Three dimensional modeling and quantitative analysis of long bone parameters of rabbit using micro-computed tomography

Document Type : Short paper

Authors

1 Department of Anatomy, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey

2 Department of Basic Sciences, Faculty of Dentistry, Cankiri Karatekin University, Cankiri, Turkey

3 MSc Student in Anatomy, Department of Anatomy, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey

4 Department of Surgery, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, Turkey

5 Department of Biostatistics, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey

6 Department of Nuclear Medicine, Faculty of Medicine, Ankara University, Ankara, Turkey

Abstract

Background: Micro-computed tomography (µCT), a modern imaging technique, provides detailed information on the bone morphology of small animal models. Aims: The objectives are 1) to produce three dimensional (3D) models from µCT images of femoral and tibial bones of New Zealand rabbits, and 2) to estimate and compare morphometric and volumetric results among genders as well as left and right sides. Methods: A total of twenty adult New Zealand rabbits (10 females, 10 males, aged 12-18 weeks, weight= 2.5-3 kg) were used for this study. ‏‏Three dimensional reconstructed models of the femoral and tibial bones of rabbits were created from cross-sectional images of µCT using the 3D Slicer program. Anatomical structures were determined on these 3D bone models. Afterward, morphometric parameters such as length, thickness, and width of various parts of the bones were calculated with volume and volume ratio values of cortical bone, trabecular bone, and medullary cavity. Results: The gender*laterality interaction term was found statistically significant in measurements of femoral diaphysis diameter (FDD), internal femoral diaphysis diameter (IFDD), femoral head diameter (FHD), tibial diaphysis diameter (TDD), tibial distal width (TDH), and tibial proximal width (TPW) (p <0.001). The gender*laterality interaction term was not significant in volume and volume fraction values of cortical bone, trabecular bone, and medullary cavity (P>0.05). Conclusion: It is thought that the study will contribute to the orthopedic experimental studies of rabbits for femoral and tibial bones and will bring a modern perspective to the field of veterinary anatomy.

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References

Ajayi, IE; Shawulu, JC; Zachariya, TS; Ahmed, S and Adah, BM (2012). Osteomorphometry of the bones of the thigh, crus and foot in the New Zealand white rabbit (Oryctolagus cuniculus). Ital. J. Anat. Embryol., 117: 125-134.
Araujo, FAP; Sesoko, NF; Rahal, SC; Teixeira, CR; Müller, TR and Machado, MRF (2013). Bone morphology of the hind limbs in two caviomorph rodents. Anat. Histol. Embryol., 42: 114-123.
Bagi, CM; Berryman, E and Moalli, MR (2011). Comparative bone anatomy of commonly used laboratory animals: Implications for drug discovery. Comp. Med., 61:
76-85.
Bouxsein, ML; Boyd, SK; Christiansen, BA; Guldberg, RE; Jepsen, KJ and Müller, R (2010). Guidelines for assessment of bone microstructure in rodents using micro-computed tomography. J. Bone Miner Res., 25: 1468-1486.
Brewer, NR (2006). Biology of the rabbit. J. Am. Assoc. Lab. Anim. Sci., 45: 8-24.
Estai, M and Bunt, S (2016). Best teaching practices in anatomy education: A critical review. Ann. Anat., 208: 151-157.
Fedorov, A; Beichel, R; Kalpathy-Cramer, J; Finet, J; Fillion-Robin, JC; Pujol, S; Bauer, C; Jennings, D; Fennessy, F; Sonka, M; Buatti, J; Aylward, S; Miller, JV; Pieper, S and Kikinis, R (2012). 3D slicer as an image computing platform for the quantitative imaging network. Magn. Reson. Imagin., 30: 1323-1341.
Hoechel, S; Schulz, G and Gerbl, MM (2015). Insight into the 3D-trabecular architecture of the human patella. Ann. Anat., 200: 98-104.
Jiang, Y; Zhao, J; White, DL and Genant, HK (2000). Micro CT and Micro MR imaging of 3D architecture of animal skeleton. J. Musculoskel. Neuron. Interact., 1: 45-51.
Kim, M; Huh, KH; Yi, WJ; Heo, MS; Lee, SS and Choi, SC (2012). Evaluation of accuracy of 3D reconstruction images using multi-detector CT and cone-beam CT. Imaging Sci. Dent., 42: 25-33.
Kubikova, T; Bartos, M; Juhas, S; Suchy, T; Sauerova, P; Kalbacova, MH and Tonar, Z (2018). Comparison of ground sections, paraffin sections and micro-CT imaging of bone from the epiphysis of the porcine femur for morphometric evaluation. Ann. Anat., 220: 85-96.
Pazvant, G and Kahvecioglu, KO (2009). Studies on homotypic variation of forelimb and hindlimb long bones of rabbits. J. Fac. Vet. Med. Istanbul. Univ., 35: 23-29.
Pazzaglia, UE; Zarattini, G; Giacomini, D; Rodella, L; Menti, AM and Feltrin, G (2010). Morphometric analysis of the canal system of cortical bone: an experimental study in the rabbit femur carried out with standard histology and micro-CT. Anat. Histol. Embryol., 39: 17-26.
Savio, G; Baroni, T; Concheri, G; Baroni, E; Meneghello, R; Longo, F and Isola, M (2016). Computation of femoral canine morphometric parameters in three-dimensional geometrical models. Vet. Surg., 45: 987-995.
Stull, KE; Tise, ML; Ali, Z and Fowler, DR (2014). Accuracy and reliability of measurements obtained from computed tomography 3D volume rendered images. Forensic Sci. Int., 238: 133-140.
von den Driesch, A (1976). A guide to the measurement of animal bones from Archaeological sites. 1st Edn., Cambridge, Massachusetts, Harvard University. PP: 84-87.
Wang, HH; Wang, YXJ; Sheng, H; Zhang, G; Qin, L; Ahuja, AT and Teng, LS (2009). Fossa trochanterica of the proximal femur in rabbits: an anatomic structure for potential misinterpretation on magnetic resonance images. Acta Radiol., 50: 212-216.
Zotti, A; Banzato, T and Cozzi, B (2009). Cross-sectional anatomy of the rabbit neck and trunk: Comparison of computed tomography and cadaver anatomy. Res. Vet. Sci., 87: 171-176.

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