Molecular characterization and the full sequencing of chicken infectious anemia virus (CIAV) in Iran

Document Type : Short paper


Department of Poultry Diseases, Faculty of Veterinary Medicine, Azad University, Karaj, Iran



Background: The chicken infectious anemia virus (CIAV) is an important pathogen that causes severe immunosuppression in young chickens. Aims: The study aims to characterize the genotype and full-length sequencing of CIAV strains in Iran. Methods: First the collected thymus tissue samples were investigated by conventional PCR for CIAV detection. In the second phase, one of the CIAV positive samples (UT-Zahraee) was chosen for full genome sequencing. Results: Over the course of 2017, we detected 13 CIAVs isolated from 40 broiler flocks of different provinces of Iran. A comparison of the complete sequences and homologies of the nucleotides revealed that UT-Zahraee had high similarity with American and Egyptian CIAV isolates. Moreover, VP1 sequence analysis showed that UT-Zahraee shared high homology with previously reported Iranian CIAV strains, Chinese, and Egyptian isolates. Conclusion: . This study is the first report of a full genome CIAV strain from Iran. It will be beneficial to understand the epidemiology and molecular characteristics of the CIAV circulating in Iran.


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