Phylogenetic relationship and virulence gene profiles of avian pathogenic and uropathogenic Escherichia coli isolated from avian colibacillosis and human urinary tract infections (UTIs)

Document Type : Full paper (Original article)

Author

Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

10.22099/ijvr.2021.40081.5810

Abstract

Background: There is some evidence representing the possible relation between avian pathogenic E. coli (APEC) and other extraintestinal pathogenic E. coli (ExPEC) strains such as human uropathogenic isolates. Aims: ‏‏The present study was conducted to evaluate virulence and phylogenetic associations between a total of 70 APEC and UPEC isolates (35 APEC and 35 UPEC isoaltes) obtained in the north of Iran as one of the particular centers of the poultry industry in the country. Methods: Polymerase chain reaction (PCR) and random amplified polymorphism DNA (RAPD) analysis were done respectively using specific primers and data was analyzed using capable software. Results: the most prevalent gene was fliC (70.6%) followed by fimH (67.1%) but APEC and UPEC isolates showed inordinate and obvious differences in the presence of some virulence genes such as fliC, hlyD, and sfa1 and predominant phylogenetic groups in DNA fingerprinting methods. Conclusion: Results of the study showed that although there are obvious differences between isolates of APEC and UPEC in terms of phylogenetics and pattern of virulence gene, APEC isolates despite having virulence genes such as papC, ibeA and iss can have a high potential for incidence of disease in humans and generate dangerous outbreaks in communities with low levels of health and to poultry.

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