Effects of citral on serum antioxidant status and liver gene expression of paraoxonase 1 and nitric oxide synthase in a rat model of streptozotocin-induced diabetes mellitus

Document Type : Full paper (Original article)


Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran



Background: Citral (C10H16O) is a main ingredient of lemongrass oil and can reduce the side effects of oxidative stress. Diabetes caused by insulin deficiency induces oxidative stress in the liver. Aimes: The aims of this study were to investigate the ameliorative effects of citral on selected oxidative parameters and the gene expression of Paraoxonase 1 (PON1) and endothelial nitric oxide synthase (eNOS) in a rat model of streptozotocin (STZ)-induced diabetes mellitus. Methods: Forty rats were divided into four groups at random: Control (C), Control Citral (CC) and two STZ-induced diabetic groups (Diabetic (D) and Citral Diabetic (CD)). After diabetes confirmation (day 7), gavage treatment with citral (300 mg/kg body weight) was started in the CD and CC groups and continued for two weeks. Results: On day 21 of the study, following treatment with citral for 14 days, the serum levels of total antioxidant capacity (TAC), and PON1 in the CD group were significantly increased compared to those in the D group (p<0.05). While, treatment with citral, caused a significant decrease in the MDA, and eNOS in the CD group compared to those of D group (P<0.001). The expression rate of liver PON1 gene was considerably upregulated in CD group compared to that in the D group (p<0.001); while the opposite was observed for eNOS gene expression. However, there was no significant difference between the CC and C groups in terms of the all examined parameters (p>0.05).  Conclusion: This study showed positive effects of citral on serum antioxidant status and liver gene expression of PON1 and eNOS in diabetic rat.


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