Molecular characterization and phylogenetic analysis of a VIIl sub-genotype of Avian orthoavulavirus 1 isolated from Eurasian magpie (Pica pica)

Document Type : Short paper

Author

Department of pathobiology, Faculty of veterinary medicine, Amol University of Special of Modern Technologies, Amol, Iran

10.22099/ijvr.2021.37584.5470

Abstract

Background: Newcastle disease has been categorized as a highly contagious viral disease, remaining as a constant threat to both wild bird and commercial chicken. Methods: In this study, we recovered and characterized avian orthoavulavirus 1 (AOaV-1) strain, designed as EM1, from the Eurasian magpie (Pica pica). The nucleotide and amino acid sequence of the fusion protein (F protein) of EM1 were determined and its phylogenetic relationship was investigated with well-characterized AOaV-1 genotypes, which originated from wild bird species and chicken around the world. Results: Phylogenetic analyses and deduced amino acid sequences the F gene revealed that the EM1 virus belongs to VIIl sub-genotype viruses with the characteristic multibasic amino acid sequences associated with the velogenic motif as 112RRQKRF117 at the cleavage site of its precursor fusion protein. EM1 shared high level of nucleotide and amino acid sequences similarity of F protein with viruses in this sub-genotype. Furthermore, the evolutionary difference between studied virus and viruses belong the VIIl sub-genotype indicated that a close relatedness and the possibility of a common origin. Conclusion: These results suggest the continued circulation of virulent AOaV-1 of sub-genotype VIIl in Iran and disseminate virus among wild and domestic bird species that can cause bidirectional spillover infection. Therefore, further epidemiological studies can be beneficial in the assessment of the evolution of AOaV-1 in its hosts and will help us to be well-equipped in facing the emergence of new sub-genotype of this virus.

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