-based therapy has encouraged as an attractive method in regenerative medicine. Poor survival and maintenance of the cells transferred into the damaged tissue are broadly accepted as serious barriers to enhance the efficacy of the regenerative medicine. For this reason, some antioxidants such as L-carnitine (LC) are used as a favorite strategy to improve the cell survival and retention properties. Aims: This study aims to evaluate the effect of LC on the expression of CD34 marker and its effect on apoptosis and SUZ12 gene expression as well. Methods: The rat bone marrow mono-nuclear cells (rBMNCs) were isolated. Then, CD34+ hematopoietic stem cells (HSCs) were enriched by MACS method. The cells were treated with 0.2 and 0.4 mM LC, and then gene and protein expression level of the CD34 was measured by Real-time PCR and flow cytometry, respectively. The percentage of apoptosis and SUZ12 gene expression were measured using Annexin V/PI method and Real-time PCR, respectively. Results: The results showed that in the experimental group of CD34+ HSCs treated with 0.2 mM LC, gene and protein expression of CD34 increased by 1.7 fold and 0.49%, respectively. At the concentration of 0.4 mM, the early cell apoptosis increased by 25.9% (*P<0.05). Also, in the concentration of 0.2 and 0.4 mM LC, the SUZ12 gene expression increased by 1.10 and 1.75 folds when compared to the control group (*P<0.05 and **P<0.01), respectively. Conclusion: The results of this study could have an effective role in the improvement of CML as a multidirectional therapeutic strategy.

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