Changes of acute-phase proteins during different phases of the estrous cycle in Ovsynch-synchronized Holstein cows

Document Type : Short paper

Authors

1 Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Graduated from Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

10.22099/ijvr.2020.36856.5377

Abstract

Background: Acute-phase proteins (APPs) may be increased due to different stresses during estrus phase in farm animals. Aims: Determining changes of APPs at different phases of non-synchronized estrous cycle group (NSEG), and Ovsynch-synchronized estrous cycle group (SEG) in Holstein cows. Methods: Twelve non-pregnant clinically and paraclinicaly healthy Holstein cows with a body condition score (BCS) of 2.75 and 70 days in milk were chosen. Two groups including NSEG and SEG were performed. Blood sampling was carried out from NSEG at the time of diestrus, proestrus, and estrus. In SEG, blood sampling was performed on day 7 (at the time of prostaglandin injection, equivalent diestrus), day 9 (at the time of gonadotropin-releasing hormone (GnRH) injection, equivalent proestrus), and day 10 (at the time of insemination, equivalent estrus) of synchronization protocol. Concentrations of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), and fibrinogen (Fib) were measured. Results: Concentration of Hp at estrus phase was significantly higher compared with diestrus (P=0.001) and proestrus (P=0.019) in NSEG. Moreover, Hp level in the NSEG was significantly higher than SEG at estrus phase (P=0.002). Concentrations of SAA, Cp, and Fib had no significant differences during various phases of estrous cycle in each group or between equivalent phases of both groups. Conclusion: It seems that unlike SAA, Fib, and Cp, concentrations of Hp may be affected by different phases of estrous cycle. Although APPs are not specific indicators, their changes besides other clinical and paraclinical indices may be helpful for more accurate heat detection in dairy cows.

Keywords

Main Subjects


Al-Gubory, KH; Fowler, PA and Garrel, C (2010). The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. Int. J. Biochem. Cell. Biol., 42: 1634-1650.
Ceciliani, F; Ceron, J; Eckersall, P and Sauerwein, H (2012). Acute phase proteins in ruminants. J. Proteomics. 75: 4207-4231.
Constable, PD; Hinchcliff, KW; Done, SH and Grünberg, W (2017). Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs and goats. 11th Edn., London, UK, Elsevier. PP: 2045-2100.
Cooke, R; Arthington, J; Araujo, D and Lamb, G (2009). Effects of acclimation to human interaction on performance, temperament, physiological responses, and pregnancy rates of Brahman-crossbred cows. J. Anim. Sci., 87: 4125-4132.
El-Deeb, WM and El-Bahr, SM (2017). Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokines. Vet. Arhiv., 87: 431-440.
Fischer, C; Drillich, M; Odau, S; Heuwieser, W; Einspanier, R and Gabler, C (2010). Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of subclinical or clinical endometritis. Reprod. Fert. Develop., 22: 818-829.
Kim, MH; Yang, JY; Upadhaya, SD; Lee, HJ; Yun, CH and Ha, JK (2011). The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. J. Vet. Sci., 12: 151-157.
Krakowski, L and Zdzisinska, B (2007). Selected cytokines and acute phase proteins in heifers during the ovarian cycle course and in different pregnancy periods. Bull. Vet. Inst. Pulawy. 51: 31-36.
Kuru, M; Merhan, O; Kaya, S; Oral, H and Kukurt, A (2015). The effect of short term progesterone-releasing intravaginal device treatment on acute inflammation markers for Holstein heifers. Rev. Med. Vet., 166: 336-340.
Lavery, K; Way, A and Killian, G (2003). Identification and immunohistochemical localization of a haptoglobin-like protein in the tissues and fluids of the bovine (Bos taurus) ovary and oviduct. Reproduction. 125: 837-846.
Murata, H; Shimada, N and Yoshioka, M (2004). Current research on acute phase proteins in veterinary diagnosis: an overview. Vet. J., 168: 28-40.
Nazifi, S; Esmailnezhad, Z; Haghkhah, M; Ghadirian, S and Mirzaei, A (2012). Acute phase response in lame cattle with interdigital dermatitis. World J. Microbiol. Biotechnol., 28: 1791-1796.
Nazifi, S; Rezakhani, A; Koohimoghadam, M; Ansari-Lari, M and Esmailnezhad, Z (2008). Evaluation of serum haptoglobin in clinically healthy cattle and cattle with inflammatory diseases in Shiraz, a tropical area in southern Iran. Bulg. J. Vet. Med., 11: 95-101.
Noakes, DE; Parkinson, TJ and England, GC (2019). Veterinary reproduction and obstetrics. 10th Edn., London, UK, Elsevier. PP: 13-23.
Pursley, J; Mee, M and Wiltbank, M (1995). Synchronization of ovulation in dairy cows using PGF2α and GnRH. Theriogenology. 44: 915-923.
Sciorsci, RL; Galgano, M; Mutinati, M and Rizzo, A (2020). Oxidative state in the estrus cycle of the buffaloes: a preliminary study. Trop. Anim. Health Prod., 52: 1331-1334.
Sunderman, FW and Nomoto, S (1970). Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin. Chem., 16: 903-910.
Thrall, MA (2012). Veterinary hematology and clinical chemistry. 2nd Edn., Philadelphia, USA, Lippincott Williams and Wilkins. PP: 401-415.
Trevisi, E; Amadori, M; Bakudila, A and Bertoni, G (2009). Metabolic changes in dairy cows induced by oral, low-dose interferon-alpha treatment. J. Anim. Sci., 87: 3020-3029.
Ulutas, PA; Musal, B; Kiral, F and Bildik, A (2009). Acute phase protein levels in pregnancy and oestrus cycle in bitches. Res. Vet. Sci., 86: 373-376.