Clinical, bacteriological and histopathological aspects of first-time pyoderma in a population of Iranian domestic dogs: a retrospective study

Document Type : Short paper

Authors

1 Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Background: Staphylococci are the most common cause of pyoderma in dogs. Aims: The purpose of the present study was to investigate clinical, bacteriological and histopathological aspects of bacterial skin infections in a population of Iranian domestic dogs with first-time pyoderma. Methods: The study animals were 61 clinical cases of Iranian domestic dogs with first-time pyoderma. The diagnosis of pyoderma was based on the history, the presence of variable gross cutaneous lesions, positive findings on microscopic examination of surface cytology and histopathological findings. Results: Detection of pyoderma amongst adult dogs was significantly higher than puppies (P=0.001). Large breed dogs were presented more frequently for pyoderma in comparison to small breeds (P=0.002). Bacterial species were recovered from 43 of the 61 (70.49%) studied animals. No isolates were recovered from 18 studied dogs. The most frequently recovered bacterial genus was Staphylococcus (32/43 isolates, 74.41%) including: S. epidermidis (22/43 isolates, 51.16%), S. aureus (7/43 isolates, 16.27%), and S. pseudintermedius (3/43 isolates, 6.97%). Staphylococci species resistance was most commonly seen against amoxicillin (94.11%), penicillin (83.35%), and ampicillin (76.47%). Resistant to cephalexin and cefoxitin was 5.88% and 2.94%, respectively. A total of 27 of the staphylococci isolated (84.37%) were resistant to at least one antimicrobial agent and 19 isolates (59.37%) were resistant to three or more antimicrobial drugs. Conclusion: A better understanding of this microbial population is critical for clarification of the pathophysiology of bacterial skin diseases.

