Ovine visceral organs as reservoir candidate for Brucella abortus in Iran

Document Type : Short paper

Authors

1 Graduated from Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran

3 Brucellosis Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

10.22099/ijvr.2021.38239.5567

Abstract

Background: Brucellosis is an important zoonotic disease in Iran. There are several reservoirs for this disease in nature. There is little information in this regard in Iran. Aims: The present study investigated the prevalence and diversity of Brucella species in ovine reticuloendothelial organs including liver, spleen, intestine (mesenteric lymph nodes), and lung. This help to address the ability of the reservoir species in disease transmission to other animals through these organs. Methods: A total of 200 ovine visceral organs including 44 intestines, 51 lungs, 52 spleens, and 52 livers were collected. The presence of different Brucella species was studied using the polymerase chain reaction (PCR). Results: The results demonstrated the prevalence of Brucella species in 56 (28.4%) samples. In addition, liver and intestine were the most and the least infected organs, respectively. In general, 48 out of 56 positive samples were infected with Brucella abortus, while 10 samples were infected with Brucella melitensis. Conclusion: Eventually, the results approved the possible presence of B. abortus among domestic animals, especially sheep and cattle, and highlighted the role of sheep as reservoir hosts for its potential transfer to cattle.

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Bagheri, H; Tapak, L; Karami, M; Hosseinkhani, Z; Najari, H; Karimi, S and Cheragh, Z (2020). Forecasting the monthly incidence rate of brucellosis in west of Iran using time series and data mining from 2010 to 2019. PLoS One. 15: e0232910.
Bercovich, Z (1998). Maintenance of Brucella abortus-free herds: a review with emphasis on the epidemiology and the problems in diagnosing brucellosis in areas of low prevalence. Vet. Q., 20: 81-88.
De Figueiredo, P; Ficht, TA; Rice-Ficht, A; Rossetti, CA and Adams, LG (2015). Pathogenesis and immunobiology of brucellosis: review of Brucella--host interactions. Am. J. Pathol., 185: 1505-1517.
Doganay, M and Aygen, B (2003). Human brucellosis: an overview. Int. J. Infect. Dis., 7: 173-182.
El-Nesr, KA; Mahdy, E; Shalaby, A and Deeb, S (2007). Pathological and immunohistochemical studies on Brucella melitensis in cows. Vet. Med. J. Giz. [ZDB], 55: 275.
Ewalt, DR and Bricker, BJ (2000). Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. J. Clin. Microbiol., 38: 3085-3086.
Fatma, HMA and Mahdey, EA (2010). Incidence of Brucella species in slaughtered food animals and its edible offal at Beni-suef, Egypt. Glob. Vet., 5: 248-254.
Foster, G; Osterman, BS; Godfroid, J; Jacques, I and Cloeckaert, A (2007). Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int. J. Syst. Evol. Microbiol., 57: 2688-2693.
Franco, MP; Mulder, M; Gilman, RH and Smits, HL (2007). Human brucellosis. Lancet Infect. Dis., 7: 775-786.
Gwida, M; Al Dahouk, S; Melzer, F; Rösler, U; Neubauer, H and Tomaso, H (2010). Brucellosis--regionally emerging zoonotic disease? Croat Med. J., 51: 289-295.
Kassiri, H; Amani, H and Lotfi, M (2013). Epidemiological, laboratory, diagnostic and public health aspects of human brucellosis in western Iran. Asian Pac. J. Trop. Biomed., 3: 589-594.
Koriem, AM; AL-Habaty, SH; Makar, NA and Abd-El-kader, HA (2013). Seroprevalence of brucellosis in slaughtered animals at Assiut governorate. Assiut. Vet. Med. J., 59: 72-76.
Malik, GM (1997). A clinical study of brucellosis in adults in the Asir region of southern Saudi Arabia. Am. J. Trop. Med. Hyg., 56: 375-377.
Ocholi, RA; Kwaga, JKP; Ajogi, I and Bale, JOO (2005). Abortion due to Brucella abortus in sheep in Nigeria. Rev. Sci. Tech. Off. Int. Epiz., 24: 973-979.
Okoh, AEJ (1980). Abortion in sheep near Kano, Nigeria. Trop. Anim. Health Prod., 12: 11-14.
Pappas, G; Papadimitriou, P; Akritidis, N; Christou, L and
Tsianos, EV
(2006). The new global map of human brucellosis. Lancet Infect. Dis., 6: 91-99.
Sadler, WW (1960). Present evidence on the role of meat in the epidemiology of human brucellosis. Am. J. Public Health Nations Health. 50: 504-514.
Scholz, HC; Hubalek, Z; Sedláček, I; Vergnaud, G; Tomaso, H; Al Dahouk, S; Melzer, F; Kämpfer, P; Neubauer, H; Cloeckaert, A; Maquart, M; Zygmunt, MS; Whatmore, AM; Falsen, E; Bahn, P; Göllner, C; Pfeffer, M; Huber, B; Busse, AJ and Nöckler, K (2008). Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int. J. Syst. Evol. Microbiol., 58: 375-382.
Scholz, HC; Nöckler, K; Göllner, C; Bahn, P; Vergnaud, G; Tomaso, H; Al Dahouk, S; kKämpfer, P; Clockaert, A; Maquart, M; Zygmunt, MS; Whatmore, AM; Pfeffer, M; Huber, B; Busse, HJ and De, BK (2010). Brucella inopinata sp. nov., isolated from a breast implant infection. Int. J. Syst. Evol. Microbiol., 60: 801-808.
Seria, W; Tadese, YD and Shumi, E (2020). A review on brucellosis in small ruminants. Am. J. Zool., 3: 17-25.
Silva, T; Costa, EA; Paixão, TA; Tsolis, RM and Santos, RL (2011). Laboratory animal models for brucellosis research. J. Biomed. Biotechnol., 2011: 1-9.
Sofian, M; Aghakhani, A; Velayati, AA; Banifazl, M; Eslamifar, A and Rmezani, A (2008). Risk factors for human brucellosis in Iran: a case--control study. Int. J. Infect. Dis., 12: 157-161.
Zheludkov, MM and Tsirelson, LE (2010). Reservoirs of Brucella infection in nature. Biol. Bull., 37: 709-715.
Zowghi, E and Ebadi, A (1985). Naturally occurring Brucella melitensis infection in cattle in Iran. Rev. Sci. Tech., 4: 811-814.
Zowghi, E and Ebadi, A (1989). Abortion due to Brucella abortus in sheep in Iran. Arch. Razi Inst., 40: 107-110.