Molecular detection of fowl adenovirus 7 from slaughtered broiler chickens in Iran: the first report

Document Type : Short paper

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran (previous address); Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Avian Diseases Research and Diagnostics, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

4 Peigir Company, Gorgan, Iran

5 Ph.D. Student in Virology, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

6 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Background: Fowl adenoviruses (FAdVs) are responsible for a variety of clinical symptoms, with an increasing significance in the poultry industry throughout the world. Typical diseases caused by FAdVs include inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), gizzard erosion (GE), respiratory disease, and hemorrhage in muscles and organs. Aims: During 2020, broiler chickens from the north of Iran showed ecchymotic and petechial hemorrhages in thigh and breast muscles at the slaughterhouse. Hemorrhages were observed in 10% to 60% (with an average of 20-30%) of chicks per flock. To find out the etiology of these lesions, the present study was conducted. Methods: Different environmental factors were investigated, and FAdV, infectious bursal disease virus (IBDV), and chicken infectious anemia virus (CIAV) were detected using molecular assays. Results: Among the viruses tested, FAdV was detected by polymerase chain reaction (PCR), and sequence analysis clustered the virus into species E, serotype 7. Conclusion: This is the first report on FAdV-7 existence among poultry in Iran. Effective screening of the chicks at slaughtering age should be performed from the whole country.

Keywords

Main Subjects

References

Chen, L; Yin, L; Zhou, Q; Peng, P; Du, Y; Liu, L; Zhang, Y; Xue, C and Cao, Y (2019). Epidemiological investigation of fowl adenovirus infections in poultry in China during 2015-2018. BMC Vet. Res., 15: 271.
Erny, K; Barr, D and Fahey, K (1991). Molecular characterization of highly virulent fowl adenoviruses associated with outbreaks of inclusion body hepatitis. Avian Pathol., 20: 597-606.
Hiremath, C; Jhala, M; Bhanderi, B and Joshi, CG (2013). Cloning and sequence analysis of VP1, VP2 and VP3 genes of Indian chicken anemia virus. Iran. J. Vet. Res., 14: 354-357.
Hosseini, H; Langeroudi, AG; FallahMehrabadi, MH; Kafi, ZZ; Dizaji, RE; Ghafouri, SA; Hamadan, AM; Aghaiyan, L and Hajizamani, N (2019). The fowl adenovirus (Fadv-11) outbreak in Iranian broiler chicken farms: The first full genome characterization and phylogenetic analysis. Comp. Immunol. Microbiol. Infect. Dis., 70: 101365.
Hosseini, H and Morshed, R (2012). Molecular identification of fowl adenovirus associated with inclusion body hepatitis in Iran. Iran. J. Virol., 6: 7-12.
Kaján, GL; Affranio, I; Bistyák, AT; Kecskeméti, S and Benkő, M (2019). An emerging new fowl adenovirus genotype. Heliyon. 5: e01732.
Kaján, GL; Kecskeméti, S; Harrach, B and Benkő, M (2013). Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity. Vet. Microbiol., 167: 357-363.
Kefford, B; Borland, R; Slattery, J and Grix, D (1980). Serological identification of avian adenoviruses isolated from cases of inclusion body hepatitis in Victoria, Australia. Avian Dis., 24: 998-1006.
Khodakaram-Tafti, A; Asasi, K and Namazi, F (2016). Clinicopathological characteristics of acute inclusion body
hepatitis outbreak in broiler chickens in Iran. Bulg. J. Vet. Med., 19: 163-168.
Mittal, D; Jindal, N; Tiwari, AK and Khokhar, RS (2014). Characterization of fowl adenoviruses associated with hydropericardium syndrome and inclusion body hepatitis in broiler chickens. Virus Dis., 25: 114-119.
Morshed, R; Hosseini, H; Langeroudi, AG; Fard, MHB and Charkhkar, S (2017). Fowl adenoviruses D and E cause inclusion body hepatitis outbreaks in broiler and broiler breeder pullet flocks. Avian Dis., 61: 205-210.
Nateghi, E; Razmyar, J and Bassami, MR (2014). Molecular characterization of avian adenoviruses in Iranian broiler flocks. Iran. J. Vet. Res., 15: 164-167.
Rahimi, M and Haghighi, ZMS (2015). Adenovirus-like inclusion body hepatitis in a flock of broiler chickens in Kermanshah province, Iran. Vet. Res. Forum., 6: 95-98.
Razmyar, J and Peighambari, SM (2009). Isolation and characterization of a very virulent infectious bursal disease virus from turkey. Acta Virol., 53: 271-276.
Ren, G; Wang, H; Huang, M; Yan, Y; Liu, F and Chen, R (2019). Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens. Virus Genes., 55: 619-629.
Sapats, SI and Ignjatovic, J (2002). Restriction fragment length polymorphism analysis of the VP2 gene of Australian strains of infectious bursal disease virus. Avian Pathol., 31: 559-566.
Singh, A; Oberoi, M; Grewal, G; Hafez, H and Hess, M (2002). The use of PCR combined with restriction enzyme analysis to characterize fowl adenovirus field isolates from northern India. Vet. Res. Commun., 26: 577-585.
Steer, PA; Kirkpatrick, NC; O’Rourke, D and Noormohammadi, AH (2009). Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region. J. Clin. Microbiol., 47: 311-321.
Tabib Ghafari, P; Boroomand, Z; Rezaie, A; Mayahi, M and Eftekharian, S (2018). Detection and identification of avian adenovirus in broiler chickens suspected of inclusion body hepatitis in Khuzestan, Iran during 2015-2016. Iran. J. Vet. Res., 9: 41-45.

Files

Share

How to cite

Statistics