The anti-proliferative and apoptotic effects of curcumin on feline mammary gland tumor cells in vitro

Document Type : Full paper (Original article)

Author

Department of Medical Biology, Faculty of Medicine, Sakarya University, Sakarya, Turkey

10.22099/ijvr.2021.40452.5866

Abstract

Backround: Feline mammary gland tumors (FMGTs) are the third most diagnosed tumors in cats. FMGTs have aggressive biological behavior and poor response to both surgical and medical treatments and new therapeutic approaches are needed to improve. Curcumin (CUR) is a polyphenol component and exhibits the anti-cancer effects and induces apoptosis through different mechanisms especially in human breast cancer. However, there is no study investigating the effects of CUR on FMGTs. Aims: Therefore, the aim of this study was to determine the anti-proliferative and apoptotic effects of CUR on primary cell lines from FMGT tissue samples of two cases which were classified as carcinoma-simple, tubular type (Grade III). Methods: The cytotoxic effect of CUR was determined by WST-1 assay. Annexin V, cell cycle and AO analysis were performed to determine the apoptotic effect of CUR. Results: Our results showed that CUR had an anti-proliferative and apoptotic effect through induction of apoptotic cells and G0/G1 cell cycle arrest on FMGT cells. Conclusion: Therefore, this is the first study that show the effects of CUR on FMGTs. However, further molecular studies are required to compare the effects of CUR on different histopathological phenotypes and to determine the further molecular mechanisms including the potential apoptotic and cellular pathways affected by CUR.

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