Keywords

References

CLSI (2013). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals approved standard. VET08, 4th Edn., CLSI document Wayne, PA, Clinical and Laboratory Standards Institute.
Devriese, LA; Vancanneyt, M; Baele, M; Vaneechoutte, M; DeGraef, E; Snauwaert, C; Cleenwerck, I; Dawyndt, P; Swings, J; Decostere, A and Haesebrouck, F (2005). Staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals. Int. J. Syst. Evol. Microbiol., 55: 1569-1573.
Fazakerley, J; Nuttall, T; Sales, D; Schmidt, V; Carter, SD; Hart, CA and McEwan, NA (2009). Staphylococcal colonization of mucosal and lesion skin sites in atopic and healthy dogs. Vet. Dermatol., 20: 179-184.
Futagawa-Saito, K; Suzuki, M; Ohsawa, M; Ohshima, S; Sakurai, N; Ba-Thein, W and Fukuyasu, T (2004). Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons. J. Appl. Microbiol., 96: 1361-1366.
Gortel, K (2013). Recognizing pyoderma: more difficult than it may seem. Vet. Clin. North Am.: Small Anim. Pract., 43: 1-18.
Han, JI; Yang, CH and Park, HM (2016). Prevalence and risk factors of Staphylococcus spp. carriage among dogs and their owners: a cross-sectional study. Vet. J., 21: 15-21.
Hartmann, FA; White, DG; West, SE; Walker, RD and Deboer, DJ (2005). Molecular characterization of Staphylococcus intermedius carriage by healthy dogs and comparison of antimicrobial susceptibility patterns to isolates from dogs with pyoderma. Vet. Microbiol., 108: 119-131.
Hauschild, T and Wójcik, A (2007). Species distribution and properties of staphylococci from canine dermatitis. Res. Vet. Sci., 82: 1-6.
Holm, BR; Petersson, U; Mörner, A; Bergström, K; Franklin, A and Greko, C (2002). Antimicrobial resistance in staphylococci from canine pyoderma: a prospective study of first-time and recurrent cases in Sweden. Vet. Record., 15: 600-605.
Huerta, B; Maldonado, A; Ginel, PJ; Tarradas, C; Gómez-Gascón, L; Astorga, RJ and Luque, I (2011). Risk factors associated with the antimicrobial resistance of staphylococci in canine pyoderma. Vet. Microbiol., 150: 302-308.
May, ER (2006). Bacterial skin diseases: current thoughts on pathogenesis and management. Vet. Clin. North Am.: Small Anim. Pract., 36: 185-202.
Miller, WH; Griffin, CE and Campbell, KL (2013). Bacterial skin diseases. In: Miller, W; Griffin, C and Campbell, KM (Eds.), Muller and Kirk’s small animal dermatology. 7th Edn., Philadelphia, W. B. Saunders. PP: 184-198.
Moon, BY; Youn, JH; Shin, S; Hwang, SY and Park, YH (2012). Genetic and phenotypic characterization of methicillin-resistant staphylococci isolated from veterinary hospitals in South Korea. J. Vet. Diagn. Invest., 24: 489-498.
Morris, DO; Rook, KA; Shofer, FS and Rankin, SC (2006). Screening of Staphylococcus aureus, Staphylococcus intermedius, and Staphylococcus schleiferi isolates obtained from small companion animals for antimicrobial resistance: a retrospective review of 749 isolates (2003-04). Vet. Dermatol., 17: 332-337.
Pinchbeck, LR; Cole, LK; Hillier, A; Kowalski, J; Rajala-Schultz, PJ; Bannerman, TL and York, S (2006). Genotypic relatedness of staphylococcal strains isolated from pustules and carriage sites in dogs with superficial bacterial folliculitis. Am. J. Vet. Res., 67: 1337-1346.
Rich, M (2005). Staphylococci in animals: prevalence, identification and antimicrobial susceptibility, with an emphasis on methicillin-resistant Staphylococcus aureus. Br. J. Biomed. Sci., 62: 98-105.
Schmidt, VM; Williams, NJ; Pinchbeck, G; Caroline, E; Shaw, CS; McEwan, N; Dawson, S and Nuttall, T
(2014). Antimicrobial resistance and characterization of staphylococci isolated from healthy Labrador retrievers in the United Kingdom. BMC Vet. Res., 10: 17.
Shimizu, A; Wakita, Y; Nagase, S; Okabe, M; Koji, T; Hayashi, T; Nagase, N; Sasaki, A; Kawano, J; Yamashita, K and Takagi, M (2001). Antimicrobial susceptibility of Staphylococcus intermedius isolated from healthy and diseased dogs. J. Vet. Med. Sci., 63: 357-360.
Simoons-Smit, AM; Savelkoul, PH; Stoof, J; Starink, TM and Vandenbroucke-Grauls, CMJ (2000). Transmission of Staphylococcus aureus between humans and domestic animals in a household. Eur. J. Clin. Microbiol. Infect. Dis., 19: 150-152.
Van Duijkeren, E; Wolfhagen, MJ; Box, AT; Heck, ME; Wannet, WJ and Fluit, AC (2004). Human-to-dog transmission of methicillin-resistant Staphylococcus aureus. Imerg. Infect. Dis., 10: 2235-2237.
Vanni, M; Tognetti, R; Pretti, C; Crema, F; Soldani, G; Meucci, V and Intorre, L (2009). Antimicrobial susceptibility of Staphylococcus intermedius and Staphylococcus schleiferi isolated from dogs. Res. Vet. Sci., 87: 192-195.
Von Eiff, C; Peters, G and Heilmann, C (2002). Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infec. Dis., 2: 677-685.
Weese, JS (2013). The canine and feline skin microbiome in health and disease. Vet. Dermatol., 24: 137-145.
Yoo, JH; Yoon, JW; Lee, SY and Park, HM (2010). High prevalence of fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius isolates from canine pyoderma and otitis externa in veterinary teaching hospital. J. Microbiol. Biotech., 20: 798-802.
Zdovc, I; Ocepek, M; Pirs, T; Krt, B and Pinter, L (2004). Microbiological features of Staphylococcus schleiferi subsp. coagulans, isolated from dogs and possible misidentification with other canine coagulase-positive staphylococci. J. Vet. Med. B. Infect. Dis. Vet. Public Health. 51: 449-454.

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