Document Type : Full paper (Original article)
Author
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Box: 61355-145, Ahvaz, Iran
10.22099/ijvr.2021.36953.5392
Abstract
Background: The study was performed to detect enterotoxigenic genes of A and B in coagulase-positive and negative staphylococci to evaluate of biotypes and antibiotic resistance of isolated S. aureus from different meat samples. Methods: A total of 160 meat swab samples were collected from lamb, water buffalo, cattle and chicken carcasses. Presumptive colonies on Baird Parker Agar were subjected to biochemical identification, including Gram staining, catalase, oxidase and coagulase activity. Then relevant colonies separately were subjected to the PCR assay for identification of Staphylococcus genus, enterotoxigenic genes (sea and seb) and the thermonuclease gene (Nuc) specific for the S. aureus. The antibiotic susceptibility testing was also carried out using five antibiotics. Results: Totally 150 Staphylococcus spp. were isolated from the samples. Of these, 135 (90%) isolates harboring sea gene, meanwhile no one contained seb gene. Twenty-five S. aureus confirmed by PCR, from which 15 isolates (60%) belonged to host specific (HS), 7 isolates (28%) belonged to none host specific (NHS) biotypes and 3 isolates (12%) cannot be biotyped. Overall, 68%, 56%, 16%, 12% and 8% of isolates were resistant to penicillin, trimethoprim, gentamicin, oxacillin and erythromycin, respectively. Conclusion: The meats supplied in this area were contaminated with enterotoxigenic and antibiotic-resistance staphylococci, which can threaten the health of the consumers. The study showed that not only coagulase positive staphylococci contain enterotoxin A gene, coagulase negative isolates were also possess this gene, especially in buffalo meat, and thus they are considered potential hazards in different meats.
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